Results 11 to 20 of about 370,485 (298)

Coupling Multi Angle Light Scattering to Ion Exchange chromatography (IEX-MALS) for protein characterization

Scientific Reports, 2018
Multi-angle light scattering coupled with size exclusion chromatography (SEC-MALS) is a standard and common approach for characterizing protein mass, overall shape, aggregation, oligomerization, interactions and purity.
Hadar Amartely, O. Avraham, A. Friedler, O. Livnah, M. Lebendiker   +4 more
semanticscholar   +1 more source

Silent hemoglobin variant during capillary electrophoresis: A case report

Journal of Diabetes Investigation, 2020
Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes.
Yanping Yuan, Xianghai Zhou, Leili Gao, Qian Ren, Linong Ji   +4 more
doaj   +1 more source

Dendrimer Anion-Exchange Stationary Phase for Separation of Oligonucleotides

Molecules, 2022
Oligonucleotides are used in many research areas. Thus, there is a need for their successful separation methods. Ion-exchange chromatography is the most popular separation technique, but it has limitations for these compounds.
Sylwia Studzińska, Szymon Bocian, Anna Kilanowska, Bogusław Buszewski   +3 more
doaj   +1 more source

Separation of Natural Collagen Crosslinks Using Buffer and Ion-pairing Agent Free Solvents on Silica Hydride Column for Mass Spectrometry Detection

Bio-Protocol, 2019
In this protocol we describe the separation of collagen crosslinks in biological tissues and samples including skin, tendon, cartilage, bone and urine. The existing methods use either cation exchange chromatography followed by post-column derivatization ...
Rafea Naffa, Joseph Pesek
doaj   +1 more source

Ion-Exclusion/Cation-Exchange Chromatography Using Dual-Ion-Exchange Groups for Simultaneous Determination of Inorganic Ionic Nutrients in Fertilizer Solution Samples for the Management of Hydroponic Culture

Agronomy, 2021
In this study, ion-exclusion/cation-exchange chromatography (IEC/CEC) using dual-ion-exchange groups (carboxy and sulfo groups) for the simultaneous determination of anions (SO42−, Cl−, NO3−, and HPO42−) and cations (Na+, NH4+, K+, Mg2+, and Ca2+) was ...
Daisuke Kozaki, Yuki Sago, Taku Fujiwara, Masanobu Mori, Chihiro Kubono, Tougo Koga, Yuta Mitsui, Tomotaka Tachibana   +7 more
doaj   +1 more source

Separation of therapeutic oligonucleotides using ion-pair reversed-phase chromatography based on fundamental separation science

Journal of Chromatography Open, 2023
In recent decades, there has been a trend toward using larger biological molecules as new active pharmaceutical ingredients (APIs) instead of the classical small organic API molecules.
Torgny Fornstedt, Martin Enmark
doaj  

Chromatographic separation of mannitol from mixtures of other carbohydrates in aqueous solutions

Czech Journal of Food Sciences, 2021
The isolation of mannitol from natural sources, e.g. from plant extracts or broths, requires considerable time and effort. The separation of mannitol from aqueous solutions containing also glucose, fructose, and sucrose was tested using discontinuous ...
Simona Gillarová, Svatopluk Henke, Tomáš Svoboda, Pavel Kadlec, Andrea Hinková, Zdeněk Bubník, Vladimír Pour, Marcela Sluková   +7 more
doaj   +1 more source

Total plasma homocysteine measurement: Evaluation of the Abbott immunoassay, comparison with the JEOL ion exchange chromatography and investigation of its clinical utility

Practical Laboratory Medicine, 2022
Objectives: Homocysteine is an intermediary amino acid formed in methionine metabolism, with elevated total homocysteine (tHCY) being a biomarker of cardiovascular and cerebrovascular diseases. We evaluated the Abbott ARCHITECT tHCY immunoassay, compared
Kai Fung Kevin Suen, Graham R. Lee, Michelle Finnegan, Kieran Halton, Ingrid Borovickova, Caoilfionn Trench, Maria C. Fitzgibbon   +6 more
doaj  

Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

Molecules, 2017
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. “gold bean” with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng   +5 more
doaj   +1 more source

Determination of chlorine-containing compounds in disinfectants using ion-exchange chromatography

Тонкие химические технологии, 2023
Objectives. To develop a method for the determination of hypochlorite, chloride, chlorite, chlorate, and perchlorate ions in solution; to determine the limits of detection and quantitation for ClO−, Cl−, ClO2−, ClO3−, and ClO4− ions; to evaluate the ...
E. A. Lapina, S. A. Zverev, S. V. Andreev, K. A. Sakharov   +3 more
doaj   +1 more source