Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo. [PDF]
, 1989 We have developed affinity chromatography methods for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts and have used them to analyze the cytoskeleton of the early Drosophila embryo.
Alberts, BM, Field, CM, Kellogg, DR
core
Protein Purification Using PDZ Affinity Chromatography [PDF]
, 2015 PDZ domains function in nature as protein-binding domains within scaffold and membrane-associated proteins. They comprise approximately 90 residues and undergo specific, high-affinity interactions with complementary C-terminal peptide sequences, other ...
Kennedy, Mary B., Walkup, Ward G., IV
core +1 more source
HD gas analysis with Gas Chromatography and Quadrupole Mass Spectrometer [PDF]
, 2011 A gas analyzer system has been developed to analyze Hydrogen-Deuteride (HD) gas for producing frozen-spin polarized HD targets, which are used for hadron photoproduction experiments at SPring-8. Small amounts of ortho-H$_{2}$ and para-D$_{2}$ gas mixtures ($\sim$0.01%) in the purified HD gas are a key to realize a frozen-spin polarized target. In order
arxiv +1 more source
Minimal Information About Sample Preparation for Phosphoproteomics [PDF]
, 2009 This guideline describes parameters and conditions involved in phosphopeptide sample preparation. It covers from the description and preparation of the cells and tissues to the fractionation and specific enrichment of phosphopeptides for MS analysis. The
David Ovelleiro +3 more
core +2 more sources
Zhongguo shuxue zazhi[Objective] To determine the feasibility of preparing plasminogen (Pg) with Fraction Ⅲ precipitation (hereinafter referred to as FⅢ-P) from low-temperature ethanol process by full chromatography (hereinafter referred to as FⅢ-P process). [Methods] The FⅢ-
ZHANG Jin +9 more
doaj +1 more source
High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography
Molecular Therapy: Methods & Clinical Development, 2023 The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients.
Michael Florea +8 more
doaj
Determination of Trace Moisture Content in Dimethylacetamide by Gas Chromatography [PDF]
arXiv, 2018 Dimethylacetamide is used as a solvent in polymer processing and moisture in DMA affects the morphology and mechanical properties of the polymer products. This paper describes a method for determination of trace moisture content in DMA by acetylene production - gas chromatography. In the condition of 30 centigrade and ultrasonic dispersion, moisture in
arxiv
Deuterated nucleotides as chemical probes of RNA structure: a detailed protocol for the enzymatic synthesis of a complete set of nucleotides specifically deuterated at ribose carbons [PDF]
, 2015 We describe here a detailed protocol for the synthesis of ribonucleotides specifically deuterated at each ribose carbon atom. We synthesized 20 specifically deuterated ribonucleotides: ATP, CTP, GTP, and UTP, each of which contained one of five ...
Azad, Robert +2 more
core +3 more sources
Brazilian Archives of Biology and Technology, 2011 Lectins are proteins or glycoproteins able to bind, specifically and reversibly carbohydrates and glycoconjugates. Considering this ability, the utilization of Caesalpinia pulcherrima seeds polysaccharides as an affinity matrix was tested.
Renata Chastinet Braga +6 more
doaj +1 more source
Kinetic formulation of a 2 $\times$ 2 hyperbolic system arising in gas chromatography [PDF]
arXiv, 2017 The PSA system commonly used in the context of gas-solid chromatography is reformulated as a single kinetic equation using an additional kinetic variable. A kinetic numerical scheme is built from this new formulation and its behavior is tested on solving the Riemann problem in different configurations leading to single or composite waves.
arxiv