Results 231 to 240 of about 781,130 (308)

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

Advanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son, Goeun Kim, Jungjoon K. Lee   +2 more
wiley   +1 more source

Epigenetic remodeling during early embryonic development. [PDF]

Front Cell Dev Biol
Wan X, Zhang S, Li J, Kong D, Chen M.
europepmc   +1 more source

Single‐Cell Transcriptomics Reveals FLS2‐Dependent Hypoxia Signaling and ERF13‐Mediated Transcription During flg22‐Triggered Immunity

Advanced Science, EarlyView.
This study employs sc‐RNA sequencing, genetics, and phenotyping to systematically map the cell‐type‐specific immune responses triggered by flg22. It reveals FLS2‐dependent transcriptional reprogramming in epidermal and mesophyll cells, and uncovers crosstalk between immune and hypoxia signaling pathways.
Yaping Zhou, Aizhi Qin, Mengfan Li, Qianli Zhao, Luyao Kong, Lulu Yan, Chunyang Li, Hao Liu, Yinpeng Zhang, Jiani Long, Mengyu Liao, Mengmeng Zhou, Xiaoli Fan, Baozhen Wang, Wenxuan Kang, Shui Wang, Zhixin Liu, Xuwu Sun   +17 more
wiley   +1 more source

Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids. [PDF]

PLoS One
Zambrano Siri RT, Beati P, Inchausti L, Smircich P, Alonso GD, Ocampo J.   +5 more
europepmc   +1 more source

The FOXC2‐LAMA4 Axis Orchestrates Vasculogenic Mimicry and Immunosuppressive Niche Formation to Drive Metastatic Cascade in Renal Cell Carcinoma

Advanced Science, EarlyView.
Advanced clear cell renal cell carcinoma metastasis is driven by a FOXC2+ tumor subpopulation. These cells execute vasculogenic mimicry for dissemination and secrete LAMA4. LAMA4 engages macrophage ITGA6, activating the STAT6/GATA3 axis to polarize macrophages into a pro‐metastatic, immunosuppressive phenotype.
Jiaxi Yao, Tong Xu, Chengyuan Wang, Chenyuan Wang, Junfeng Xie, Rui Zheng, Ke Wang, Xingzuo Jiang, Zewei Hu, Hongwei Jing, Lin Li, Tao Liu   +11 more
wiley   +1 more source

PETScan: score-based genome-wide association analysis of RNA-Seq and ATAC-Seq data. [PDF]

Bioinformatics
Hao Y, Kafri T, Zou F.
europepmc   +1 more source

GNL3 Orchestrates AR Transcriptional Programs to Drive Castration‐Resistant Prostate Cancer and Immune Evasion

Advanced Science, EarlyView.
GNL3 is a novel AR coregulator with dual coactivator and corepressor functions in prostate cancer (PCa). Our study uncovers a previously unrecognized mechanism by which the AR transcriptional complex integrates oncogenic signaling and immune suppression.
Cuiting Zhang, Tin Long Cheong, Nitin Narwade, Dandan Dong, Mokan Deng, Zhengqiang Miao, Zhaoqiang Ding, Wenchao Li, Kate Man Kei Lei, Gong‐Hong Wei, Terence Chuen Wai Poon, Chu‐Xia Deng, Edwin Cheung   +12 more
wiley   +1 more source

Integrated chromatin and transcriptomic profiling reveals sex-specific mechanisms of gene regulation in hepatic nutrient responses. [PDF]

PLoS Biol
Zhang Z, Su V, Wiese CB, Cheng L, Wang D, Cui Y, Kallapur A, Kim J, Wu X, Tran PH, Zhou Z, Casero D, Li W, Hevener AL, Reue K, Sallam T.   +15 more
europepmc   +1 more source

Oral Streptococcus salivarius Couples Neutrophil IRGM1 Signaling to NET Formation and Colorectal Cancer Metastasis

Advanced Science, EarlyView.
The oral bacterium Streptococcus salivarius promotes colorectal cancer metastasis by inducing neutrophil extracellular trap (NET) formation. Mechanistically, IRGM1–IQGAP1 interaction activates Wnt5a–PI3K/AKT signaling in neutrophils, driving NET‐mediated tumor progression.
Fengyi Liu, Yuan Wang, Hengxuan Cai, Qianwen Yue, Xueli Liu, Xuanwen Bao, Zeng Wang, Xuehan Li, Lai Wei, Zhihua Zhang, Fan Xing, Yi Xu, Xianjun Li, Wei Xu   +13 more
wiley   +1 more source

A pipeline for single-cell chromatin accessibility data analysis. [PDF]

Blood Sci
Zhang M, Chen C.
europepmc   +1 more source