Results 11 to 20 of about 4,684 (170)

Design, selection, and characterization of a split chorismate mutase. [PDF]

Protein Sci, 2010
AbstractSplit proteins are versatile tools for detecting protein–protein interactions and studying protein folding. Here, we report a new, particularly small split enzyme, engineered from a thermostable chorismate mutase (CM). Upon dissecting the helical‐bundle CM from Methanococcus jannaschii into a short N‐terminal helix and a 3‐helix segment and ...
Müller MM, Kries H, Csuhai E, Kast P, Hilvert D.   +4 more
europepmc   +6 more sources

A broadly conserved fungal chorismate mutase targets the plant shikimate pathway to regulate salicylic acid production and other secondary metabolites [PDF]

mBio
The molecular dynamics of plant-pathogen interactions are complex, involving a constant defense race between host plants and pathogens. Plants deploy antimicrobial metabolites and physiological responses to inhibit pathogens, while pathogens counteract ...
Dandan Shao, Nathaniel M. Westrick, Wende Liu, Oded Yarden, Jun Zhao, Yuri Kimura, Hiroshi Maeda, Damon L. Smith, Mehdi Kabbage, Martin B. Dickman   +9 more
doaj   +2 more sources

A petunia chorismate mutase specialized for the production of floral volatiles. [PDF]

Plant J, 2010
In Petunia x hybrida cv. 'Mitchell Diploid' floral fragrance is comprised of 13 volatile benzenoids/phenylpropanoids derived from the aromatic amino acid phenylalanine. Several genes involved in the direct synthesis of individual floral volatile benzenoid/phenylpropanoid (FVBP) compounds, i.e.
Colquhoun TA, Schimmel BC, Kim JY, Reinhardt D, Cline K, Clark DG.   +5 more
europepmc   +5 more sources

Methanol-based biosynthesis of p-coumaric acid by engineered Pichia pastoris [PDF]

Bioresources and Bioprocessing
p-Coumaric acid (p-CA) is a key aromatic precursor for the biosynthesis of flavonoids, stilbenoids, and other high-value phenylpropanoids. While microbial production of p-CA typically relies on sugar-based substrates, methanol offers a sustainable and ...
Mengyuan Chen, Jiayu Fang, Shuxian Wang, Guoxia Liu, Yanping Zhang, Yin Li, Kaizhi Jia, Taicheng Zhu   +7 more
doaj   +2 more sources

A fungal effector promotes infection via stabilizing a negative regulatory factor of chloroplast immunity [PDF]

Nature Communications
Chloroplasts are crucial players in immunity and photosynthesis. However, how chloroplasts arrange the transition between photosynthesis and immunity and how pathogens manipulate this transition remains elusive.
Kunqin Xiao, Feng Yang, Wenjing Cui, Anmo Li, Jeffrey A. Rollins, Jinxin Guo, Xinhua Sun, Fengting Wang, Xiaojie Wang, Xun Xu, Yanhua Zhang, Xianghui Zhang, Jinliang Liu, Hongyu Pan   +13 more
doaj   +2 more sources

Accumulation of Polyphenolics and Differential Expression of Genes Related to Shikimate Pathway during Fruit Development and Maturation of Chinese Olive (Canarium album)

Agronomy, 2023
Phenolics in the Chinese olive (Canarium album (Lour.) Raeusch) fruit significantly affect its flavor and quality. The shikimate pathway is a bridge connecting primary metabolism and secondary metabolism through which fixed carbon can be transformed into
Jingrong Cai, Naiyu Wang, Junyue Zhao, Yan Zhao, Rong Xu, Fanghao Fu, Tengfei Pan, Yuan Yu, Zhixiong Guo, Wenqin She   +9 more
doaj   +1 more source

Mapping enzymatic catalysis using the effective fragment molecular orbital method: towards all ab initio biochemistry. [PDF]

PLoS ONE, 2013
We extend the Effective Fragment Molecular Orbital (EFMO) method to the frozen domain approach where only the geometry of an active part is optimized, while the many-body polarization effects are considered for the whole system.
Casper Steinmann, Dmitri G Fedorov, Jan H Jensen   +2 more
doaj   +1 more source

Functional mapping of protein-protein interactions in an enzyme complex by directed evolution. [PDF]

PLoS ONE, 2014
The shikimate pathway enzyme chorismate mutase converts chorismate into prephenate, a precursor of Tyr and Phe. The intracellular chorismate mutase (MtCM) of Mycobacterium tuberculosis is poorly active on its own, but becomes >100-fold more efficient ...
Kathrin Roderer, Martin Neuenschwander, Giosiana Codoni, Severin Sasso, Marianne Gamper, Peter Kast   +5 more
doaj   +1 more source

The Sporisorium reilianum Effector Vag2 Promotes Head Smut Disease via Suppression of Plant Defense Responses

Journal of Fungi, 2022
Genome comparison between the maize pathogens Ustilago maydis and Sporisorium reilianum revealed a large diversity region (19-1) containing nearly 30 effector gene candidates, whose deletion severely hampers virulence of both fungi.
Yulei Zhao, Nisha Agrawal, Hassan Ghareeb, Mohammad Tanbir Habib, Sascha Dickmeis, Jens Schwachtje, Tim E. Iven, Joachim Kopka, Ivo Feussner, Jan Schirawski   +9 more
doaj   +1 more source

Meloidogyne javanica Chorismate Mutase 1 Alters Plant Cell Development

Molecular Plant-Microbe Interactions, 2003
Root-knot nematodes are obligate plant parasites that alter plant cell growth and development by inducing the formation of giant cells for feeding. Nematodes inject secretions from their esophageal glands through their stylet and into plant cells to ...
Elizabeth A. Doyle, Kris N. Lambert
doaj   +1 more source