Results 71 to 80 of about 57,869 (249)
PROTEOMICS, EarlyView.ABSTRACT The quantification of key protein variants can guide precision oncology to enable better identification of driver mutations. Bottom‐up assays infer parent protein levels through the quantification of surrogate peptides. Assay methods designed for absolute quantification of protein variants, such as multiple reaction monitoring mass ...
Constantinos Blidjios +4 more
wiley +1 more source
PROTEOMICS, EarlyView.ABSTRACT The protein sample preparation methods for shotgun proteomics are nowadays well‐established unlike the ones for whole protein analysis. The goal of my work has been to create a simple methodology which provides a single uncomplicated sample preparation tool for these two fields. Nowadays the bulk of proteomics work is done using detergents for
Alexandre Zougman
wiley +1 more source
Isolation and characterisation of two chymotrypsins from Allocyttus niger (black oreo dory) viscera
, 1997 Two serine proteases from the viscera of deep-sea fish, black oreo dory (Allocyttus niger),were purified by hydrophobic, affinity, and cation exchange chromatography.
Krzyzosiak, J., Daniel, Roy M.
core
Bt agave: why it is time to explore a new biotechnological frontier
Pest Management Science, EarlyView.Drylands cover 41% of Earth, requiring sustainable crops. Agave, drought‐ and heat‐adapted, offers high‐value products with low water needs. Pests limit yield, yet Bacillus thuringiensis (Bt) Cry proteins, successful in other plants, remain unexploited in Agave.
Aline Vitória Corim Marim +3 more
wiley +1 more source
Reversible “Irreversible” Inhibition of Chymotrypsin Using Nanoparticle Receptors
, 2016 Anionically functionalized amphiphilic nanoparticles efficiently inhibit chymotrypsin through electrostatic binding followed by protein denaturation.
Catherine M. Goodman (2726062) +4 more
core +1 more source
Rapid Communications in Mass Spectrometry, EarlyView.ABSTRACT Rationale In this study, we applied cross‐linking mass spectrometry (XL‐MS) to characterize the oligomeric states of a PGLa/magainin 2 mixture and gain insight into the heterodimerization previously suggested in the literature. Both peptides have shown a synergistic enhancement of activity when tested in antimicrobial assays; however, the ...
Emilie Hirschler +7 more
wiley +1 more source
TIP Revista Especializada en Ciencias Químico-Biológicas, 2019 This study focused on recovering alkaline proteases from the viscera of Scomberomorus sierra through hydrophobic interaction chromatography. Three alkaline proteases were partially separated using this chromatographic technique; two of them, with ...
Pablo Sergio Osuna-Amarillas +6 more
doaj +1 more source
PURIFICATION OF CHYMOTRYPSIN FROM FISH WASTE USING REVERSE MICELLES
, 2011 Fish processing waste can be used to produce commercially valuable by-products, such as chymotrypsin which has application in various industries including the food industry, leather production industry and chemical industry.
Zhou, Liang
core
Α-Chymotrypsin in water-ethanol mixtures: Effect of preferential interactions [PDF]
, 2017 © 2017 Elsevier B.V. We investigated preferential interactions of α-chymotrypsin with water–ethanol mixtures at 25 °C. Our approach is based on the analysis of residual enzyme activity and water/alcohol sorption. There are three concentration regimes.
Kuchierskaya A. +3 more
core +1 more source
Responsive Materials, EarlyView.Peptide‐based antibacterial nanoplatforms, encompassing self‐assembled peptides and peptide‐engineered inorganic, polymeric, and lipid nanocarriers, are systematically reviewed. The article highlights design principles and stimuli‐responsive regulation for improving peptide stability, delivery efficiency, and antibacterial performance.
Peng Tan +8 more
wiley +1 more source