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Quantitative Chromosome Conformation Capture
2012It is becoming increasingly apparent that chromatin is not randomly folded into the nucleus, but instead is highly organized into specific conformations within the nucleus. One consequence of such higher order structure is that chromatin looping can bring together genomic elements which are separated by several hundreds of kilobases, such as enhancers ...
Raffaella, Nativio +2 more
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Chromosome Conformation Capture Carbon Copy Technology
Current Protocols in Molecular Biology, 2007AbstractChromosome conformation capture (3C) is used to quantify physical DNA contacts in vivo at high resolution. 3C was first used in yeast to map the spatial chromatin organization of chromosome III, and in higher eukaryotes to demonstrate that genomic DNA elements regulate target genes by physically interacting with them. 3C has been widely adopted
Dostie, Josee, Zhan, Ye, Dekker, Job
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Chromosome Conformation Capture in Drosophila
2016Linear chromatin fiber is packed inside the nuclei as a complex three-dimensional structure, and the organization of the chromatin has important roles in the appropriate spatial and temporal regulation of gene expression. To understand how chromatin organizes inside nuclei, and how regulatory proteins physically interact with genes, chromosome ...
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Chromosome conformation capture assays in bacteria
Methods, 2012Bacterial chromosomes must be compacted by three-orders of magnitude to fit within the cell. While such compaction could in theory yield disordered structures, it is becoming increasingly clear that bacterial chromosomes are in fact arranged in regular and reproducible fashions and that their configurations are tightly connected to fundamental ...
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Capturing pairwise and multi-way chromosomal conformations using chromosomal walks
Nature, 2016Chromosomes are folded into highly compacted structures to accommodate physical constraints within nuclei and to regulate access to genomic information. Recently, global mapping of pairwise contacts showed that loops anchoring topological domains (TADs) are highly conserved between cell types and species. Whether pairwise loops synergize to form higher-
Pedro Olivares-Chauvet +8 more
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Circular Chromosome Conformation Capture in Plants
2017The study of nuclear architecture promises novel insights into genome function and regulation. Hereby, quantitative methods based on chromosome conformation capture (3C) revolutionized the field, as they allow accurate and unbiased characterization of 3D genome organization of genomic regions of interest.
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The Chromosome Conformation Capture (3C) in Drosophila melanogaster
2020The discovery of the DNA double helix by Watson and Crick in 1953 was the first report showing that the genomic information is not contained in a stretched linear molecule. After that, a huge advance in the knowledge of the structure of the eukaryotic genome in the nuclear space has been made over the last decades, bringing us to the widely accepted ...
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Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast
2014Studying interphase chromosome arrangements at the molecular level can provide important details on the function and coordination of many metabolic processes that take place on DNA, such as transcription or DNA repair. The chromosome conformation capture (3C) methodology was originally developed in yeast to study the interphase organization of a single-
Maria D, Mayán, Luis, Aragón
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Chromosome Conformation Capture for Research on Innate Antiviral Immunity
2017Chromosome conformation capture (3C) technology has revolutionized our knowledge on chromatin folding and nuclear organization. This cis-loop detection approach can be used to identify candidate regulatory elements interacting with target gene of interest.
Yoon Jung, Kim, Tae Hoon, Kim
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