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Chromatin Immunoprecipitation Assays
2004Chromatin immunoprecipitation (ChIP) is a powerful tool to study protein-DNA interaction and is widely used in many fields to study proteins associated with chromatin, such as histone and its isoforms and transcription factors, across a defined DNA domain.
Yan, Yan, Haobin, Chen, Max, Costa
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Native Chromatin Immunoprecipitation
2004Chromatin immunoprecipitation (ChIP) is a technique widely used for determining the genomic location of modified histones and other chromatin-associated factors. Here we describe the methodology we have used in our laboratory for the immunoprecipitation of chromatin isolated from cells in the absence of crosslinking.
Thorne, Alan, Myers, Fiona, Hebbes, Tim
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Chromatin Immunoprecipitation Using Microarrays
Methods in Molecular Biology, 2009Chromatin immunoprecipitation (ChIP) is a powerful procedure to investigate the interactions between proteins and DNA. ChIP-chip combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in vivo.
Mickael Durand-Dubief, Karl Ekwall
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Nanobody-Based Chromatin Immunoprecipitation
2012Chromatin immunoprecipitation (ChIP), followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq), is becoming a widely used powerful method for the analysis of the in vivo DNA-protein interactions at genomic scale.The success of ChIP largely depends on the quality of antibodies.
NGUYEN DUC, Trong +5 more
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Chromatin Immunoprecipitation and Circadian Rhythms
2022Organisms exhibit daily changes of physiology and behavior to exert homeostatic adaptations to day-night cycles. The cyclic fluctuation also takes place at transcriptional levels, giving rise to rhythmic gene expression. Central to this oscillatory transcription is the core clock machinery which constitutes a circuit of transcriptional-translational ...
Kenichiro, Kinouchi +2 more
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Chromatin Immunoprecipitation in Mammalian Cells
2009The ensemble of the genes in the mammalian genome is organized into a structure of DNA and proteins known as chromatin. The control of gene expression by the proteins that bind to chromatin regulates many cell processes, such as differentiation and proliferation.
Amy, Svotelis +2 more
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Putting chromatin immunoprecipitation into context
Journal of Cellular Biochemistry, 2009AbstractChromatin immunoprecipitation (ChIP), when paired with sequencing or arrays, has become a method of choice for the unbiased identification of genomic‐binding sites for transcription factors and epigenetic marks in various model systems. The data generated is often then interpreted by groups seeking to link these binding sites to the expression ...
Zecchini, Vincent, Mills, Ian G
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Chromatin Immunoprecipitation in Macrophages
2018Macrophages are highly polymorphic depending upon their cellular origin and their tissue environment. The different forms that a macrophage can adopt fundamentally reflect different transcription patterns. In addition, macrophages are exquisitely sensitive to a wide variety of signals coming from either infectious agents or damaged tissues. Most of the
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Chromatin Immunoprecipitation in Fission Yeast
2011A tremendous amount of information regarding the nature and regulation of heterochromatin has emerged in the past 10 years. This rapid progress is largely due to the development of techniques such as chromatin immunoprecipitation or "ChIP," which allow analysis of chromatin structure. Further technological advances such as microarray analysis and, more
Thomas A, Volpe, Jessica, Demaio
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Chromatin immunoprecipitation in postmortem brain
Journal of Neuroscience Methods, 2006Methylation and other covalent modifications of nucleosome core histones are key regulators of chromatin structure and function, including epigenetic control of gene expression. For the human brain, however, very little is known about the regulation of histone modifications at specific genomic loci.
Huang, Hsien-Sung +3 more
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