Results 41 to 50 of about 357,335 (290)

Dynamic interaction of HMGA1a proteins with chromatin [PDF]

Journal of Cell Science, 2004
High-mobility-group proteins A1 (HMGA1; previously named HMGI/Y) function as architectural chromatin-binding proteins and are involved in the transcriptional regulation of several genes. We have used cells expressing proteins fused to green fluorescent protein (GFP) and fluorescence recovery after photobleaching (FRAP) to analyze the distribution and ...
Monika, Harrer, Hardi, Lührs, Michael, Bustin, Ulrich, Scheer, Robert, Hock   +4 more
openaire   +2 more sources

HiCoP, a simple and robust method for detecting interactions of regulatory regions

Epigenetics & Chromatin, 2020
Background Chromatin physical interactions provide essential information for understanding the regulation of cis-elements like enhancers, promoters, and insulators in cell development and differentiation. The Hi-C assay is a technique detecting chromatin
Yan Zhang, Zhaoqiang Li, Shasha Bian, Hao Zhao, Delong Feng, Yanhong Chen, Yuhe Hou, Qifa Liu, Bingtao Hao   +8 more
doaj   +1 more source

DNA curtains to visualize chromatin interactions. [PDF]

Methods, 2023
Recent advances in single molecule imaging have allowed the evolution of biochemical techniques that directly visualize protein-DNA interactions in real-time. These techniques rely on diffraction limited total internal reflection microscopy (TIRFM), and have significantly improved our understanding of RNA transcription, DNA replication, homologous ...
Woodhouse M, Brooks Crickard J.
europepmc   +3 more sources

Papillomavirus interaction with cellular chromatin [PDF]

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2010
High-risk human papillomavirus (HPV) infection is the primary risk factor for cervical cancer. HPVs establish persistent infection by maintaining their genomes as extrachromosomal elements (episomes) that replicate along with host DNA in infected cells.
openaire   +2 more sources

Epigenomes in Cardiovascular Disease. [PDF]

, 2018
If unifying principles could be revealed for how the same genome encodes different eukaryotic cells and for how genetic variability and environmental input are integrated to impact cardiovascular health, grand challenges in basic cell biology and ...
McKinsey TA, Morimoto T, Poller W, Turner BM, Wang Y   +4 more
core   +1 more source

Microscale Thermophoresis Analysis of Chromatin Interactions [PDF]

, 2018
Architectural DNA-binding proteins are key to the organization and compaction of genomic DNA inside cells. The activity of architectural proteins is often subject to further modulation and regulation through the interaction with a diverse array of other protein factors.
Corbeski, I., Horn, V., Valk, R.A.van der, le Paige, U., Dame, R.T., Ingen, H.van   +5 more
openaire   +7 more sources

3C-Based Chromatin Interaction Analyses [PDF]

Cold Spring Harbor Protocols, 2018
This introduction presents a molecular approach that uses formaldehyde cross-linking to investigate genome structure and function—chromosome conformation capture (3C). This approach allows us to determine the spatial proximity of distant functional genomic sites (by looping).
Kim, Tae Hoon, Dekker, Job
openaire   +3 more sources

Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement. [PDF]

, 2019
Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin ...
Afonine, Afonine, Allen, Angus-Hill, Ashkenazy, Ashkenazy, Asturias, Badis, Buchan, Buchan, Cairns, Cairns, Campsteijn, Chaban, Chambers, Chatterjee, Clapier, Clapier, Côté, Da, Dechassa, Duan, Dutta, Dürr, El-Gebali, Emsley, Erkina, Filippakopoulos, Goddard, Han, Jones, Jones, Kadoch, Kasten, Klein-Brill, Krietenstein, Kuryan, Leschziner, Leschziner, Li, Liu, Lopéz-Blanco, Mashtalir, Morin, Nagai, Nakane, Ng, Patel, Pettersen, Puig, Saha, Sammak, Schorb, Schubert, Sen, Shim, Sievers, Skiniotis, Smith, Suloway, Söding, Tan, Tsunaka, Ungar, Valencia, Wagner, Wang, Wilson, Xia, Yan, Yang, Ye, Yen, Zhang, Zhang, Zhang, Zheng, Zivanov, Zivanov, Zukin   +79 more
core   +3 more sources

Shigella generates distinct IAM subpopulations during epithelial cell invasion to promote efficient intracellular niche formation

European Journal of Cell Biology
The facultative intracellular pathogen Shigella flexneri invades non-phagocytic epithelial gut cells. Through a syringe-like apparatus called type 3 secretion system, it injects effector proteins into the host cell triggering actin rearrangements leading
Lisa Sanchez, Arthur Lensen, Michael G. Connor, Mélanie Hamon, Jost Enninga, Camila Valenzuela   +5 more
doaj   +1 more source

3D organization of regulatory elements for transcriptional regulation in Arabidopsis

Genome Biology, 2023
Background Although spatial organization of compartments and topologically associating domains at large scale is relatively well studied, the spatial organization of regulatory elements at fine scale is poorly understood in plants.
Li Deng, Qiangwei Zhou, Jie Zhou, Qing Zhang, Zhibo Jia, Guangfeng Zhu, Sheng Cheng, Lulu Cheng, Caijun Yin, Chao Yang, Jinxiong Shen, Junwei Nie, Jian-Kang Zhu, Guoliang Li, Lun Zhao   +14 more
doaj   +1 more source