Results 91 to 100 of about 519,147 (169)

pDNA capture using grafted adsorbents [PDF]

, 2018
BACKGROUND: ‘Expanded’ composite materials are of interest as an alternative, or as a supplement, to packed-bed chromatography during bioproduct recovery and purification.
Bencina, Bibi, Branovic, Cardoso, Colote, Diogo, Eon-Duval, Franco-Medrano, Gavara, Li, Lozinsky, Podgornik, Potter, Prazeres, Prazeres, Silva-Santos, Singh, Singh, Singh, Singh, Smrekar, Teeters, Tiainen, Urthaler, Urthaler   +24 more
core   +1 more source

Removal of dsRNA byproducts using affinity chromatography

Molecular Therapy: Nucleic Acids
Double-stranded RNA (dsRNA) molecules are immunogenic byproducts of in vitro transcription of single-stranded RNA (ssRNA). Removal of dsRNA from ssRNA is difficult because the byproducts have similar sizes, sequences, and charges to the desired ssRNA ...
Nathaniel E. Clark, Mateusz Kozarski, Sinem Demirel Asci, Jurgen Van den Heuvel, Matt R. Schraut, Roger A. Winters, Kelley Kearns, Thomas C. Scanlon, Senne Dillen   +8 more
doaj  

Christhin: Quantitative Analysis of Thin Layer Chromatography [PDF]

arXiv, 2012
Manual for Christhin 0.1.36 Christhin (Chromatography Riser Thin) is software developed for the quantitative analysis of data obtained from thin-layer chromatographic techniques (TLC). Once installed on your computer, the program is very easy to use, and provides data quickly and accurately.
arxiv  

Synthesis and Application of Dye-Ligand Affinity Adsorbents [PDF]

, 2014
Dye-ligand affinity chromatography is a widely used technique in protein purification. The utility of the reactive dyes as affinity ligands results from their unique chemistry, which confers wide specificity towards a large number of proteins.
A Berg, A Doğan, A Maltezos, E Chronopoulou, F Kuralay, M Andac, MM Bradford, NE Katsos, NE Labrou, NE Labrou, NE Labrou, NE Labrou, NE Labrou, NE Labrou, NM Lindner, PM Boyer, RK Scopes, SJ Burton, SJ Burton, T Eisele, YD Clonis   +20 more
core   +1 more source

Sphere Packing on a Quantum Computer for Chromatography Modeling [PDF]

arXiv
Column chromatography is an important process in downstream biopharmaceutical manufacturing that enables high-selectivity separation of proteins through various modalities, such as affinity, ion exchange, hydrophobic interactions, or a combination of the aforementioned modes.
arxiv  

Quantifying the Differences in Binding Affinity of Reduced Glutathione for Glutathione S-Transferase at pH 6.5 and 8.5 Using Isothermal Titration Calorimetry

, 2018
The binding affinity between an enzyme and its substrate is often dependent on the pH of the local environment. Knowing the pH at which reduced glutathione (GSH) binds with the highest affinity to the enzyme glutathione S-transferase (GST) is useful for ...
Gary, Ronald K., Renshaw, Clay P., Jr.
core  

Concanavalin A-Binding Enzymes of Crotalus scutulatus scutulatus Venom [PDF]

, 1985
Crotalus scutulatus scutulatus crude venom was separated into two fractions by Concanavalin A Sepharose 4B affinity chromatography. The proteins binding to Con A exhibited phosphomonoesterase (orthophosphoric monoester phosphohydrolase EC 3.1.3.2 ...
Childs, C. K., Hinson, Michael W., Johnson, Bob D., Sifford, Dewey H.   +3 more
core   +4 more sources

Purification of a Protein Exhibiting Isoleucine 2-epimerase Activity from Lactobacillus otakiensis JCM 15040

Bio-Protocol, 2015
Prominent accumulation of D-leucine, D-allo-isoleucine and D-valine was observed in the culture medium of the heterofermentative bacterial species, Lactobacillus otakiensis (L. otakiensis) JCM 15040. The racemase enzyme that resulted in this accumulation,
Yuta Mutaguchi, Toshihisa Ohshima
doaj   +1 more source

A general strategy for discovery of inhibitors and activators of RING and U-box E3 ligases with ubiquitin variants [PDF]

, 2017
RING and U-box E3 ubiquitin ligases regulate diverse eukaryotic processes and have been implicated in numerous diseases, but targeting these enzymes remains a major challenge.
Ahmed, Syed Feroj, Buetow, Lori, Gabrielsen, Mads, Huang, Danny T., Nakasone, Mark A., Sibbet, Gary J., Sidhu, Sachdev S., Smith, Brian O., Zhang, Wei   +8 more
core   +2 more sources

A High Accuracy Symplectic Scheme for Advection Diffusion Reaction Models in Bioseparation [PDF]

arXiv
We analyze an advection-diffusion-reaction problem with non-homogeneous boundary conditions that models the chromatography process, a vital stage in bioseparation. We prove stability and error estimates for both constant and affine adsorption, using the symplectic one-step implicit midpoint method for time discretization and finite elements for spatial
arxiv