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Histone Native Chromatin Immunoprecipitation
2018Chromatin immunoprecipitation (ChIP) is becoming the standard method to study genome-wide distribution of histone variants and histone posttranslational modifications (PTMs). In this chapter, we describe a detailed native ChIP protocol and downstream procedures for the preparation of DNA libraries for next-generation sequencing.
Alicia, Alonso +2 more
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Chromatin Immunoprecipitation of Mouse Embryos
2011During prenatal development, a large number of different cell types are formed, the vast majority of which contain identical genetic material. The basis of the great variety in cell phenotype and function is the differential expression of the approximately 25,000 genes in the mammalian genome.
Anne K, Voss +4 more
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The Current State of Chromatin Immunoprecipitation
Molecular Biotechnology, 2010The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest is selectively immunoprecipitated from a chromatin preparation to determine the ...
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Chromatin Immunoprecipitation in Skeletal Myoblasts
2018Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation.
Vinay Kumar, Rao +2 more
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Chromatin RNA Immunoprecipitation (ChRIP)
2017Researchers have recently had a growing interest in understanding the functional role of long noncoding RNAs (lncRNAs) in chromatin organization. Accumulated evidence suggests lncRNAs could act as interphase molecules between chromatin and chromatin remodelers to define the epigenetic code.
Tanmoy, Mondal +2 more
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The Fast Chromatin Immunoprecipitation Method
2009The chromatin immunoprecipitation assay (ChIP assay) has greatly facilitated the recent, dramatic expansion of our knowledge of the protein-DNA interactions involved in regulating gene expression, DNA repair, and cell division. The power of the assay is that it gives a researcher the ability to not only detect a specific protein-DNA interaction in vivo
Joel, Nelson +2 more
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Chromatin Immunoprecipitation in Crops
Chromatin immunoprecipitation (ChIP) is a well-established technique that allows for the identification of genomic regions where proteins such as transcription factors, histones, or other chromatin-associated proteins bind. Moreover, deep sequencing of immunoprecipitated DNA (ChIP-seq) provides unbiased genome-wide insights into these binding sites ...openaire +2 more sources
Topoisomerase II Chromatin Immunoprecipitation
2017Chromatin immunoprecipitation is a method to isolate a protein of interest coupled to DNA following cross-linking with formaldehyde and to quantify the relative abundance or occupancy of the protein at specific genomic loci. After immunoprecipitation of protein-DNA complexes protein-DNA cross-links are reversed and the DNA is extracted. Various methods
Smith KA, Cowell IG, Austin CA
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The State-of-the-Art of Chromatin Immunoprecipitation
2009The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest is selectively immunoprecipitated from a chromatin preparation to determine the ...
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2014
Regulation of gene expression must be tightly controlled during embryonic development. A central mechanism to control gene expression is the binding of sequence-specific transcription factors to cis-regulatory elements in the genome. Chromatin immunoprecipitation (ChIP) is a widely used technique to analyze binding of transcription factors and histone ...
Amin, Shilu, Bobola, Nicoletta
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Regulation of gene expression must be tightly controlled during embryonic development. A central mechanism to control gene expression is the binding of sequence-specific transcription factors to cis-regulatory elements in the genome. Chromatin immunoprecipitation (ChIP) is a widely used technique to analyze binding of transcription factors and histone ...
Amin, Shilu, Bobola, Nicoletta
openaire +3 more sources