Results 21 to 30 of about 297,526 (265)

Non-targeted characterization of attributes affecting antibody-FcγRIIIa V158 (CD16a) binding via online affinity chromatography-mass spectrometry

mAbs, 2022
Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall, Thomas M. Dillon, Kevin Kalenian, Rupa Padaki, Scott Kuhns, David J. Semin, Pavel V. Bondarenko   +6 more
doaj   +1 more source

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Biomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography

Pharmaceutics, 2022
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes, Magnus Bergman, Anita Solbrand, Cristina Peixoto, Manuel J. T. Carrondo, Ricardo J. S. Silva   +5 more
doaj   +1 more source

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

Química Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki, Cassiana Mazzer, Rúbia Pazzetto, Graciette Matioli   +3 more
doaj   +1 more source

Rapid determination of serum albumin partition coefficients using affinity chromatography

Environmental Advances, 2022
Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target ...
Nadin Ulrich, Alexander Böhme
doaj   +1 more source

Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography

Sensors, 2017
l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne, Stefanie Wedepohl, Jens Dernedde   +2 more
doaj   +1 more source

Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

Czech Journal of Food Sciences, 2014
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco, Flávio Faria De Moraes, Natalia Sozza Bernardi, Mary Helen Palmuti Braga Vettori, Rubens Monti, Jonas Contiero   +5 more
doaj   +1 more source

High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography

Molecular Therapy: Methods & Clinical Development, 2023
The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients.
Michael Florea, Fotini Nicolaou, Simon Pacouret, Eric M. Zinn, Julio Sanmiguel, Eva Andres-Mateos, Carmen Unzu, Amy J. Wagers, Luk H. Vandenberghe   +8 more
doaj   +1 more source