Results 101 to 110 of about 297,526 (265)

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine
Lentiviral vectors (LVVs) are emerging as an enabling tool in gene and cell therapies, yet the toolkit for purifying them at scale is still immature. A pivoting moment in LVV isolation technology was marked by the introduction of affinity ligands for ...
Eduardo Barbieri, Gina N. Mollica, Sobhana A. Sripada, Shrirarjun Shastry, Yuxuan Wu, Arianna Minzoni, Will Smith, Elena Wuestenhagen, Annika Aldinger, Heiner Graalfs, Michael S. Crapanzano, Oliver Rammo, Michael M. Schulte, Michael A. Daniele, Stefano Menegatti   +14 more
doaj   +1 more source

Dual‐Functional Polyphosphoesters for Gene Delivery: Synergistic Effects of Guanidinium and Hydrophobic Side Chains in Degradable Polymers

Advanced Healthcare Materials, EarlyView.
This study presents guanidinium‐ and indole‐functionalized polyphosphoesters as degradable, non‐viral gene delivery vectors. Through precise tuning of charge density and hydrophobicity, these polymers form stable polyplexes with low toxicity. Remarkably, minor structural changes yield up to 200‐fold differences in transfection efficiency, highlighting ...
Markus Kötzsche, Andreas Dzierza, Jan Egger, Timo Rheinberger, Christin Weilandt, Frederik R. Wurm, Ivo Nischang, Dagmar Fischer, Kalina Peneva   +8 more
wiley   +1 more source

Polyclonal Antibody Production against Mouse Purified IgG2a towards Use in Basic Research

Research in Molecular Medicine, 2016
Background: The ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis.
Zahra Moradi nebrin, Jafar Majidi, Leili Aghebati Maleki, Tohid Kazemi, Jalal Abdolalizadeh, Somaieh Dadashi, Sadeg Eyvazi, Majid Ahmadi, Naiemeh Majidi zolbanin   +8 more
doaj  

Amine‐Functionalized Chitosan/dECM Composite Promotes Cutaneous Regeneration and Hair Follicle Activation by Regulating Oxidative Stress and Inflammation

Advanced Healthcare Materials, EarlyView.
An amine‐functionalized chitosan and hydrolyzed dECM composite promotes cutaneous regeneration by modulating oxidative stress and inflammatory pathways. The material accelerates wound closure and stimulates hair follicle activation in UV‐damaged cutaneous tissue.
Yu‐Jin Kim, Seokheon Hong, Jae Hwi Lee, Jongmin Lee, Jae Jun Kang, Jiseon Bae, Jino Kim, Dong‐Hwee Kim, Jin Yoo, Mi Sun Cha, Su Hee Kim, Youngmee Jung   +11 more
wiley   +1 more source

Low‐Temperature Fabrication of Thymosin β4‐Loaded Soluble Microneedles to Promote Wound Healing by Specific Binding to Downregulated Immune Regulators Vsig4 and IL22rɑ2

Advanced Healthcare Materials, EarlyView.
Schematic diagram depicting the fabrication and application of thymosin β4 (Tβ4)‐loaded microneedle patches for wound treatment. The Tβ4 was loaded into chitosan (CS) and sucrose MNs under mild conditions (4°C, 65% relative humidity). The Tβ4 MN patch specifically binds to the downregulated immune regulators Vsig4 and IL22rα2, thereby accelerating ...
Shilong He, Mei Yuan, Changkang Feng, Yixuan Zhang, Jinjing Che   +4 more
wiley   +1 more source

affinity chromatography

, 2014
Citation: 'affinity chromatography' in the IUPAC Compendium of Chemical Terminology, 3rd ed.; International Union of Pure and Applied Chemistry; 2006. Online version 3.0.1, 2019. 10.1351/goldbook.A00177 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
openaire   +1 more source

Engineered ETS1‐Nanoconjugate Restores Immune Homeostasis through Dual Immune‐Vascular Modulation in Relapsing and Progressive Multiple Sclerosis

Advanced Healthcare Materials, EarlyView.
The biomimetic nanoplatform IMNP (ETS1 pDNA/PBAE@ITP‐MM) undergoes targeted disassembly at inflammatory vascular sites to release the ETS1 plasmid (pETS1). This release initiates a cascade of effects that inhibit pathogenic pathways and support immune homeostasis. (Abbreviations: EndMT, endothelial‐to‐mesenchymal transition; EC, endothelial cell; TC, T
Feng Zhang, Si‐Bo Yang, Yan Luo, Xiao‐Di Sun, Bo‐Hao Chang, Da‐Qiang Zhou, Jun‐Jie Jiang, Ya‐Nan Li, Jie‐Hong Wu, Hui‐Juan Jin, Hang Yang, Hang Hu, Yi‐Fan Zhou, Bo Hu   +13 more
wiley   +1 more source

PURIFICATION AND ANALYSIS OF CARBOHYDRATE CLUSTER FROM ERYTHROPOIETIN RECOMBINANT EXPRESSION RESULT ON THE LEAVEN SYSTEM OF Pichia pastoris

Makara Seri Sains, 2011
For clinical purposes, pure protein and identification of carbohydrate structure from erythropoietin recombinant are needed. Purification was done with His-Trap affinity chromatography method and continued with gel filtration chromatography column to get
Adi Santoso, Andri Wardiana
doaj  

Engineered Protein‐Based Ionic Conductors for Sustainable Energy Storage Applications

Advanced Materials, EarlyView.
Rational incorporation of charged residues into an engineered, self‐assembling protein scaffold yields solid‐state protein films with outstanding ionic conductivity. Salt‐doping further enhances conductivity, an effect amplified in the engineered variants. These properties enable the material integration into an efficient supercapacitor.
Juan David Cortés‐Ossa, Paolo Blesio, Marcial Fernandez‐Castro, Lisa Almonte, Maxence Fernandez, Mantas Liutkus, Perumal Pandurangan, Carlos Sabater, Aitor Villaverde, Manuel Melle‐Franco, Nurit Ashkenasy, Felipe Jiménez‐Ángeles, M. Carmen Morant‐Miñana, M. Reyes Calvo, Aitziber L. Cortajarena   +14 more
wiley   +1 more source

Rainbow Photonic Crystals: Self‐Assembly of Carbohydrate Bottlebrushes Block Copolymers

Advanced Materials, EarlyView.
Full‐color reflective 1D photonic crystals, capable of a tunable, responsive, and reversible color with high repeatability, are created from the self‐assembly of carbohydrate‐based brush block copolymers (BR01‐04). Abstract Tunable and responsive photonic crystal systems are the subject of great research interest owing to their ability to actively ...
Hong Li, Muhammad Mumtaz, Takuya Isono, Toshifumi Satoh, Cheng‐Liang Liu, Wen‐Chang Chen, Redouane Borsali   +6 more
wiley   +1 more source