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Mapping Chromatin Interactions by Chromosome Conformation Capture
Current Protocols in Molecular Biology, 2006 AbstractChromosome conformation capture (3C) is one of the only techniques that allows for analysis of an intermediate level of chromosome structure ranging from a few to hundreds of kilobases, a level most relevant for gene regulation. The 3C technique is used to detect physical interactions between sequence elements that are located on the same or on
Miele, Adriana +4 more
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Quantitative Chromosome Conformation Capture
Methods in Molecular Biology, 2012It is becoming increasingly apparent that chromatin is not randomly folded into the nucleus, but instead is highly organized into specific conformations within the nucleus. One consequence of such higher order structure is that chromatin looping can bring together genomic elements which are separated by several hundreds of kilobases, such as enhancers ...
Adele Murrell, Raffaella Nativio
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High-resolution circular chromosome conformation capture assay
Nature Protocols, 2008 The pioneering chromosome conformation capture (3C) method provides the opportunity to study chromosomal folding in the nucleus. It is based on formaldehyde cross-linking of living cells followed by enzyme digestion, intramolecular ligation and quantitative (Q)-PCR analysis.
Anita, Göndör +2 more
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Chromosome Conformation Capture
Cold Spring Harbor Protocols, 2009INTRODUCTIONChromosome conformation capture (3C) is a technique used to detect the spatial organization of chromosomal DNA in fixed cells. DNA sequences in spatial proximity in the nucleus or engaged in physical interactions (such as those between genes and regulatory elements) can be assessed quantitatively to provide a measure that potentially ...
Nathan F, Cope, Peter, Fraser
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Capturing Chromosome Conformation
Science, 2002We describe an approach to detect the frequency of interaction between any two genomic loci. Generation of a matrix of interaction frequencies between sites on the same or different chromosomes reveals their relative spatial disposition and provides information about the physical properties of the chromatin fiber.
Job, Dekker +3 more
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Chromosome Conformation Capture of Mitotic Chromosomes
2023Despite more than a century of intensive study of mitotic chromosomes, their three-dimensional organization remains enigmatic. The last decade established Hi-C as a method of choice for study of spatial genome-wide interactions. Although its utilization has been focused mainly on studying genomic interactions in interphase nuclei, the method can be ...
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Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
Journal of Visualized Experiments, 2023Chromosome conformation capture (3C) is a powerful tool that has spawned a family of similar techniques (e.g., Hi-C, 4C, and 5C, referred to here as 3C techniques) that provide detailed information of the three-dimensional organization of chromatin. The 3C techniques have been used in a wide range of studies, from monitoring the changes in chromatin ...
Joniec, Acadia +4 more
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Chromosome Conformation Capture for Large Genomes
2022The gigantic 32Gb Axolotl genome inspires fascinating questions such as: how such a big genome is organized and packed in nuclei and how regulation of gene transcription can happen over such large genomic distances. Currently, there are many technical challenges when we investigate chromatin architecture in axolotl.
Akane, Kawaguchi, Elly M, Tanaka
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Capturing Chromosome Conformation
2020The genome is organized in 3D topology-associated domains to ensure proper gene transcriptional processes. The chromosome conformation capture (3C) is an affordable method to investigate local chromatin structure and dynamics in cells and tissue. Herein I describe an easy to design and a cost-effective protocol.
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Chromosome Conformation Capture Carbon Copy Technology
Current Protocols in Molecular Biology, 2007AbstractChromosome conformation capture (3C) is used to quantify physical DNA contacts in vivo at high resolution. 3C was first used in yeast to map the spatial chromatin organization of chromosome III, and in higher eukaryotes to demonstrate that genomic DNA elements regulate target genes by physically interacting with them. 3C has been widely adopted
Dostie, Josee, Zhan, Ye, Dekker, Job
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