Analysis of ligand recognition by choline O-acetyltransferase reveals thiol-reactive assay interference and weak ligand affinity in solution. [PDF]
J Biol ChemCholine O-acetyltransferase (ChAT) catalyzes the biosynthesis of acetylcholine and is a cysteine-rich enzyme that has been investigated using a range of biochemical, biophysical and structural approaches. Existing ChAT ligands rely on electrophilic or unstable scaffolds that limit their suitability for biological systems.
Forsgren N +9 more
europepmc +3 more sources
Molecules, 2022 This study was conducted to evaluate the protective effect of Juglans regia (walnut, Gimcheon 1ho cultivar, GC) on high-fat diet (HFD)-induced cognitive dysfunction in C57BL/6 mice.
Jong Hyun Moon +12 more
doaj +1 more source
Ocimum sanctum Linn. stimulate the expression of choline acetyltransferase on the human cerebral microvascular endothelial cells [PDF]
Veterinary World, 2016 Aim: This research was conducted to identify the expression of choline acetyltransferase (ChAT) in human cerebral microvascular endothelial cells (HCMECs) and to clarify the capability of Ocimum sanctum Linn.
Dwi Liliek Kusindarta +2 more
doaj +1 more source
Differentiation of two human neuroblastoma cell lines alters SV2 expression patterns
Cellular & Molecular Biology Letters, 2021 Background The synaptic vesicle glycoprotein 2 (SV2) family is essential to the synaptic machinery involved in neurotransmission and vesicle recycling. The isoforms SV2A, SV2B and SV2C are implicated in neurological diseases such as epilepsy, Alzheimer’s
Emilia Lekholm +4 more
doaj +1 more source
Exploring the neurogenic differentiation of human dental pulp stem cells.
PLoS ONE, 2022 Human dental pulp stem cells (hDPSCs) have increasingly gained interest as a potential therapy for nerve regeneration in medicine and dentistry, however their neurogenic potential remains a matter of debate.
Arwa A Al-Maswary +5 more
doaj +1 more source
Integrating precision medicine in the study and clinical treatment of a severely mentally ill person [PDF]
PeerJ, 2013 Background. In recent years, there has been an explosion in the number of technical and medical diagnostic platforms being developed. This has greatly improved our ability to more accurately, and more comprehensively, explore and characterize human ...
Jason A. O’Rawe +8 more
doaj +2 more sources
Molecular weight determinations of soluble and membrane-bound fractions of choline O-acetyltransferase in rat brain [PDF]
The Journal of Neuroscience, 1985 Three fractions of choline O-acetyltransferase (ChAT) (EC 2.3.1.6) were solubilized from a nerve ending fraction of rat forebrain using three sequential washes of an increasingly chaotrophic nature (100 mM sodium phosphate, pH 7.4; 500 mM NaCl; 2% Triton DN-65) as previously described (Benishin, C.G., and P.T. Carroll (1983) J. Neurochem. 41: 1030–1039)
M, Badamchian, P T, Carroll
openaire +2 more sources
Characterisation of Sulfolobus solfataricus Ard1, a promiscuous N-acetyltransferase [PDF]
, 2008 Compaction of DNA into chromatin is an important feature of every living cell. This compaction phenomenon is brought about and maintained by a variety of DNA binding proteins, which have evolved to suit the specific needs of the different cell types ...
Mackay, Dale Tara
core +2 more sources
Journal of Pharmacological Sciences, 2011 .: We investigated the effect of kangen-karyu (KK), a Chinese herbal prescription, on cognitive deficits and central cholinergic systems of type 2 diabetic db/db mice. Seven-week-old db/db (Y-db/db) mice received daily administration of test drugs during
Qi Zhao +7 more
doaj +1 more source
Monoclonal antibodies to choline acetyltransferase of rat brain [PDF]
, 1983 Monoclonal antibodies to rat brain choline acetyltransferase were produced by the hybridoma technique. Two stable cell lines, Ab-57 and Ab-60, secreted immunoglobulin of subclass IgG1.
Tomoyuki Ichikawa +5 more
core +1 more source