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Chromatin Immunoprecipitation in Crops
Chromatin immunoprecipitation (ChIP) is a well-established technique that allows for the identification of genomic regions where proteins such as transcription factors, histones, or other chromatin-associated proteins bind. Moreover, deep sequencing of immunoprecipitated DNA (ChIP-seq) provides unbiased genome-wide insights into these binding sites ...openaire +2 more sources
Chromatin Immunoprecipitation in Fission Yeast
2011A tremendous amount of information regarding the nature and regulation of heterochromatin has emerged in the past 10 years. This rapid progress is largely due to the development of techniques such as chromatin immunoprecipitation or "ChIP," which allow analysis of chromatin structure. Further technological advances such as microarray analysis and, more
Thomas A, Volpe, Jessica, Demaio
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The Fast Chromatin Immunoprecipitation Method
2009The chromatin immunoprecipitation assay (ChIP assay) has greatly facilitated the recent, dramatic expansion of our knowledge of the protein-DNA interactions involved in regulating gene expression, DNA repair, and cell division. The power of the assay is that it gives a researcher the ability to not only detect a specific protein-DNA interaction in vivo
Joel, Nelson +2 more
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Topoisomerase II Chromatin Immunoprecipitation
2017Chromatin immunoprecipitation is a method to isolate a protein of interest coupled to DNA following cross-linking with formaldehyde and to quantify the relative abundance or occupancy of the protein at specific genomic loci. After immunoprecipitation of protein-DNA complexes protein-DNA cross-links are reversed and the DNA is extracted. Various methods
Smith KA, Cowell IG, Austin CA
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The State-of-the-Art of Chromatin Immunoprecipitation
2009The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest is selectively immunoprecipitated from a chromatin preparation to determine the ...
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2014
Regulation of gene expression must be tightly controlled during embryonic development. A central mechanism to control gene expression is the binding of sequence-specific transcription factors to cis-regulatory elements in the genome. Chromatin immunoprecipitation (ChIP) is a widely used technique to analyze binding of transcription factors and histone ...
Amin, Shilu, Bobola, Nicoletta
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Regulation of gene expression must be tightly controlled during embryonic development. A central mechanism to control gene expression is the binding of sequence-specific transcription factors to cis-regulatory elements in the genome. Chromatin immunoprecipitation (ChIP) is a widely used technique to analyze binding of transcription factors and histone ...
Amin, Shilu, Bobola, Nicoletta
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Chromatin Immunoprecipitation (ChIP)
2020Chromatin immunoprecipitation or ChIP is an excellent method of investigation of the specific protein interaction and its altered forms with DNA region. These interactions have a significant role in various cellular processes such as replication, transcription, DNA damage repair, genome stability, gene regulation and segregation at mitosis.
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Chromatin Immunoprecipitation in Yeast
Cold Spring Harbor Protocols, 2006David C, Amberg +2 more
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