Results 71 to 80 of about 188,771 (295)

Enhancing protein production and growth in chinese hamster ovary cells through miR-107 overexpression

AMB Express
Chinese Hamster Ovary (CHO) cells are widely employed as host cells for biopharmaceutical production. The manufacturing of biopharmaceuticals poses several challenges, including restricted growth potential and inadequate productivity of the host cells ...
Maryam Jari, Shahriyar Abdoli, Zahra Bazi, Fatemeh Tash Shamsabadi, Farnaz Roshanmehr, Majid Shahbazi   +5 more
doaj   +1 more source

Adalimumab‐Poloxamer Conjugate for Bio‐Better: Enhanced Stability and Function

Advanced Functional Materials, EarlyView.
Antibody‐polymer conjugates, particularly poloxamer conjugates, enhance antibody stability by improving tolerance to physicochemical stress and attenuating proteolysis by proteases. Furthermore, the higher affinity observed with poloxamer conjugation compared to standard PEGylation results in improved therapeutic efficacy in rheumatoid arthritis mouse ...
Jaewon Roh, Sanghee Lee, Soomin Cho, Kun Na   +3 more
wiley   +1 more source

The Dual-Pseudotyped Lentiviral Vector with VSV-G and Sendai Virus HN Enhances Infection Efficiency through the Synergistic Effect of the Envelope Proteins

Viruses
A gene delivery system utilizing lentiviral vectors (LVs) requires high transduction efficiency for successful application in human gene therapy. Pseudotyping allows viral tropism to be expanded, widening the usage of LVs.
Bat-Erdene Jargalsaikhan, Masanaga Muto, Youngeun Been, Shoma Matsumoto, Eiichi Okamura, Tadanobu Takahashi, Yutaka Narimichi, Yuuki Kurebayashi, Hideyuki Takeuchi, Takashi Shinohara, Ryo Yamamoto, Masatsugu Ema   +11 more
doaj   +1 more source

Embedded 3D‐Coaxial Bioprinting of Stenotic Brain Vessels with a Mechanically Enhanced Extracellular Matrix Bioink for Investigating Hemodynamic Force‐Induced Endothelial Responses

Advanced Functional Materials, EarlyView.
In this study, a physically enhanced vascular dECM bioink and used 3D‐coaxial bioprinting are developed to fabricate mature brain blood vessels for cerebral atherosclerosis research. This model demonstrates that vascular geometry‐induced hemodynamic changes trigger vascular inflammation, ensuring its potential for cerebrovascular research.
Wonbin Park, Min‐Ju Choi, Jae‐Seong Lee, Minjun Ahn, Wonil Han, Ge Gao, Dong‐Woo Cho, Byoung Soo Kim   +7 more
wiley   +1 more source

Synthetic Strategy for mRNA Encapsulation and Gene Delivery with Nanoscale Metal‐Organic Frameworks

Advanced Functional Materials, EarlyView.
This research utilizes the ZIF‐8 for the encapsulation and intracellular delivery of nucleic acids, specifically mRNA, for applications in gene delivery. Integrating PEI addresses the issue of mRNA leakage from ZIF‐8, resulting in the delivery and expression of green fluorescent protein (GFP) in vitro and firefly luciferase in vivo.
Harrison Douglas Lawson, Huy Hoang Nguyen, Keng‐Jung Lee, Nattarat Wongsuwan, Ayesha Tupe, Mengrou Lu, Mariah Lynn Arral, Anne Behre, Zihan Ling, Kathryn Ann Whitehead, Adam Walter Feinberg, Xi Ren, Si‐Yang Zheng   +12 more
wiley   +1 more source

Comprehensive Si Anode Design for Sulfide‐Based all‐Solid‐State Batteries: Insights into Si‐Electrolyte Synergy for Mitigating Contact Loss

Advanced Functional Materials, EarlyView.
Silicon anodes in sulfide‐based all‐solid‐state batteries suffer from interfacial instability caused by volumetric fluctuations and contact degradation, restricting their practical implementation despite high capacity. This study presents design strategies that leverage controlled active material expansion and an elastic recoverable anolyte to form an ...
Youngjin Song, Sungjin Cho, Suhwan Kim, Youyeong Shin, Ikcheon Na, Jongwoo Lim, Yong Min Lee, Soojin Park   +7 more
wiley   +1 more source

An Attempt to Add Biological Functions by Genetic Engineering in Order to Produce High-Performance Bioreactor Cells for Hybrid Artificial Liver: Transfection of Glutamine Synthetase into Chinese Hamster Ovary (CHO) Cell

Cell Transplantation, 1997
In the course of immortalization, hepatocyte cell lines lose their original differentiated functions, such as ammonia removal and urea formation, drug metabolism, serum protein synthesis, etc. (Enosawa et al., Cell Transplant. 5:S39-S40; 1996).
Shin Enosawa, Seiichi Suzuki, Masayuki Fujino, Hiroshi Amemiya, Takeshi Omasa, Shigeo Urayama, Naoko Tanimura, Kenichi Suga   +7 more
doaj   +1 more source

A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins

Brazilian Journal of Medical and Biological Research, 2001
Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions.
H.A.O. Rocha, C.R.C. Franco, E.S. Trindade, L.C.M. Carvalho, S.S. Veiga, E.L. Leite, C.P. Dietrich, H.B. Nader   +7 more
doaj   +1 more source

Harnessing Interfacial Cl− Ions for Concurrent Formate Production at Industrial Level via CO2 Reduction and Methanol Oxidation

Advanced Functional Materials, EarlyView.
The replacement of the anodic OER in CO2RR with energy‐saving methanol oxidation reaction and the creation of a favorable microenvironment through interfacial Cl− ions enable concurrent formate production at industrial‐level current densities. Abstract The efficient electrocatalytic conversion of CO2 to formate is often impeded by the high energy ...
Yiqun Chen, Yan Zhang, Zhen Li, Biao Feng, Mengting Li, Qiang Wu, Zheng Hu, Lawrence Yoon Suk Lee   +7 more
wiley   +1 more source

Sonication‐Assisted Palladium Nanogaps in a Capacitive Structure: A Tunable and Reliable Solution for Sensitive Hydrogen Monitoring

Advanced Functional Materials, EarlyView.
This study presents a capacitive hydrogen sensor with a simple structure and process. Sonication‐induced Pd nanogaps modulate the effective electrode area in response to hydrogen concentration, resulting in capacitance changes within a range predefined by physical parameters.
Sang‐kil Lee, Joonho Lee, Jiwoo Hong, Seyoung Park, Eugene Cho, Seunghyun Lee, Keunhyung Lee, Hyun‐Sook Lee, Kyu Hyoung Lee, Wooyoung Lee   +9 more
wiley   +1 more source