Results 21 to 30 of about 693,747 (160)

Cdt1 stabilizes an open MCM ring for helicase loading

Nature Communications, 2017
The loading and activation of the Mcm2-7 replicative helicase couples cell cycle progression to DNA replication. Here the authors use X-ray crystallography and single-particle electron microscopy to demonstrate how Ctd1 functions to promote MCM loading ...
Jordi Frigola, Jun He, Kerstin Kinkelin, Valerie E. Pye, Ludovic Renault, Max E. Douglas, Dirk Remus, Peter Cherepanov, Alessandro Costa, John F. X. Diffley   +9 more
doaj   +1 more source

Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication

eLife, 2020
DNA replication is needed to duplicate a cell’s genome in S phase and segregate it during cell division. Previous work in Leishmania detected DNA replication initiation at just a single region in each chromosome, an organisation predicted to be ...
Jeziel Dener Damasceno, Catarina A Marques, Dario Beraldi, Kathryn Crouch, Craig Lapsley, Ricardo Obonaga, Luiz RO Tosi, Richard McCulloch   +7 more
doaj   +1 more source

Chromosomal macrodomains and associated proteins : implications for DNA organization and replication in gram negative bacteria [PDF]

, 2011
The Escherichia coli chromosome is organized into four macrodomains, the function and organisation of which are poorly understood. In this review we focus on the MatP, SeqA, and SlmA proteins that have recently been identified as the first examples of ...
Kalmykowa, Olga J., Remus T. Dame, Kalmykowa, OJ, Dame Remus T., Grainger David C., Dame, Remus T. (Remus Thei), David C Grainger, Grainger, David, Kalmykowa Olga J., David C. Grainger, Dame, RT, Olga J Kalmykowa, Grainger, David C., Remus T Dame, Olga J. Kalmykowa   +14 more
core   +1 more source

Genome organization of DNA replication timing and its link to chromatin and transcription [PDF]

, 2008
The replication of the genome is a highly organized process. Not every sequence replicates at the same time, instead some genes replicate early, while others replicate later during S phase.
Schwaiger, Michaela
core   +1 more source

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

Nature Communications, 2018
Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase, which requires the leading-strand polymerase Pol ɛ. Here the authors present a structural analysis of a CMG Pol ɛ on a DNA fork, providing insight on the steps leading ...
Panchali Goswami, Ferdos Abid Ali, Max E. Douglas, Julia Locke, Andrew Purkiss, Agnieszka Janska, Patrik Eickhoff, Anne Early, Andrea Nans, Alan M. C. Cheung, John F. X. Diffley, Alessandro Costa   +11 more
doaj   +1 more source

Asynchronous replication, mono-allelic expression, and long range Cis-effects of ASAR6. [PDF]

PLoS Genetics, 2013
Mammalian chromosomes initiate DNA replication at multiple sites along their length during each S phase following a temporal replication program. The majority of genes on homologous chromosomes replicate synchronously. However, mono-allelically expressed
Nathan Donley, Eric P Stoffregen, Leslie Smith, Christina Montagna, Mathew J Thayer   +4 more
doaj   +1 more source

Ultrafine anaphase bridges, broken DNA and illegitimate recombination induced by a replication fork barrier [PDF]

, 2011
Most DNA double-strand breaks (DSBs) in S- and G2-phase cells are repaired accurately by Rad51-dependent sister chromatid recombination. However, a minority give rise to gross chromosome rearrangements (GCRs), which can result in disease/death.
Ledesma, Jennifer, Whitby, Matthew C., Castagnetti, S, Sofueva, Sevil, Alexander Lorenz, Steinacher, R, Fekret Osman, Lorenz, Alexander, Sofueva, S, Ledesma, J, Castagnetti, Stefania, Whitby, Matthew, Steinacher, Roland, Sevil Sofueva, Stefania Castagnetti, Osman, F, Lorenz, A, Matthew C. Whitby, Whitby, MC, Osman, Fekret, Roland Steinacher, Jennifer Ledesma   +21 more
core   +1 more source

Prokaryotic ParA–ParB–parS system links bacterial chromosome segregation with the cell cycle [PDF]

, 2012
While the essential role of episomal par loci in plasmid DNA partitioning has long been appreciated, the function of chromosomally encoded par loci is less clear. The chromosomal parA–parB genes are conserved throughout the bacterial kingdom and encode
Mierzejewska, Jolanta, Jolanta Mierzejewska, Grażyna Jagura-Burdzy, Jagura-Burdzy, Grazyna   +3 more
core   +1 more source

Cryo-EM structure of a licensed DNA replication origin

Nature Communications, 2017
Origins of replication are licensed by loading of MCM onto DNA, and origin firing depends on interaction with Cdc45 and GINS to form two CMG holo-helicases. Here, authors determine the cryo-EM structures of DNA-bound MCM and visualise a phospho-dependent
Ferdos Abid Ali, Max E. Douglas, Julia Locke, Valerie E. Pye, Andrea Nans, John F. X. Diffley, Alessandro Costa   +6 more
doaj   +1 more source

Chromosome replication in Myxococcus xanthus [PDF]

Journal of Bacteriology, 1978
The rates of DNA synthesis during the cell-division cycle were measured in Myxococcus xanthus growing in three different media permitting a twofold variation in doubling time. In all three media, simple DNA cycles were observed. Synthesis of DNA occurred during 85% of the cell-division cycle, independent of generation time, from 5 to 11 h.
D R, Zusman, D M, Krotoski, M, Cumsky
openaire   +2 more sources