Results 111 to 120 of about 125,999 (289)

Simulation model for overloaded monoclonal antibody variants separations in ion-exchange chromatography

, 2012
A model was developed for the design of a monoclonal antibody charge variants separation process based on ion-exchange chromatography. In order to account for a broad range of operating conditions in the simulations, an explicit pH and salt concentration
B. Guélat, MORBIDELLI, MASSIMO, G. Ströhlein, M. Lattuada, P. Valax, L. Delegrange   +5 more
core   +1 more source

ZDHHC9‐Mediated Palmitoylation of ACSL4 Drives Ferroptosis in Diabetes Mellitus–Induced Erectile Dysfunction

Advanced Science, EarlyView.
ABSTRACT Diabetes mellitus‐induced erectile dysfunction (DMED) is a highly prevalent complication among diabetic patients; however, its underlying pathogenic mechanisms remain incompletely understood. Metabolic disorder is a hallmark of diabetes, yet its precise contribution to DMED progression is not well defined.
Wanyang Guo, Ming Xiao, Mengjun Huang, Dongzi Peng, Ruijiang Zeng, Ruilin Liu, Yuanqiao Zhao, Zhihan Ouyang, Yulong Hong, Zexian Ding, Zhuo Xing, Hao Su, Jinxiang Wang, Wenjun Mao, Xin Jin   +14 more
wiley   +1 more source

Gut Lactate Boosts Ruminococcus via Histone Lactylation to Mediate Time‐Restricted Feeding Protection in Crohn's Disease

Advanced Science, EarlyView.
TRF enriches Ruminococcus, a bacterial genus producing SCFAs, and activates the epithelial HIF‐1α signaling pathway. This mechanism protects the colonic mucosa from inflammatory insults in colitis models. Mechanistically, gut lactate production during starvation and refeeding mediates H4K12la, which increases SLC9A3 expression and creates an acidic gut
Linwen Huang, Huishi Tan, Senhui Weng, Yuntao Liu, Lingxu Song, Shaoyu Cheng, Zelong Lin, Jiawei Chen, Fei Tan, Jun Wang, Jinke Huang, Linkun Cai, Jiwei Chai, Cailing Zhong, Yanqiang Shi, Wendi Zhang, Haiyan Zhang, Chongyang Huang   +17 more
wiley   +1 more source

Tetrastigma Hemsleyanum Polysaccharide Suppresses Triple‐Negative Breast Cancer by Disrupting the Hippo‐YAP/TEAD4‐PDIA4 Axis and Endoplasmic Reticulum Stress Adaptation

Advanced Science, EarlyView.
ABSTRACT Triple‐negative breast cancer (TNBC) exhibits addiction to chronic endoplasmic reticulum (ER) stress, which sustains an aggressive metastatic phenotype through activation of the unfolded protein response (UPR). Here, we identify a previously unrecognized “ER‐stress addiction” axis in which the Hippo pathway effector TEAD4 directly ...
Yini Shang, Wentao Si, Youxue Zhang, Jialin Liu, Huaixi Zhang, Yafei Li, Hongmeng Su, Wei Jiang, Zhishan Ding, Lihong Wang   +9 more
wiley   +1 more source

Using computer simulation to assist in the robustness analysis of an ion-exchange chromatography step

, 2005
This paper presents a methodology to gain process knowledge and assist in the robustness analysis of an ion-exchange step in a protein purification process using a model-based approach.
Axelsson, J P, Zacchi, Guido, Jakobsson, Niklas, Nilsson, Bernt, Jakobsson, Niklas,, Nilsson, Bernt,, Karlsson, David, Lund University., Zacchi, Guido,, Karlsson, David,   +9 more
core   +1 more source

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

Advanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green, Michael W. Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon‐Tamarit, Rory L. Arrowsmith, Charareh Pourzand, Gabriele Kociock‐Kohn, Nicholas H. Rees, Jonathan R. Dilworth, Stephen Faulkner, Stanley W. Botchway, David Gurevich, Sofia I. Pascu   +15 more
wiley   +1 more source

Advantages of ion-exchange chromatography for oligonucleotide analysis

, 2011
The rapid development of therapeutic oligonucleotides (ONs) has created a need for in-depth characterization of ONs, beyond previous requirements. The natural migration to LC–MS requires the use of chromatography with MS-compatible eluents to introduce ...
Ken Cook, Jim Thayer
core   +1 more source

Quantitative Mass Spectrometry‐Based Biodistribution of Monoclonal Antibodies: An Alternative to Radio‐Biodistribution

Advanced Science, EarlyView.
Monoclonal antibodies are powerful anticancer therapeutics targeting tumor‐associated antigens. Mass spectrometry enables precise and accurate quantification of mAbs in tumor plasma and healthy organs. Unlike ligand binding assay and radioactivity, mass spectrometry does not suffer from matrix effects and labelling artefacts while facilitating method ...
Domenico Ravazza, Sheila Dakhel Plaza, Samuele Cazzamalli, Andrea Ciamarone, Andrea Galbiati, Riccardo Stucchi, Frederik Peissert, Emanuele Puca, Dario Neri, Ettore Gilardoni   +9 more
wiley   +1 more source

Studies in ion exchange chromatography

, 1988
This dissertation investigates three major problems in IC, ranging from the practical to the theoretical. The first is of interest to the field of quantitative analysis.
Polite, Lee Nicholas
core  

A multifunctional dual membrane electrodialytic eluent generator for capillary ion chromatography

, 2009
A multifunctional electrodialytic generator (EDG) for capillary ion chromatography (CIC) is described. The same device can generate acidic, basic or saline eluents.
Liang, Xinmiao, 梁鑫淼, Zhang, Feifang, Yang, Bingcheng, Dasgupta, Purnendu K.   +4 more
core   +1 more source