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High-resolution circular chromosome conformation capture assay
Nature Protocols, 2008The pioneering chromosome conformation capture (3C) method provides the opportunity to study chromosomal folding in the nucleus. It is based on formaldehyde cross-linking of living cells followed by enzyme digestion, intramolecular ligation and quantitative (Q)-PCR analysis.
Anita, Göndör +2 more
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Circular Chromosome Conformation Capture in Plants
2017The study of nuclear architecture promises novel insights into genome function and regulation. Hereby, quantitative methods based on chromosome conformation capture (3C) revolutionized the field, as they allow accurate and unbiased characterization of 3D genome organization of genomic regions of interest.
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Chromosome Conformation Capture in Primary Human Cells
20163D organization of the genome, its structural and regulatory function of cell identity, is acquiring prominent features in epigenetics studies; more efforts have been done to develop techniques that allow studying nuclear structure. Chromosome conformation capture (3C) has been set up in 2002 from Dekker and from that moment great investments were made
Alice, Cortesi, Beatrice, Bodega
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The Chromosome Conformation Capture (3C) in Drosophila melanogaster
2020The discovery of the DNA double helix by Watson and Crick in 1953 was the first report showing that the genomic information is not contained in a stretched linear molecule. After that, a huge advance in the knowledge of the structure of the eukaryotic genome in the nuclear space has been made over the last decades, bringing us to the widely accepted ...
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Analysis, Modeling, and Visualization of Chromosome Conformation Capture Experiments
2020Chromatin Conformation Capture techniques have unveiled several layers of chromosome organization such as the segregation in compartments, the folding in topologically associating domains (TADs), and site-specific looping interactions. The discovery of this genome hierarchical organization emerged from the computational analysis of chromatin capture ...
Marco, Di Stefano +5 more
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Chromosome Conformation Capture (3C and Higher) with Erythroid Samples
2017Chromosome conformation capture (3C) allows for the determination of the proximity in nuclei of DNA sequences that are linearly distant from one another in the genome. Proximity that is above that expected from random interaction provides evidence for potential long-range functional interactions such as between enhancers and their target genes.
Ivan, Krivega, Ann, Dean
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Technical Review: A Hitchhiker’s Guide to Chromosome Conformation Capture
2017The introduction of chromosome conformation capture (3C) technologies boosted the field of 3D-genome research and significantly enhanced the available toolset to study chromosomal architecture. 3C technologies not only offer increased resolution compared to the previously dominant cytological approaches but also allow the simultaneous study of genome ...
Stefan, Grob, Giacomo, Cavalli
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Chromosome Conformation Capture for Research on Innate Antiviral Immunity
2017Chromosome conformation capture (3C) technology has revolutionized our knowledge on chromatin folding and nuclear organization. This cis-loop detection approach can be used to identify candidate regulatory elements interacting with target gene of interest.
Yoon Jung, Kim, Tae Hoon, Kim
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Chromosome Conformation Capture on Chip (4C): Data Processing
20164C methods are useful to investigate dependencies between regulatory mechanisms and chromatin structures by revealing the frequency of chromatin contacts between a locus of interest and remote sequences on the chromosome. In this chapter we describe a protocol for the data analysis of microarray-based 4C experiments, presenting updated versions of the ...
Leblanc, B +3 more
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Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast
2014Studying interphase chromosome arrangements at the molecular level can provide important details on the function and coordination of many metabolic processes that take place on DNA, such as transcription or DNA repair. The chromosome conformation capture (3C) methodology was originally developed in yeast to study the interphase organization of a single-
Maria D, Mayán, Luis, Aragón
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