Results 101 to 110 of about 107,781 (288)

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

The Impact of Splicing Factor Mutations on Clonal Hematopoiesis and Myeloid Neoplasm Progression

Frontiers in Bioscience-Landmark
Clonal hematopoiesis (CH) is characterized by the expansion of hematopoietic stem and progenitor cells harboring somatic mutations, which confers an increased risk of hematologic malignancies and cardiovascular disease.
Takuya Izumi-Tamura, Asuka Kawachi, Akihide Yoshimi   +2 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Clones in blood and stratified epithelial cells, and their drivers

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
The term “clone” is commonly used in the medical and life sciences to denote a genetically identical population, at both the individual and cellular levels. The concept of clonal expansion is of fundamental importance in cancer research.
Shigeru CHIBA
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Novel non-motile phenotypes of Yersinia ruckeri suggest expansion of the current clonal complex theory

, 2011
The biochemical and cell surface characteristics of 63 non-motile isolates of Yersinia ruckeri from various sources were compared using the API 20E rapid identification system and conventional phenotypic methods.
Tinsley, John W, D A Austin, Lyndon, Alastair R, Lyndon, A. R.; id_orcid, A R Lyndon, Austin, Brian, Austin, Dawn A, Austin, D. A., J W Tinsley, Tinsley, J. W., B Austin, Austin, B.   +11 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Mitochondrial heteroplasmy improves risk prediction for myeloid neoplasms

Nature Communications
Clonal hematopoiesis of indeterminate potential is the primary pathogenic risk factor for myeloid neoplasms, while heteroplasmy (mutations in a subset of cellular mitochondrial DNA) is another marker of clonal expansion associated with hematological ...
Yun Soo Hong, Sergiu Pasca, Wen Shi, Daniela Puiu, Nicole J. Lake, Monkol Lek, Meng Ru, Megan L. Grove, Anna Prizment, Corinne E. Joshu, Elizabeth A. Platz, Eliseo Guallar, Dan E. Arking, Lukasz P. Gondek   +13 more
doaj   +1 more source

Integrative single-cell multi-omics of CD19-CARpos and CARneg T cells suggest drivers of immunotherapy response in B cell neoplasias

Cell Reports Medicine
Summary: The impact of phenotypic, clonal, and functional heterogeneity of chimeric antigen receptor (CAR)-T cells on clinical outcome remains understudied.
Mercedes Guerrero-Murillo, Aina Rill-Hinarejos, Juan L. Trincado, Alex Bataller, Valentín Ortiz-Maldonado, Daniel Benítez-Ribas, Marta Español-Rego, E Azucena González-Navarro, Nuria Martínez-Cibrián, Doménica Marchese, Lourdes Martín-Martín, Alejandro Martín García-Sancho, Susana Rives, Holger Heyn, Manel Juan, Álvaro Urbano-Ispizúa, Julio Delgado, Alberto Orfao, Elisabetta Mereu, Clara Bueno, Pablo Menendez   +20 more
doaj   +1 more source