Results 311 to 320 of about 4,089,902 (335)

A novel ELISA for the evaluation of the classical pathway of complement

Journal of Immunological Methods, 1998
Assessment of the overall function of the classical pathway of complement is traditionally performed by the hemolytic titration assay CH50. In the present study, we established a novel method for the quantitation of complement activity by measuring the deposition of C1q, C4, C3 and C9 on solid-phase IgM by an enzyme-linked immunosorbent assay (ELISA ...
Otto Götze, A Wittig, Jörg Zwirner, E Kremmer   +3 more
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Classical Signaling Pathways

2014
In this chapter, I introduce a number of important aspects of intracellular signaling pathways related to the protection and degeneration in cells, especially neurons, under physiological and pathological conditions. Extracellular stimuli activate intracellular signaling pathways by receptor- and/or channel-mediated manner or simple diffusion across ...
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Targetable molecular pathways in classical Hodgkin's lymphoma

Expert Opinion on Investigational Drugs, 2011
most patients with classical Hodgkin's lymphoma (cHL) are cured by stage-adapted multimodal regimens. However, some will suffer from refractory disease or experience a relapse. Furthermore, late toxicity due to aggressive chemotherapy and/or radiotherapy has become an increasing problem in long-term survivors.
Adams, Heiner, Obermann, Ellen C., Dirnhofer, Stephan, Tzankov, Alexandar   +3 more
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Activation of the classical complement pathway by BioRex-70

Immunology Letters, 1987
The cation exchange resin BioRex-70 was able to activate the classical complement pathway in human serum at 37 degrees C over the resin concentration range 0-5% (v/v). Using zymosan-treated human serum, it was found that the activation proceeded as far as complement protein C3.
S.B. Easterbrook-Smith, R.J. Vandenberg
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Interaction of fucoidan with the proteins of the complement classical pathway

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2003
Fucoidan inhibits complement by mechanisms that so far remain to be unraveled, and the objective of this work was to delineate the mode of inhibition by this sulfated polysaccharide. For that purpose, low molecular weight fractions of algal (Ascophyllum nodosum) fucoidan containing the disaccharide unit [-->3)-alpha-L-Fuc(2SO3(-))-(1-->4)-alpha-L-Fuc(2,
Stéphanie Descroix, Pierre Gareil, Bérangère Tissot, Anne Varenne, Béatrice Montdargent, Régis Daniel, Lionel Chevolot   +6 more
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Aldo-keto reductase family 1, member B10 is secreted through a lysosome-mediated non-classical pathway.

Biochemical Journal, 2011
AKR1B10 (aldo-keto reductase family 1, member B10) protein is primarily expressed in normal human small intestine and colon, but overexpressed in several types of human cancers and considered as a tumour marker.
Di-xian Luo, Mei C. Huang, Jun Ma, Zachary Gao, D. Liao, D. Cao   +5 more
semanticscholar   +1 more source

Classical and Alternative Pathways of Mast Cell Activation

Critical Reviews™ in Immunology, 2002
It has long since been recognized that mast cells are critical effectors of anaphylactic reactions, and the existence of these potentially hazardous cells has solely been justified due to their beneficial role in some infections with extracellular parasites. A novel understanding of mast cells as sentinels of the immune system has been made possible by
Lothar Hültner, Edgar Schmitt, Michael Stassen   +2 more
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An immunofluorescence assay for complement activation by the classical pathway

Journal of Immunological Methods, 1981
The functional integrity of classical complement pathway components was determined by an immunofluorescence (IFL) assay based on the capacity of cytoskeletal intermediate filaments (IMF) to bind C1q and to activate the complement pathway. The assay uses IMF-rich capillary endothelium of human term placentae as complement-activating substrate.
S. Meri, M. Rhen, Ewert Linder
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The Initiation Complexes of the Classical and Lectin Pathways

2006
Various antigen structures initiate the classical and the lectin pathways of complement activation. Multidomain modular proteases (C1r, C1s and MASPs) are involved in the initiation complexes of both pathways. Despite the identical domain organization of these serine proteases there are differences in their specificities and functions.
Géza Ambrus, Péter Závodszky, Zsolt Lőrincz, Péter Gál   +3 more
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Classical pathway deficiencies – A short analytical review

Molecular Immunology, 2015
Deficiencies in the classical pathway of complement activation have some common features but show also great differences. Deficiencies of each of the components (C1q, C1s, C1r, C4 and C2) imply increased susceptibility to bacterial infections. They are also associated with increased risk to develop systemic lupus erythematosus where deficiency of C1q ...
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