Results 51 to 60 of about 49,239 (220)

Peptidolytic enzymes in different larval stadium of housefly Musca domestica

open access: yesVeterinární Medicína, 2006
Four classes of peptidolytic enzymes were described in insects. Many authors have found predominant activity belonging to trypsin-like and chymotrypsin-like activity.
J. Blahovec, Z. Kostecka, A. Kocisova
doaj   +1 more source

Enabling Variant Proteogenomic Assay Development Through a Peptide Target Selection Pipeline and Web Application

open access: yesPROTEOMICS, EarlyView.
ABSTRACT The quantification of key protein variants can guide precision oncology to enable better identification of driver mutations. Bottom‐up assays infer parent protein levels through the quantification of surrogate peptides. Assay methods designed for absolute quantification of protein variants, such as multiple reaction monitoring mass ...
Constantinos Blidjios   +4 more
wiley   +1 more source

EFFECT OF α–CHYMOTRYPSIN AND GELATIN SUPPLEMENTATION IN RABBIT SEMEN EXTENDER ON ANTI-SPERM ANTIBODYS AND FERTILITY [PDF]

open access: yesEgyptian Journal of Rabbit Science, 2015
Three experiments were carried out to study the effects of α-chymotrypsin with gelatin on anti-sperm antibodies (ASAs) in rabbit's semen and fertilization rate. In the first experiment, all rabbits males (20 bucks) were tested for the presence of ASAs in
Mohamed elspeiy   +2 more
doaj   +1 more source

Unspecific Molecular Adsorption (UMA) Sample Preparation Method for Bottom‐up and Whole Protein Analysis: The Foundation

open access: yesPROTEOMICS, EarlyView.
ABSTRACT The protein sample preparation methods for shotgun proteomics are nowadays well‐established unlike the ones for whole protein analysis. The goal of my work has been to create a simple methodology which provides a single uncomplicated sample preparation tool for these two fields. Nowadays the bulk of proteomics work is done using detergents for
Alexandre Zougman
wiley   +1 more source

Cross‐Linking Mass Spectrometry of the Antimicrobial Peptides Magainin 2 and PGLa Reveals Heterodimerization in Micellar Medium

open access: yesRapid Communications in Mass Spectrometry, EarlyView.
ABSTRACT Rationale In this study, we applied cross‐linking mass spectrometry (XL‐MS) to characterize the oligomeric states of a PGLa/magainin 2 mixture and gain insight into the heterodimerization previously suggested in the literature. Both peptides have shown a synergistic enhancement of activity when tested in antimicrobial assays; however, the ...
Emilie Hirschler   +7 more
wiley   +1 more source

Peptide‐based antibacterial nanoplatforms: Design principles, stimuli‐regulated behaviors, and applications

open access: yesResponsive Materials, EarlyView.
Peptide‐based antibacterial nanoplatforms, encompassing self‐assembled peptides and peptide‐engineered inorganic, polymeric, and lipid nanocarriers, are systematically reviewed. The article highlights design principles and stimuli‐responsive regulation for improving peptide stability, delivery efficiency, and antibacterial performance.
Peng Tan   +8 more
wiley   +1 more source

Fluorination as a Molecular Design Parameter for Programming Peptide Nanocarriers

open access: yesSmall, EarlyView.
Controlled side‐chain fluorination programs the SA, morphology, and biological performance of amphiphilic fluoropeptide–RGD nanocarriers. A narrow fluorination window yields compact non‐amyloid fibrils with high loading capacity, pH‐triggered release, receptor‐specific uptake, low cytotoxicity, and enzymatic degradability.
Maurizio Iannuzzi   +10 more
wiley   +1 more source

Hydrophobic interaction chromatography as separation method of alkaline proteases from viscera of Scomberomorus sierra

open access: yesTIP Revista Especializada en Ciencias Químico-Biológicas, 2019
This study focused on recovering alkaline proteases from the viscera of Scomberomorus sierra through hydrophobic interaction chromatography. Three alkaline proteases were partially separated using this chromatographic technique; two of them, with ...
Pablo Sergio Osuna-Amarillas   +6 more
doaj   +1 more source

Rational design of a lipophilic β‐galactosidase‐responsive near‐infrared probe for in vivo imaging of cellular senescence

open access: yesSmart Molecules, EarlyView.
We developed a lipophilic near‑infrared probe (DCIP‑AcGal) that is specifically activated by senescence‑associated β‑galactosidase. After intravenous injection, it lights up therapy‑induced tumor senescence in living mice. Its optimized lipophilicity also enables blood‑brain barrier crossing to detect natural brain senescence ex vivo. This probe offers
Jiani Huang   +15 more
wiley   +1 more source

Iflaviruses in arthropods: when small is mighty

open access: yesInsect Science, EarlyView.
Many arthropod species harbor iflaviruses, which often cause covert (asymptomatic) infections, but may still affect host fitness. We review the impact of iflaviruses on arthropod fitness, immunity, behaviour as well as the iflavirus’ host range, transmission, tissue tropism and the interactions with other microorganisms within arthropods.
Annamaria Mattia   +3 more
wiley   +1 more source

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