Results 21 to 30 of about 621,772 (309)

Structural basis of RNA polymerase II transcription on the chromatosome containing linker histone H1

Nature Communications, 2022
The mechanism by which RNAPII transcribes the DNA in the chromatosome with H1 has remained enigmatic. Here the authors present the cryo-EM structures of the RNAPII-chromatosome complexes, and explain how RNAPII is regulated by H1 in chromatin.
Rina Hirano, Haruhiko Ehara, Tomoya Kujirai, Tamami Uejima, Yoshimasa Takizawa, Shun-ichi Sekine, Hitoshi Kurumizaka   +6 more
doaj   +1 more source

Targeting the Nucleosome Acidic Patch by Viral Proteins: Two Birds with One Stone?

mBio, 2022
The past decade illuminated the H2A-H2B acidic patch as a cornerstone for both nucleosome recognition and chromatin structure regulation. Higher-order folding of chromatin arrays is mediated by interactions of histone H4 tail with an adjacent nucleosome ...
Floriane Lagadec, Vincent Parissi, Paul Lesbats   +2 more
doaj   +1 more source

The Adenomatous Polyposis Coli protein contributes to normal compaction of mitotic chromatin [PDF]

, 2012
The tumour suppressor <i>Adenomatous Polyposis Coli</i> (APC) is required for proper mitosis; however, the exact role of APC in mitosis is not understood.
Newton, Ian P., Nathke, Inke S.; id_orcid, Ashwat Visvanathan, Daniel Klotz (158368), Inke S Näthke, Visvanathan, Ashwat, Gaganmeet S. Chadha (158365), Klotz Daniel, Swedlow, Jason R.; id_orcid, Visvanathan, A., Näthke, I.S., Chadha, Gaganmeet S., Gaganmeet S Chadha, Lamond Angus, Angus Lamond (158376), Dikovskaya Dina, Visvanathan Ashwat, Dikovskaya, Dina, Näthke Inke S., Lamond, A., Swedlow Jason R., Dina Dikovskaya (158354), Jason R. Swedlow (158382), Inke S. Näthke (158386), Newton Ian P., Lamond, Angus; id_orcid, Newton, I.P., Khoudoli, Guennadi, Khoudoli, G., Angus Lamond, Guennadi Khoudoli (158358), Dikovskaya, D., Daniel Klotz, Klotz, D., Jason R Swedlow, Khoudoli Guennadi, Chadha, G.S., Guennadi Khoudoli, Klotz, Daniel, Dina Dikovskaya, Ian P. Newton (158362), Chadha Gaganmeet S., Ashwat Visvanathan (158372), Swedlow, J.R., Ian P Newton   +44 more
core   +1 more source

Reverse Phase-high-performance Liquid Chromatography (RP-HPLC) Analysis of Globin Chains from Human Erythroid Cells

Bio-Protocol, 2021
β-hemoglobinopathies are severe genetic disorders characterized either by the abnormal synthesis of the adult β-globin chains of the hemoglobin (Hb) tetramer (βS-globin chains) in sickle cell disease (SCD) or by the reduced β-globin production in β ...
Anne Chalumeau, Giacomo Frati, Elisa Magrin, Annarita Miccio   +3 more
doaj   +1 more source

Quantitative analysis of chromatin compaction in living cells using FLIM-FRET [PDF]

, 2009
We present a quantitative Forster resonance energy transfer (FRET)-based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. The assay uses a human cell
Angus I. Lamond, Norman, David G., James, John, Lleres, David, Lamond, Angus, I, John James, David Llères, Swift, Sam, David G. Norman, Norman, David, G, Lamond, Angus I.; id_orcid, Sam Swift   +11 more
core   +1 more source

Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements

Epigenetics & Chromatin, 2018
Background Linker histones establish and maintain higher-order chromatin structure. Eleven linker histone subtypes have been reported in mammals. HILS1 is a spermatid-specific linker histone, and its expression overlaps with the histone–protamine ...
Laxmi Narayan Mishra, Vasantha Shalini, Nikhil Gupta, Krittika Ghosh, Neeraj Suthar, Utsa Bhaduri, M. R. Satyanarayana Rao   +6 more
doaj   +1 more source

Transposable Element Dynamics and Regulation during Zygotic Genome Activation in Mammalian Embryos and Embryonic Stem Cell Model Systems

Stem Cells International, 2021
Transposable elements (TEs) are mobile genetic sequences capable of duplicating and reintegrating at new regions within the genome. A growing body of evidence has demonstrated that these elements play important roles in host genome evolution, despite ...
Yixuan Low, Dennis Eng Kiat Tan, Zhenhua Hu, Shawn Ying Xuan Tan, Wee-Wei Tee   +4 more
doaj   +1 more source

Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues

Microbial Cell, 2023
Enhanced levels of acetic acid reduce the activity of yeast strains employed for industrial fermentation-based applications. Therefore, unraveling the genetic factors underlying the regulation of the tolerance and sensitivity of yeast towards acetic acid
Nitu Saha, Swati Swagatika, Raghuvir Singh Tomar   +2 more
doaj   +1 more source

Function of the upstream activating factor in chromatin structure organization and transcriptional regulation at the yeast ribosomal DNA [PDF]

, 2010
Chromatin is the template of all processes involved in DNA metabolism in the eukaryotic cell. Accordingly, chromatin is a dynamic structure which changes in its composition and posttranslational modification correlating with the functional state of a ...
Götze, Hannah
core   +1 more source

NoRC, a novel chromatin remodeling complex involved in ribosomal RNA gene silencing [PDF]

, 2004
Regulation of gene expression takes place in the nucleus in a highly structured and condensed nucleoprotein environment, called chromatin (Felsenfeld and Groudine, 2003; Khorasanizadeh, 2004; Vaquero et al., 2003).
Strohner, Ralf
core   +1 more source