Results 1 to 10 of about 370,485 (298)

Ion-exchange chromatography purification of extracellular vesicles

open access: yesBioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović   +4 more
doaj   +2 more sources

Antibody Aggregate Removal by Multimodal Chromatography [PDF]

open access: yesMolecules
The growing demand for therapeutic monoclonal antibodies (mAbs) has heightened the need for efficient and scalable purification strategies. A major challenge in downstream processing is the removal of antibody aggregates, which can compromise drug safety,
Veronika Rupčíková   +3 more
doaj   +2 more sources

Consecutive Affinity and Ion-Exchange Chromatography for AAV9 Vectors Purification [PDF]

open access: yesBiomedicines
Background: Irrespective of the rapid development of AAV-based gene therapy, the production of clinical-grade vectors has a bottleneck resulting from product-related impurities such as empty and partially filled capsids, which lack a functional ...
Ozgun Firat Duzenli, George Aslanidi
doaj   +2 more sources

Effect of ion-exchange chromatography on removal of fibronectin in von Willebrand factor

open access: yesZhongguo shuxue zazhi, 2023
Objective To study the removal effect of fibronectin(Fn) from von willebrand factor(vWF) by ion-exchange chromatography through processing human coagulation factor Ⅷ chromatographic washing products, in order to select a method that can effectively ...
Zheng ZHOU   +4 more
doaj   +1 more source

The Role of Vibrio cholerae Haemagglutinin Protease (HAP) in Extra-Intestinal Infection [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2016
Introduction: Based on the diversity of surface O antigen Vibrio cholerae can be classified into 206 serogroups. Vibrio cholerae is the causative agent of cholera and extra intestinal infections like, septicemia, wound infection and haemorrhagic ...
Amit Ghosh, Hemanta Koley, Amit Pal
doaj   +1 more source

The role of lactic acid adsorption by ion exchange chromatography. [PDF]

open access: yesPLoS ONE, 2010
BACKGROUND: The polyacrylic resin Amberlite IRA-67 is a promising adsorbent for lactic acid extraction from aqueous solution, but little systematic research has been devoted to the separation efficiency of lactic acid under different operating conditions.
Qiang Gao   +7 more
doaj   +1 more source

Simultaneous determination of chloride, bromide and iodide in foodstuffs by low pressure ion-exchange chromatography with visible light detection

open access: yesCzech Journal of Food Sciences, 2011
An ion-exchange chromatography method with visible light detection was developed for the simultaneous determination of chloride, bromide, and iodide in foodstuffs.
Lingyun Yu   +4 more
doaj   +1 more source

Study on separation and purification process of C1 esterase inhibitor

open access: yesZhongguo shuxue zazhi, 2022
Objective To study the technology of separating and purifying C1 esterase inhibitor (C1-INH) by using the waste washing liquid as raw materia during the preparation of human prothrombin complex (PCC) l.
Jiabin XU   +4 more
doaj   +1 more source

Antimicrobial activity of endophytic fungi Kabatiella caulivora Var. A from Alyxia reinwardtii Bl.

open access: yesJournal of Biological Researches, 2012
Isolation and characterization of β-1,3-endoglucanase from cabbage (Brassica oleracea var. capitata L.) have been done. It showed 40° C of optimum temperature, and optimum pH is 7.
Y. Sri Wulan Manuhara
doaj   +1 more source

Water, electricity and ion exchange; how Hamish Small sustained the evolution of ion chromatography

open access: yesHeliyon, 2021
Since the development of synthetic ion exchange resins in 1935, the electrochemical properties of these unique materials have intrigued researchers. In 1951, Hamish Small was introduced to synthetic ion exchange resin when he studied the electromigration
John M. Riviello
doaj  

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