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Chromatin Immunoprecipitation in Chloroplasts
Current Protocols, 2022AbstractChromatin is the genetic material assembled by nucleic acids (including DNA and RNA) and proteins. The biological functions of chromatin are highly dependent on the interaction between DNA (and/or RNA) and proteins that bind to it. Chromatin immunoprecipitation (ChIP) is a powerful technique for evaluating these interactions and has been widely
Wenjie, Wang +2 more
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2013
Recent studies have elucidated molecular mechanisms underlying the transcriptional control of metabolism in complex metabolic disorders such as metabolic syndrome and atherosclerosis. Chromatin immunoprecipitation (ChIP) is an important technique to study protein-DNA interactions in vivo.
Grant D, Barish, Rajenda K, Tangirala
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Recent studies have elucidated molecular mechanisms underlying the transcriptional control of metabolism in complex metabolic disorders such as metabolic syndrome and atherosclerosis. Chromatin immunoprecipitation (ChIP) is an important technique to study protein-DNA interactions in vivo.
Grant D, Barish, Rajenda K, Tangirala
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Chromatin Preparation and Chromatin Immunoprecipitation from Drosophila Heads
Methods in Molecular Biology, 2023 Chromatin immunoprecipitation (ChIP) is a widely used method to map protein-DNA interactions in vivo. Formaldehyde cross-linked chromatin is fragmented, and the protein of interest is immunoprecipitated using a specific antibody.
Davide Andrenacci, Filippo M Cernilogar
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2018
Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome.
Julio C, Ayala +2 more
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Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome.
Julio C, Ayala +2 more
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High-resolution chromatin immunoprecipitation [PDF]
Nature Methods, 2012 Tal Nawy
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Immunoprecipitation of chromatin
1996Publisher Summary This chapter discusses the immunoprecipitation of chromatin. The chapter discusses three standard protocols, all of which are used to analyze the role of specific proteins within defined regions of the eukaryotic genome. It analyzes both the DNA and the protein after immunoprecipitation.
L P, O'Neill, B M, Turner
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Chromatin Immunoprecipitation (ChIP)
Cold Spring Harbor Protocols, 2009INTRODUCTIONChromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with
Michael F, Carey +2 more
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Chromatin Immunoprecipitation Assays
2004Chromatin immunoprecipitation (ChIP) is a powerful tool to study protein-DNA interaction and is widely used in many fields to study proteins associated with chromatin, such as histone and its isoforms and transcription factors, across a defined DNA domain.
Yan, Yan, Haobin, Chen, Max, Costa
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Chromatin Immunoprecipitation Using Microarrays
Methods in Molecular Biology, 2009Chromatin immunoprecipitation (ChIP) is a powerful procedure to investigate the interactions between proteins and DNA. ChIP-chip combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in vivo.
Karl Ekwall, Mickaël Durand-Dubief
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Native Chromatin Immunoprecipitation
2004Chromatin immunoprecipitation (ChIP) is a technique widely used for determining the genomic location of modified histones and other chromatin-associated factors. Here we describe the methodology we have used in our laboratory for the immunoprecipitation of chromatin isolated from cells in the absence of crosslinking.
Thorne, Alan, Myers, Fiona, Hebbes, Tim
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