Results 61 to 70 of about 644,201 (301)

CHO cell lines generated by PiggyBac transposition [PDF]

BMC Proceedings, 2011
A major bottleneck in the manufacture of recombinant therapeutic proteins is the time and effort needed for the generation of stable, high-producing mammalian cell lines. Conventional gene transfer methods for stable cell line generation rely on random transgene integration, resulting in unpredictable and highly variable levels of expression of the ...
Matasci Mattia, Bachmann Virginie, Baldi Lucia, Hacker David L, De Jesus Maria, Wurm Florian M   +5 more
openaire   +3 more sources

Mechanistic Insights into a Synergistic FeOx/Fe‐N4 System for Practical Nitrate Abatement with Value‐Added Ammonia Recovery

Advanced Functional Materials, EarlyView.
This work provides a novel interpretation of the nitrate reduction mechanism on iron oxides (FeOx) by employing constant‐potential density functional calculations and reports the design and synthesis of a robust and high‐performance Fe3O4/Fe‐N4‐C catalyst with remarkable Faradaic efficiency, current density, and stability under practical reaction ...
Qiang Zhou, Hongzhi Liu, Shenglin Liu, Yuwei Liu, Mamiko Nakabayashi, Yunlong Xie, Naoya Shibata, Feng Gong, Jean‐Jacques Delaunay   +8 more
wiley   +1 more source

Nectin-2-mediated entry of a syncytial strain of herpes simplex virus via pH-independent fusion with the plasma membrane of Chinese hamster ovary cells

Virology Journal, 2006
Background Herpes simplex virus (HSV) can utilize multiple pathways to enter host cells. The factors that determine which route is taken are not clear. Chinese hamster ovary (CHO) cells that express glycoprotein D (gD)-binding receptors are model cells ...
Nicola Anthony V, Hollander Aimee M, Patterson Jennifer L, Delboy Mark G   +3 more
doaj   +1 more source

The role of diacylglycerol lipase in constitutive and angiotensin AT(1) receptor-stimulated cannabinoid CB1 receptor activity [PDF]

, 2018
The cannabinoid CB1 receptor (CB1R) is,a G protein-coupled receptor, which couples to the G(i/o) family of heterotrimeric G proteins. The receptor displays both basal and agonist-induced signaling and internalization.
Azpiazu, Bisogno, Bisogno, Bisogno, Bouaboula, Devane, Freund, Hentges, Howlett, Hunyady, Leterrier, Leterrier, MacLennan, Makara, Matsuda, Mechoulam, Neu, Pacher, Pan, Pertwee, Randall, Rinaldi-Carmona, Spaöt, Turu, Vasquez, Vinod, Zhu   +26 more
core   +1 more source

Nanothermometry in Living Cells: Physical Limits, Conceptual and Material Challenges

Advanced Functional Materials, EarlyView.
Heat and temperature are fundamental to life. When nanothermometers began probing regions as small as a living cell, they triggered controversial claims of large intracellular temperature gradients. We review physical constraints energy‐conservation, entropy production, thermodynamic fluctuations, and molecular dynamics.
Taras Plakhotnik
wiley   +1 more source

A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins [PDF]

, 2001
Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions.
Carvalho, L.c.m., Dietrich, Carl Peter, Franco, Celia Regina Cavichlolo, Leite, Edda Lisboa, Nader, Helena Bonciani, Rocha, Hugo Alexandre Oliveira, Trindade, Edvaldo da Silva, Veiga, Silvio Sanches   +7 more
core   +2 more sources

Nucleation‐Controlled Reconstruction of CuOx for Selective CO2 Electroreduction

Advanced Functional Materials, EarlyView.
The ratio of oxygen vacancies (Ov) and exposed Cu2O (111)/(200) of CuOx precatalyst is modulated by nucleation control of Cu(OH)2 precursor. Low Ov ratio and high ratio of Cu2O (111)/(200) in slow‐nucleated CuOx reconstructs to high‐coordinated oxide‐derived copper (OD‐Cu) during electrochemical CO2 reduction reaction (CO2RR) and exhibits enhanced ...
Ying Ying Ch'ng, Zhipeng Ma, Ming Zhang, Shujie Zhou, Varun Kundi, Priyank Vijaya Kumar, Wenyu Zhong, Yuwei Yang, Jitraporn Vongsvivut, Qinfen Gu, Xiaoxuan Luo, Lixue Jiang, Rahman Daiyan, Zhaojun Han, Rose Amal   +14 more
wiley   +1 more source

Bioactivation, Mutagenicity, DNA Damage, and Oxidative Stress Induced by 3,4-Dimethylaniline

Biomolecules
3,4-Dimethylaniline (3,4-DMA) is present in cigarette smoke and widely used as an intermediate in dyes, drugs, and pesticides. Nucleotide excision repair-deficient Chinese hamster ovary (CHO) cells stably transfected with human CYP1A2 and N ...
Mariam R. Habil, Raúl A. Salazar-González, Mark A. Doll, David W. Hein   +3 more
doaj   +1 more source

Functional modulation of the transient outward current Ito by KCNE beta-subunits and regional distribution in human non-failing and failing hearts [PDF]

, 2006
Objectives: The function of Kv4.3 (KCND3) channels, which underlie the transient outward current I,, in human heart, can be modulated by several accessory subunits such as KChIP2 and KCNE1-KCNE5.
Banse, U., Cotella, D., Graf, E., Jost, Norbert, Radicke, S., Ravens, U., Tseng, G., Varró, András, Wettwer, E.   +8 more
core   +1 more source

Electrocatalytic Reduction of CO2 to Ethylene: Catalyst Design and Synchrotron‐Based Characterizations

Advanced Functional Materials, EarlyView.
This review evaluates strategies for electrochemical CO2 reduction to ethylene, focusing on copper‐based catalyst design and microenvironment modulation to achieve industrial‐grade performance. By leveraging operando synchrotron‐based characterizations, we provide a multiscale understanding of dynamic structural transformations and key reaction ...
Meng Zhang, Zuolong Chen, Yimin A. Wu
wiley   +1 more source