Results 241 to 250 of about 30,507 (276)
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Chondroitin sulfate proteoglycans inhibit oligodendrocyte myelination through PTPσ
Experimental Neurology, 2013CNS damage often results in demyelination of spared axons due to oligodendroglial cell death and dysfunction near the injury site. Although new oligodendroglia are generated following CNS injury and disease, the process of remyelination is typically incomplete resulting in long-term functional deficits.
James C, Pendleton +6 more
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Deglycosylation of chondroitin sulfate proteoglycan and derived peptides
Biochemistry, 1990In order to define the domain structure of proteoglycans as well as identify primary amino acid sequences specific for attachment of the various carbohydrate substituents, reliable techniques for deglycosylating proteoglycans are required. In this study, deglycosylation of cartilage chondroitin sulfate proteoglycan (CSPG) with minimal core protein ...
S C, Campbell +2 more
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Chondroitin sulfate proteoglycan specific to retinal horizontal neurons
Journal of Comparative Neurology, 1998ABSTRACT Proteoglycans (PGs) are a diverse group of highly glycosylated macromolecules that are implicated in the development and maintenance of neuronal circuitry. With its highly ordered, layered structure, the retina ideally serves to define the synthesis, processing, and distribution of these molecules within a specific cellular subpopulation.
C, Williams +3 more
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Isolation, Purification, and Analysis of Chondroitin Sulfate Proteoglycans
2006Publisher Summary This chapter discusses widely applicable procedures for the purification and characterization of chondroitin sulfate proteoglycans (CSPGs), using several typical CSPG species as examples. The purification of CSPGs is an essential step to determine the structure and function of CSPGs.
Fumiko, Matsui, Atsuhiko, Oohira
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Isolation of a chondroitin sulfate-dermatan sulfate proteoglycan from bovine aorta
Archives of Biochemistry and Biophysics, 1975Abstract Material containing proteoglycans was extracted from bovine aorta by the dissociative solvent 3.0 m MgCl2. The proteoglycan that remained in solution at low ionic strength was purified by isopycnic CsCl centrifugation (ϱ, 1.75 – 1.89 g/ml).
K C, Ehrlich +2 more
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Primary mesenchyme cell migration requires a chondroitin sulfate/dermatan sulfate proteoglycan
Developmental Biology, 1991Primary mesenchyme cell migration in the sea urchin embryo is inhibited by sulfate deprivation and exposure to exogenous beta-D-xylosides, two treatments known to disrupt proteoglycan synthesis. We show that in the developing sea urchin, exogenous xyloside affects the synthesis by the primary mesenchyme cells of a very large, cell surface chondroitin ...
M C, Lane, M, Solursh
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Detection of Chondroitin Sulfate Proteoglycan 4 (CSPG4) in Melanoma
2013The tumor antigen chondroitin sulfate proteoglycan 4 (CSPG4) appears to be a useful biomarker to identify melanoma cells and an attractive target to apply antibody-based immunotherapy for the treatment of melanoma. Here we described the reverse transcription-polymerase chain reaction (RT-PCR) method and the immunohistochemical (IHC) staining method to ...
Wang, Yangyang +3 more
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Chondroitin Sulfate Proteoglycan Elevates Cytoplasmic Calcium in DRG Neurons
Developmental Biology, 1994Proteoglycans have been implicated in neuronal path-finding during development, yet related second messenger and signaling systems are unknown. We have used the calcium indicator fura-2/AM to monitor cytoplasmic calcium ion concentration ([Ca2+]i) in chick dorsal root ganglion (DRG) neuronal growth cones elongating on laminin during contact with ...
D M, Snow +4 more
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Chondroitin sulfate proteoglycan of human leukocytes
Biochimica et Biophysica Acta (BBA) - General Subjects, 1969Abstract 1. 1. A chondroitin sulfate proteogylcan was isolated from leukocyte granules and partly characterized. It had a low protein content and a relatively low molecular size. 2. 2. The incorporation of [ 35 S]sulfate, [ 14 C]glucosamine and [ 3 H]serine into chondroitin sulfate was inhibited by puromycin, indicating that the elongation of
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Major chondroitin sulfate proteoglycans identified in L6J1 myoblast culture
Biochemistry (Moscow), 2011The major proteoglycans from L6J1 rat myoblast culture were identified. The proteoglycans were isolated from different constituents of cell culture: culture medium, extracellular matrix (ECM), and myoblasts. To identify their core proteins, the proteoglycans were treated with enzymes specifically digesting chondroitin/dermatan sulfates or chondroitin ...
I I, Ermakova +5 more
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