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Chromatin Immunoprecipitation and Circadian Rhythms
2022Organisms exhibit daily changes of physiology and behavior to exert homeostatic adaptations to day-night cycles. The cyclic fluctuation also takes place at transcriptional levels, giving rise to rhythmic gene expression. Central to this oscillatory transcription is the core clock machinery which constitutes a circuit of transcriptional-translational ...
Kenichiro, Kinouchi +2 more
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Nanobody-Based Chromatin Immunoprecipitation
2012Chromatin immunoprecipitation (ChIP), followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq), is becoming a widely used powerful method for the analysis of the in vivo DNA-protein interactions at genomic scale.The success of ChIP largely depends on the quality of antibodies.
NGUYEN DUC, Trong +5 more
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Putting chromatin immunoprecipitation into context
Journal of Cellular Biochemistry, 2009AbstractChromatin immunoprecipitation (ChIP), when paired with sequencing or arrays, has become a method of choice for the unbiased identification of genomic‐binding sites for transcription factors and epigenetic marks in various model systems. The data generated is often then interpreted by groups seeking to link these binding sites to the expression ...
Zecchini, Vincent, Mills, Ian G
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Chromatin Immunoprecipitation in Macrophages
2018Macrophages are highly polymorphic depending upon their cellular origin and their tissue environment. The different forms that a macrophage can adopt fundamentally reflect different transcription patterns. In addition, macrophages are exquisitely sensitive to a wide variety of signals coming from either infectious agents or damaged tissues. Most of the
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Chromatin Immunoprecipitation in Mammalian Cells
2009The ensemble of the genes in the mammalian genome is organized into a structure of DNA and proteins known as chromatin. The control of gene expression by the proteins that bind to chromatin regulates many cell processes, such as differentiation and proliferation.
Amy, Svotelis +2 more
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Histone Native Chromatin Immunoprecipitation
2018Chromatin immunoprecipitation (ChIP) is becoming the standard method to study genome-wide distribution of histone variants and histone posttranslational modifications (PTMs). In this chapter, we describe a detailed native ChIP protocol and downstream procedures for the preparation of DNA libraries for next-generation sequencing.
Alicia, Alonso +2 more
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Chromatin Immunoprecipitation in Skeletal Myoblasts
2018Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation.
Vinay Kumar, Rao +2 more
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Chromatin immunoprecipitation in postmortem brain
Journal of Neuroscience Methods, 2006Methylation and other covalent modifications of nucleosome core histones are key regulators of chromatin structure and function, including epigenetic control of gene expression. For the human brain, however, very little is known about the regulation of histone modifications at specific genomic loci.
Huang, Hsien-Sung +3 more
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Chromatin Immunoprecipitation of Mouse Embryos
2011During prenatal development, a large number of different cell types are formed, the vast majority of which contain identical genetic material. The basis of the great variety in cell phenotype and function is the differential expression of the approximately 25,000 genes in the mammalian genome.
Anne K, Voss +4 more
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The Current State of Chromatin Immunoprecipitation
Molecular Biotechnology, 2010The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest is selectively immunoprecipitated from a chromatin preparation to determine the ...
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