Results 61 to 70 of about 4,619 (224)

SPROUTS_DB: An Implemented Database of Contaminants for Extracellular Vesicle Proteomics Studies

PROTEOMICS, EarlyView.
ABSTRACT Current proteomics techniques allow rapid identification and quantification of proteins within any given biological source. However, LC–MS/MS proteomics is vulnerable to laboratory and sample‐associated contaminants. Therefore, accurate identification and annotation of such contaminants is crucial for development of reliable databases ...
Maria Gaetana Giovanna Pittalà, Loredana Leggio, Greta Paternò, Elena Giusto, Laura Civiero, Vincenzo Cunsolo, Silvia Vivarelli, Antonella Di Francesco, Emanuele Alpi, Nunzio Iraci, Rosaria Saletti   +10 more
wiley   +1 more source

Structural and biochemical insights of CypA and AIF interaction [PDF]

, 2017
The Cyclophilin A (CypA)/Apoptosis Inducing Factor (AIF) complex is implicated in the DNA degradation in response to various cellular stress conditions, such as oxidative stress, cerebral hypoxia-ischemia and traumatic brain injury.
Caporale, Andrea, Chambery, Angela, Di Sorbo, Gianluigi, Doti, Nunzianna, Farina, Biancamaria, Fattorusso, Roberto, Leoni, Guido, Mascanzoni, Fabiola, Raimondo, Domenico, Russo, Rosita, Ruvo, Menotti   +10 more
core   +1 more source

Targeting protein–protein interactions with reversible covalent modalities: Non‐cysteine chemistries

British Journal of Pharmacology, EarlyView.
Abstract Protein–protein interactions (PPIs) are central to diverse cellular functions, and represent a rapidly expanding class of therapeutic targets. Advancements in covalent drug design have enabled small‐molecule drugs to overcome challenges associated with engaging these targets, such as limited durations of action and difficult‐to‐drug (expansive,
Ruchira Basu, Steven Fletcher
wiley   +1 more source

PPIases Par14/Par17 Affect HBV Replication in Multiple Ways

Viruses, 2023
Human parvulin 14 (Par14) and parvulin 17 (Par17) are peptidyl-prolyl cis/trans isomerases that upregulate hepatitis B virus (HBV) replication by binding to the conserved 133Arg-Pro134 (RP) motif of HBc and core particles, and 19RP20-28RP29 motifs of HBx.
Kyongmin Kim
doaj   +1 more source

The competitive interplay of 12‐oxophytodienoic acid (OPDA), protein thiols, and glutathione

The FEBS Journal, EarlyView.
12‐Oxophytodienoic acid (OPDA) is a phytohormone involved in plant growth and stress defense. Due to its cyclopentenone moiety, OPDA can form Michael adducts with thiol‐containing compounds such as glutathione and cysteine residues of proteins, resulting in alterations of the cellular redox regulatory network.
Madita Knieper, Ruben Schwarz, Lara Vogelsang, Jens Sproß, Armağan Kaya, Maike Bittmann, Harald Gröger, Andrea Viehhauser, Karl‐Josef Dietz   +8 more
wiley   +1 more source

Novel Regulation of Alpha-Toxin and the Phenol-Soluble Modulins by Peptidyl-Prolyl cis/trans Isomerase Enzymes in Staphylococcus aureus

Toxins, 2019
Peptidyl-prolyl cis/trans isomerases (PPIases) are enzymes that catalyze the cis-to-trans isomerization around proline bonds, allowing proteins to fold into their correct confirmation. Previously, we identified two PPIase enzymes in Staphylococcus aureus
Rebecca A. Keogh, Rachel L. Zapf, Emily Trzeciak, Gillian G. Null, Richard E. Wiemels, Ronan K. Carroll   +5 more
doaj   +1 more source

Proteomic analysis of Oesophagostomum dentatum (Nematoda) during larval transition, and the effects of hydrolase inhibitors on development [PDF]

, 2013
In this study, in vitro drug testing was combined with proteomic and bioinformatic analyses to identify and characterize proteins involved in larval development of Oesophagostomum dentatum, an economically important parasitic nematode.
Gasser, Robin B, Joachim, Anja, Mitreva, Makedonka, Ondrovics, Martina, Razzazi-Fazeli, Ebrahim, Silbermayr, Katja, Young, Neil D   +6 more
core   +4 more sources

Current perspectives on KMT2A fusion proteins and menin inhibition in paediatric acute myeloid leukaemia

The FEBS Journal, EarlyView.
Genetic rearrangements resulting in the expression of KMT2A fusion alleles can lead to dramatic transcriptional disturbances that contribute to the onset of acute leukaemias. Fortunately, menin inhibition has emerged as a promising new class of targeted therapy.
Lydia Elaine Roets, Graeme Greenfield, Katrina Mairead Lappin   +2 more
wiley   +1 more source

Optimized E.coli expression strain LOBSTR eliminates common contaminants from His-tag purification [PDF]

, 2013
His-tag affinity purification is one of the most commonly used methods to purify recombinant proteins expressed in E. coli. One drawback of using the His-tag is the co-purification of contaminating histidine-rich E. coli proteins.
Andersen, Kasper R., Leksa, Nina Carolina, Schwartz, Thomas   +2 more
core   +1 more source

The GmGT‐2F, a trihelix transcription factor, regulates seed oil content by directly activating GmAGAL transcription in soybean

Journal of Integrative Plant Biology, EarlyView.
In soybean, the trihelix transcription factor GmGT‐2F positively regulates the expression of the α‐galactosidase gene GmAGAL, thereby modulating seed oil content. The cyclophilin GmCYP2 interacts with GmGT‐2F and suppresses GmAGAL activation. Haplotype diversity analysis of GmGT‐2F, GmCYP2, and GmAGAL identified favorable haplotype combinations ...
Shuangzhe Li, Yifan Cui, Yang Liu, Mingliang Yang, Xiaoxia Wu, Qingshan Chen, Ying Zhao   +6 more
wiley   +1 more source