CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein [PDF]
, 2019 The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling.
Babaei, Milad +8 more
core +1 more source
Genetic heterogeneity of induced pluripotent stem cells: results from 24 clones derived from a single C57BL/6 mouse. [PDF]
PLoS ONE, 2015 Induced pluripotent stem cells (iPSCs) have tremendous potential as a tool for disease modeling, drug testing, and other applications. Since the generation of iPSCs "captures" the genetic history of the individual cell that was reprogrammed, iPSC clones (
Cheng Li +11 more
doaj +1 more source
Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling. [PDF]
, 2014 One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment.
Bonora, M +9 more
core +3 more sources
Haematologica, 2010 Background Clones of glycosylphosphatidylinositol-anchor protein-deficient cells are characteristic in paroxysmal nocturnal hemoglobinuria and are present in about 40–50% of patients with severe aplastic anemia.
Phillip Scheinberg +3 more
doaj +1 more source
Di-san junyi daxue xuebao, 2020 Objective To study the effect of down-regulation of long non-coding RNA (lncRNA) HOXA cluster antisense RNA 2 (HOXA-AS2)/miR-20a pathway on apoptosis and invasion of skin squamous cell carcinoma cells in vitro.
XU Wen, LIU Xuhong, LIU Hua
doaj +1 more source
Platelets promote macrophage polarization toward pro-inflammatory phenotype and increase survival of septic mice [PDF]
, 2019 We investigated the contribution of human platelets to macrophage effector properties in the presence of lipopolysaccharide (LPS), as well as the beneficial effects and time frame for platelet transfusion in septic animals.
Carestia, Agostina +9 more
core +1 more source
Transient down-regulation of beta1 integrin subtypes on kidney carcinoma cells is induced by mechanical contact with endothelial cell membranes [PDF]
, 2007 Adhesion molecules of the integrin beta1 family are thought to be involved in the malignant progression renal cell carcinoma (RCC). Still, it is not clear how they contribute to this process.
Achiwa H +10 more
core +1 more source
Targeting interleukin-1β protects from aortic aneurysms induced by disrupted transforming growth factor β signaling [PDF]
, 2017 Aortic aneurysms are life-threatening conditions with effective treatments mainly limited to emergency surgery or trans-arterial endovascular stent grafts, thus calling for the identification of specific molecular targets.
Bizzotto, Dario +13 more
core +1 more source
ReMixT: clone-specific genomic structure estimation in cancer
Genome Biology, 2017 Somatic evolution of malignant cells produces tumors composed of multiple clonal populations, distinguished in part by rearrangements and copy number changes affecting chromosomal segments. Whole genome sequencing mixes the signals of sampled populations,
Andrew W. McPherson +10 more
doaj +1 more source
Phenotypic evolution in microalgae: a dramatic morphological shift in Dictyosphaerium chlorelloides (Chlorophyta) after exposure to TNT [PDF]
, 2006 Phenotypic evolution in microalgae: a dramatic morphological shift in Dictyosphaerium chlorelloides (Chlorophyta) after exposure to TNT. The occurrence of rapid morphological evolution in the microalga Dictyosphaerium chlorelloides (Chlorophyta) was ...
Costas, Eduardo +3 more
core +3 more sources