Determination of carbohydrates in infant milk powders by ultra-performance liquid chromatography with evaporative light scattering detector and BEH HILIC column [PDF]
The possibility of carbohydrate separation in BEH HILIC (Ethylene Bridged Hybride, Hydrophilic Interaction Liquid Chromatography) column was studied by ultra-performance liquid chromatography (UPLC) with evaporative light scattering detector (ELSD) and mobile phase containing amine compounds as modifiers.
arxiv
Purification of the food-borne carcinogens 2-amino-3-methylimidazo [4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline in heated meat products by immunoaffinity chromatography [PDF]
A rapid and simple scheme has been developed for the isolation and purification of two of the major mutagenic heterocyclic amines formed in heated beef products by affinity chromatography using monoclonal antibodies which recognize 2-amino-3 ...
Aeschbacher, Hans Ulrich+6 more
core
Cyclic nucleotide specificity of the activator and catalytic sites of a cGMP-stimulated cGMP phosphodiesterase from Dictyostelium discoideum [PDF]
The cellular slime mold Dictyostelium discoideum has an intracellular phosphodiesterase which specifically hydrolyzes cGMP. The enzyme is activated by low cGMP concentrations, and is involved in the reduction of chemoattractant-mediated elevations of ...
Baraniak, Janina,+8 more
core +2 more sources
Characterization of Tyrosinase Inhibitors in Dryopteris crassirhizoma Rhizome Using a Combination of High-Speed Counter-Current Chromatography, Affinity-Based Ultrafiltration, and Liquid Chromatography-Tandem Mass Spectrometry. [PDF]
Wang Z, Wang N, Han D, Yan H.
europepmc +1 more source
The refolding of recombinant human liver methylmalonyl-CoA mutase from inclusion bodies produced in Escherichia coli : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]
Human methylmalonyl-CoA mutase (hMCM) is an adenosylcobalamin-dependent enzyme that catalyses the structural rearrangement of (R)-methylmalonyl-CoA to succinyl-CoA as pan of the catabolism of the branched chain amino acids valine, leucine and isoleucine,
Hayes, Michelle Marie
core
Successive aqueous (10 ºC, 25 ºC and 80 ºC) and alkaline (1M NaOH; 25 ºC) extractions of milled Parkinsonia aculeata endosperms gave rise to four galactomannan fractions. These extractions furnished viscous galactomannans with Man:Gal ratios ranging from
Ivone Garros-Rosa+4 more
doaj +1 more source
Isolation and Characterization of Peanut (Arachis hypogaea) Lectin [PDF]
Plant lectins which belong to a class of glycoprotein have a wide spectrum of biological significance. In this thesis, the isolation and characterization of the lectins (AHL and PNA) from the peanut (Arachis hypogaea) have been reported.
Das, Chandan Kanta
core
Lapatinib-binding protein kinases in the African trypanosome: identification of cellular targets for kinase-directed chemical scaffolds. [PDF]
Human African trypanosomiasis is caused by the eukaryotic microbe Trypanosoma brucei. To discover new drugs against the disease, one may use drugs in the clinic for other indications whose chemical scaffolds can be optimized via a medicinal chemistry ...
Abagyan, Ruben+7 more
core +2 more sources
Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions
Ligand-target interactions play a central role in drug discovery processes because these interactions are crucial in biological systems. Small molecules-proteins interactions can regulate and modulate protein function and activity through conformational ...
Marcela Cristina de Moraes+2 more
doaj +1 more source
Some new well-posedness results for continuity and transport equations, and applications to the chromatography system [PDF]
We obtain various new well-posedness results for continuity and transport equations, among them an existence and uniqueness theorem (in the class of strongly continuous solutions) in the case of nearly incompressible vector fields, possibly having a blow-up of the BV norm at the initial time. We apply these results (valid in any space dimension) to the
arxiv