Synthetic biology: advancing biological frontiers by building synthetic systems [PDF]
, 2012 Advances in synthetic biology are contributing to diverse research areas, from basic biology to biomanufacturing and disease therapy.
Chen, Yvonne Y. +2 more
core +5 more sources
Mechanisms for Chromosome Segregation in Bacteria
Frontiers in Microbiology, 2021 The process of DNA segregation, the redistribution of newly replicated genomic material to daughter cells, is a crucial step in the life cycle of all living systems.
Christos Gogou +2 more
doaj +1 more source
TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. [PDF]
, 2015 The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family.
Corbett, Kevin D +5 more
core +2 more sources
Spatial organization of bacterial chromosomes [PDF]
Current Opinion in Microbiology, 2014 Bacterial chromosomes are organized in stereotypical patterns that are faithfully and robustly regenerated in daughter cells. Two distinct spatial patterns were described almost a decade ago in our most tractable model organisms. In recent years, analysis of chromosome organization in a larger and more diverse set of bacteria and a deeper ...
Xindan, Wang, David Z, Rudner
openaire +2 more sources
Optically mapping multiple bacterial genomes simultaneously in a single run. [PDF]
PLoS ONE, 2011 Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this ...
Matthew C Riley +3 more
doaj +1 more source
Origin of spatial organization of DNA-polymer in bacterial chromosomes
, 2017 In-vivo DNA organization at large length scales ($\sim 100nm$) is highly debated and polymer models have proved useful to understand the principle of DNA-organization. Here, we show that $
Agarwal, Tejal +3 more
core +1 more source
A Powerful New Quantitative Genetics Platform, Combining Caenorhabditis elegans High-Throughput Fitness Assays with a Large Collection of Recombinant Strains. [PDF]
, 2015 The genetic variants underlying complex traits are often elusive even in powerful model organisms such as Caenorhabditis elegans with controlled genetic backgrounds and environmental conditions.
Andersen, Erik C +7 more
core +2 more sources
Bacterial artificial chromosomes improve recombinant protein production in mammalian cells
BMC Biotechnology, 2009 Background The development of appropriate expression vectors for large scale protein production constitutes a critical step in recombinant protein production.
Bauer Anton +4 more
doaj +1 more source
Structure of metaphase chromosomes: a role for effects of macromolecular crowding. [PDF]
PLoS ONE, 2012 In metaphase chromosomes, chromatin is compacted to a concentration of several hundred mg/ml by mechanisms which remain elusive. Effects mediated by the ionic environment are considered most frequently because mono- and di-valent cations cause ...
Ronald Hancock
doaj +1 more source
Bacterial chromosome extraction and isolation [PDF]
Lab on a Chip, 2002 We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.
Christelle, Prinz +4 more
openaire +2 more sources