Results 121 to 130 of about 44,698 (271)

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

Advanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son, Goeun Kim, Jungjoon K. Lee   +2 more
wiley   +1 more source

Comparative Single‐Cell Transcriptomic Atlas Reveals the Genetic Regulation of Reproductive Traits

Advanced Science, EarlyView.
A cross‐species single‐cell transcriptomic atlas of reproductive and central nervous system tissues from sheep and humans reveals conserved cellular programs and regulatory networks that regulated fertility. Integration with GWAS for sheep lifetime average litter size identifies UNC5–SLIT–BMP signaling as a core pathway coordinating neuroendocrine ...
Bingru Zhao, Hanpeng Luo, Dailu Guan, Xuefeng Fu, Feng Wang, Guoming Zhang, Shanglai Li, Hua Yang, Yanli Zhang   +8 more
wiley   +1 more source

Characteristics and phylogenetic distribution of megaplasmids and prediction of a putative chromid in Pseudomonas aeruginosa

Computational and Structural Biotechnology Journal
Research on megaplasmids that contribute to the spread of antimicrobial resistance (AMR) in Pseudomonas aeruginosa strains has grown in recent years due to the now widely used technologies allowing long-read sequencing.
Nanfei Wang, Xuan Zheng, Sebastian Leptihn, Yue Li, Heng Cai, Piaopiao Zhang, Wenhao Wu, Yunsong Yu, Xiaoting Hua   +8 more
doaj   +1 more source

Penalized EM algorithm and copula skeptic graphical models for inferring networks for mixed variables

, 2014
In this article, we consider the problem of reconstructing networks for continuous, binary, count and discrete ordinal variables by estimating sparse precision matrix in Gaussian copula graphical models.
Abegaz, Fentaw, Wit, Ernst
core   +1 more source

NanoLoop: A Deep Learning Framework Leveraging Nanopore Sequencing for Chromatin Loop Prediction

Advanced Science, EarlyView.
Chromatin loops are central to gene regulation and 3D genome organization. Leveraging Nanopore sequencing's ability to jointly capture DNA sequence and methylation, we present NanoLoop, the first framework for genome‐wide chromatin loop prediction using Nanopore data.
Wenjie Huang, Li Tang, Matthew C. Hill, Yonghao Zhang, Jun Xie, Min Li   +5 more
wiley   +1 more source

Corrigendum: Dynamic nature of SecA and Its associated proteins in Escherichia coli

Frontiers in Microbiology, 2020
Shun Adachi, Yasuhiro Murakawa, Sota Hiraga   +2 more
doaj   +1 more source

Multi‐Tissue Genetic Regulation of RNA Editing in Pigs

Advanced Science, EarlyView.
This study presents the first multi‐tissue map of RNA editing and its genetic regulation in pigs. By integrating RNA editing profiles, edQTL mapping, GWAS, and cross‐species comparisons, this work establishes RNA editing as a distinct regulatory layer linking genetic variation to complex traits, highlighting its functional and evolutionary significance.
Xiangchun Pan, Wentao Gong, Xiaodian Cai, Jinyan Teng, Jiali Cai, Haonan Zeng, Wondossen Ayalew, Qingpeng Shen, Zhanming Zhong, Yifei Wang, Wenjing Zhang, Yuhan Tian, Dantong Xu, Yahui Gao, Hongwei Yin, Yuebo Zhang, Jiahui Hou, Tianru Zhou, Jiaqi Li, Lingzhao Fang, Xiaolong Yuan, Zhe Zhang   +21 more
wiley   +1 more source

Redistribution of fragmented mitochondria ensures symmetric organelle partitioning and faithful chromosome segregation in mitotic mouse zygotes

eLife
In cleavage-stage embryos, preexisting organelles partition evenly into daughter blastomeres without significant cell growth after symmetric cell division.
Haruna Gekko, Ruri Nomura, Daiki Kuzuhara, Masato Kaneyasu, Genpei Koseki, Deepak Adhikari, Yasuyuki Mio, John Carroll, Tomohiro Kono, Hiroaki Funahashi, Takuya Wakai   +10 more
doaj   +1 more source

Genetic relationships and diversity among wild Salvia accessions revealed by ISSR and SCoT markers

Biotechnology & Biotechnological Equipment, 2018
In this study, inter-simple sequence repeats (ISSR) and start codon targeted (SCoT) markers were used for genetic diversity and relationship analysis of nine Salvia species. Twenty-one and twenty selected ISSR and SCoT primers amplified 350 and 329 loci,
Alireza Etminan, Alireza Pour-Aboughadareh, Afsaneh Noori, Amin Ahmadi-Rad, Lia Shooshtari, Zahra Mahdavian, Masoumeh Yousefiazar-Khanian   +6 more
doaj   +1 more source

Parasitic Plant–Host Interactions: Molecular Mechanisms and Agricultural Resistance Strategies

Advanced Science, EarlyView.
Parasitic plants devastate global agriculture through sophisticated molecular interactions with host crops. This review synthesizes current understanding of parasitic plant‐host interactions, from strigolactone‐mediated germination and haustorium formation to host defense mechanisms.
Jiayang Shi, Qi Xie, Feifei Yu
wiley   +1 more source