Results 11 to 20 of about 558,654 (343)
Ion-exchange chromatography purification of extracellular vesicles
BioTechniques, 2017 Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović +4 more
doaj +2 more sources
Antibody Aggregate Removal by Multimodal Chromatography [PDF]
MoleculesThe growing demand for therapeutic monoclonal antibodies (mAbs) has heightened the need for efficient and scalable purification strategies. A major challenge in downstream processing is the removal of antibody aggregates, which can compromise drug safety,
Veronika Rupčíková +3 more
doaj +2 more sources
PURIFICATION AND PARTIAL CHARACTERIZATION OF SERUM IMMUNOGLOBULIN FROM PERSIAN STURGEON (ACIPENSER PERSICUS) [PDF]
Majallah-i ̒Ilmī-i Shīlāt-i Īrān, 2003 In this study, immunoglobulins from serum of Persian sturgeon (Acipenser persicus) were purified and partially characterized. Immunoglobulins were purified from the pooled sera by a combination of salt precipitation, Ion-exchange chromatography and gel ...
M. Kalbasi1; A. Mostafaei2; J. Majidi3
doaj +2 more sources
Water, electricity and ion exchange; how Hamish Small sustained the evolution of ion chromatography
Heliyon, 2021 Since the development of synthetic ion exchange resins in 1935, the electrochemical properties of these unique materials have intrigued researchers. In 1951, Hamish Small was introduced to synthetic ion exchange resin when he studied the electromigration
John M. Riviello
doaj +1 more source
Analytical Chemistry, 2023 anion-exchange chromatography. The column’s stationary phase is positively charged and equilibrated with a mobile phase containing negative ions (e.g., OH − or another anion) at a minimal concentration. When a sample is introduced with negatively charged
Judith B Ngere +5 more
semanticscholar +1 more source
Effect of ion-exchange chromatography on removal of fibronectin in von Willebrand factor
Zhongguo shuxue zazhi, 2023 Objective To study the removal effect of fibronectin(Fn) from von willebrand factor(vWF) by ion-exchange chromatography through processing human coagulation factor Ⅷ chromatographic washing products, in order to select a method that can effectively ...
Zheng ZHOU +4 more
doaj +1 more source
Journal of Chromatography Open, 2023 In recent decades, there has been a trend toward using larger biological molecules as new active pharmaceutical ingredients (APIs) instead of the classical small organic API molecules.
Torgny Fornstedt, Martin Enmark
doaj +1 more source
Practical Laboratory Medicine, 2022 Objectives: Homocysteine is an intermediary amino acid formed in methionine metabolism, with elevated total homocysteine (tHCY) being a biomarker of cardiovascular and cerebrovascular diseases. We evaluated the Abbott ARCHITECT tHCY immunoassay, compared
Kai Fung Kevin Suen +6 more
doaj +1 more source
The Role of Vibrio cholerae Haemagglutinin Protease (HAP) in Extra-Intestinal Infection [PDF]
Journal of Clinical and Diagnostic Research, 2016 Introduction: Based on the diversity of surface O antigen Vibrio cholerae can be classified into 206 serogroups. Vibrio cholerae is the causative agent of cholera and extra intestinal infections like, septicemia, wound infection and haemorrhagic ...
Amit Ghosh, Hemanta Koley, Amit Pal
doaj +1 more source
The role of lactic acid adsorption by ion exchange chromatography. [PDF]
PLoS ONE, 2010 BACKGROUND: The polyacrylic resin Amberlite IRA-67 is a promising adsorbent for lactic acid extraction from aqueous solution, but little systematic research has been devoted to the separation efficiency of lactic acid under different operating conditions.
Qiang Gao +7 more
doaj +1 more source