Results 31 to 40 of about 636,375 (263)

Quantitative analysis of chromatin compaction in living cells using FLIM-FRET [PDF]

, 2009
We present a quantitative Forster resonance energy transfer (FRET)-based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. The assay uses a human cell
Angus I. Lamond, Norman, David G., James, John, Lleres, David, Lamond, Angus, I, John James, David Llères, Swift, Sam, David G. Norman, Norman, David, G, Lamond, Angus I.; id_orcid, Sam Swift   +11 more
core   +1 more source

Identification and validation of transcription factors that regulate chromatin dynamics [PDF]

, 2012
Gene expression has to be tightly regulated during all cellular processes. During embryonic development differentiating cells loose their developmental potential and acquire specific functions by activating lineage-specific genes.
Schöler, Anne
core   +1 more source

Nuclear factor I genomic binding associates with chromatin boundaries [PDF]

, 2013
BACKGROUND: The Nuclear Factor I (NFI) family of DNA binding proteins (also called CCAAT box transcription factors or CTF) is involved in both DNA replication and gene expression regulation.
Armelle Gaussin, Schmid, Christoph D., Mermod, Nicolas, Jozef Adamcik, Ambrosini, Giovanna, Schmid, C. D., Pjanic, P., Giovanna Ambrosini, Plasari, Genta, Genta Plasari, Bucher, P., Adamcik, Jozef, Kerschgens, J., Christoph D Schmid, Petar Pjanic, Pjanic, Petar, Kerschgens, Jan, Ambrosini, G., Pjanic, Milos, Gaussin, Armelle, Gaussin, A., Pjanic, M., Philipp Bucher, Plasari, G., Jan Kerschgens, Adamcik, J., Giovani Dietler, Bucher, Philipp, Milos Pjanic, Dietler, Giovani, Dietler, G., Schmid, C.D., Nicolas Mermod, Mermod, N., Ambrosini G, Adamcik J, Plasari G, Dietler G, Pjanic P, Gaussin A, Bucher P, Schmid CD, Kerschgens J, Pjanic M, Mermod N   +44 more
core   +2 more sources

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Kinetic control of eukaryotic chromatin structure by recursive topological restraints [PDF]

, 2008
Chromatin structure undergoes many changes during the cell cycle and in response to regulatory events. A basic unit of chromatin organization is the nucleosome core particle.
Dominika Borek, Zbyszek Otwinowski
core  

Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity [PDF]

, 2011
Genomic maps of chromatin modifications have provided evidence for the partitioning of genomes into domains of distinct chromatin states, which assist coordinated gene regulation.
West, A., Ma, M.K-W., Adam G West, Heath, C., Carol Heath, Adam G. West, Meiji Kit-Wan Ma, Hair, A., Alan Hair, West Adam G., Heath Carol, Ma Meiji Kit-Wan, Hair Alan   +12 more
core   +2 more sources

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Histone modifications form a cell-type-specific chromosomal bar code that persists through the cell cycle

Scientific Reports, 2021
Chromatin configuration influences gene expression in eukaryotes at multiple levels, from individual nucleosomes to chromatin domains several Mb long.
John A. Halsall, Simon Andrews, Felix Krueger, Charlotte E. Rutledge, Gabriella Ficz, Wolf Reik, Bryan M. Turner   +6 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

The Genome in a Three-Dimensional Context: Deciphering the Contribution of Noncoding Mutations at Enhancers to Blood Cancer

Frontiers in Immunology, 2020
Associations between blood cancer and genetic predisposition, including both inherited variants and acquired mutations and epimutations, have been well characterized.
Llorenç Rovirosa, Alberto Ramos-Morales, Biola M. Javierre, Biola M. Javierre   +3 more
doaj   +1 more source