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Chromatin Immunoprecipitation Using Microarrays
2009Chromatin immunoprecipitation (ChIP) is a powerful procedure to investigate the interactions between proteins and DNA. ChIP-chip combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in vivo.
Mickaël, Durand-Dubief, Karl, Ekwall
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Chromatin RNA Immunoprecipitation (ChRIP)
2017Researchers have recently had a growing interest in understanding the functional role of long noncoding RNAs (lncRNAs) in chromatin organization. Accumulated evidence suggests lncRNAs could act as interphase molecules between chromatin and chromatin remodelers to define the epigenetic code.
Tanmoy, Mondal +2 more
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Topoisomerase II Chromatin Immunoprecipitation
2017Chromatin immunoprecipitation is a method to isolate a protein of interest coupled to DNA following cross-linking with formaldehyde and to quantify the relative abundance or occupancy of the protein at specific genomic loci. After immunoprecipitation of protein-DNA complexes protein-DNA cross-links are reversed and the DNA is extracted. Various methods
Smith KA, Cowell IG, Austin CA
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Putting chromatin immunoprecipitation into context
Journal of Cellular Biochemistry, 2009AbstractChromatin immunoprecipitation (ChIP), when paired with sequencing or arrays, has become a method of choice for the unbiased identification of genomic‐binding sites for transcription factors and epigenetic marks in various model systems. The data generated is often then interpreted by groups seeking to link these binding sites to the expression ...
Zecchini, Vincent, Mills, Ian G
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Chromatin Immunoprecipitation in Macrophages
2018Macrophages are highly polymorphic depending upon their cellular origin and their tissue environment. The different forms that a macrophage can adopt fundamentally reflect different transcription patterns. In addition, macrophages are exquisitely sensitive to a wide variety of signals coming from either infectious agents or damaged tissues. Most of the
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Chromatin Immunoprecipitation (ChIP)
2020Chromatin immunoprecipitation or ChIP is an excellent method of investigation of the specific protein interaction and its altered forms with DNA region. These interactions have a significant role in various cellular processes such as replication, transcription, DNA damage repair, genome stability, gene regulation and segregation at mitosis.
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Chromatin Immunoprecipitation Assays
2007Gene expression pattern in cancer cells differ significantly from their normal counter parts, owing to mutations in oncogenes and tumor suppressor genes, their downstream targets, or owing to increased proliferation, and altered apoptotic potential. Various microarray based techniques have been widely utilized to study the differential expression of ...
Piyali Dasgupta, Srikumar P. Chellappan
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Chromatin Immunoprecipitation of Mouse Embryos
2011During prenatal development, a large number of different cell types are formed, the vast majority of which contain identical genetic material. The basis of the great variety in cell phenotype and function is the differential expression of the approximately 25,000 genes in the mammalian genome.
Anne K, Voss +4 more
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Chromatin Immunoprecipitation in Mammalian Cells
2009The ensemble of the genes in the mammalian genome is organized into a structure of DNA and proteins known as chromatin. The control of gene expression by the proteins that bind to chromatin regulates many cell processes, such as differentiation and proliferation.
Amy, Svotelis +2 more
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Chromatin Immunoprecipitation in Skeletal Myoblasts
2018Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation.
Vinay Kumar, Rao +2 more
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