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Buffalo (Bos Buffali L.) chymosin purification and properties

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1996
Buffalo chymosin was isolated from abomasum mucosa extract of buffalo calves by affinity chromatography on gramicidin S-agarose followed by ion exchange chromatography on gamma-aminopropylsilochrom. Its molecular weight, 36 +/- 1 kDa, is similar to that of bovine calf chymosin. The N-terminal sequence Gly-Glu-Val-Ala-Ser-Val-Pro- coincides with that of
C A, Abdel Malak   +6 more
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Enzymes: Chymosin and Other Milk Coagulants

2010
The enzymatic coagulation of milk involves limited proteolysis of the caseins by enzymes in preparations called rennets, followed by aggregation of the rennet-altered casein micelles in the presence of calcium ions and at temperatures greater than  ~ 18°C.
McSweeney, P. L. H., Bansal, N.
openaire   +2 more sources

Sensitive fluorometric assay for the activity of chymosin

International Journal of Peptide and Protein Research, 1996
Fluorogenic substrates for chymosin [Dns‐Leu‐Ser‐Phe‐Trp‐Ala‐Leu‐OCH2Py (I), Dns‐Leu‐Ser‐Phe‐Met‐Trp‐Leu‐OCH2Py (II), Dns‐Leu‐Ser‐Leu‐Trp‐Ala‐Leu‐OCH2Py (III), Dns‐Leu‐Ala‐Phe‐Trp‐Ala‐Leu‐OCH2Py (IV), Dns‐Leu‐Ser‐Phe‐Leu‐Ala‐Leu‐OCH2Py (V) and Dns‐Leu‐Ser‐Phe‐Phe‐Ala‐Leu‐OCH2Py (VI)] were synthesized by a solution method.
H, Yonezawa   +3 more
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Analysis of Some Biochemical Properties of Recombinant Siberian Roe Deer (Capreolus pygargus) Chymosin Obtained in the Mammalian Cell Culture (CHO-K1)

Biochemistry (Moscow), 2023
D. E. Murashkin   +4 more
semanticscholar   +1 more source

Bovine chymosin

Biotechnology Advances, 1999
A.K Mohanty   +3 more
openaire   +1 more source

Fluorogenic substrates for assay of chymosin.

Biochemistry. Biokhimiia, 2000
The use of fluorogenic substrates with intramolecular fluorescence quenching as substrates for chymosin was studied. It was shown that chymosin hydrolyzes the Phe-Phe peptide bond. The effect of pH on the hydrolysis of substrates by chymosin was investigated. The catalytic characteristics of the hydrolysis of the fluorogenic substrates were obtained at
V V, Starovoitova   +4 more
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Production of Beluga Whale (Delphinapterus leucas) Chymosin in the Methylotrophic Yeast Komagataella phaffii and Characteristics of the Recombinant Enzyme

Applied Biochemistry and Microbiology, 2021
S. Filkin   +5 more
semanticscholar   +1 more source

Lab, Chymosin

1927
Hans v. Euler, Karl Myrbäck
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