Results 41 to 50 of about 822,631 (292)

In vivo evidence for quasispecies distributions in the bovine respiratory syncytial virus genome [PDF]

, 2007
We analyzed the genetic evolution of bovine respiratory syncytial virus (BRSV) isolate W2-00131, from its isolation in bovine turbinate (BT) cells to its inoculation in calves.
Bonnet, Marion, Deplanche, Martine, Lemaire, Mylène, Meyer, Gilles, Mirandette, Carole, Schelcher, François   +5 more
core   +1 more source

Molecular cloning of schistosome genes

Parasitology, 1986
As part of an integrated programme investigating human schistosomiasis, work which involves epidemiological surveys and detailed immunological studies as well as biochemical investigations, we have, over the last three years, been cloning schistosome genes in a variety of plasmid and lambda vector systems.
D W, Taylor, J S, Cordingley, D W, Dunne, K S, Johnson, W J, Haddow, C E, Hormaeche, V, Nene, A E, Butterworth   +7 more
openaire   +2 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

New Clox Systems for rapid and efficient gene disruption in Candida albicans [PDF]

, 2014
Acknowledgements: We are grateful to Janet Quinn, Lila Kastora, Joanna Potrykus, Michelle Leach, and others for sharing their experiences with the Clox cassettes.
Ballou, Elizabeth R, Bohovych, Iryna, Brown, Alistair J P, Childers, Delma S, Gow, Neil A. R., Odds, Frank C, Shahana, Shahida   +6 more
core   +4 more sources

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment

Drug Design, Development and Therapy, 2020
Hassan Dana, 1, 2,* Ghanbar Mahmoodi Chalbatani, 1,* Elahe Gharagouzloo, 1 Seyed Rouhollah Miri, 1 Fereidoon Memari, 1 Reza Rasoolzadeh, 3 Mohammad Reza Zinatizadeh, 3 Peyman Kheirandish Zarandi, 3 Vahid Marmari 2 1Cancer Research Center, Cancer ...
Dana H, Mahmoodi Chalbatani G, Gharagouzloo E, Miri SR, Memari F, Rasoolzadeh R, Zinatizadeh MR, Kheirandish Zarandi P, Marmari V   +8 more
doaj  

A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

BMC Research Notes, 2012
Background Molecular cloning of DNA fragments >5 kbp is still a complex task. When no genomic DNA library is available for the species of interest, and direct PCR amplification of the desired DNA fragment is unsuccessful or results in an incorrect ...
Orlova Nadezhda A, Orlov Alexandre V, Vorobiev Ivan I   +2 more
doaj   +1 more source

Efficient Parallel Algorithm for Statistical Ion Track Simulations in Crystalline Materials

, 2008
We present an efficient parallel algorithm for statistical Molecular Dynamics simulations of ion tracks in solids. The method is based on the Rare Event Enhanced Domain following Molecular Dynamics (REED-MD) algorithm, which has been successfully applied
Beardmore, Beardmore, Biersack, Brandt, Byoungseon Jeon, Cai, Cai, Elteckov, Firsov, Gropp, Kang, Kishinevskii, Niels Grønbech-Jensen, Robinson, Sillanpää, Tasch, Van Brutzel, Verlet, Ziegler, Ziegler   +19 more
core   +1 more source

Characterization, cloning and immunogenicity of antigens released by transforming cercariae of Schistosoma mansoni [PDF]

, 2000
A schistosome infection is initiated when the parasite penetrates the skin of a susceptible host. Relatively large quantities of protein are released by transforming cercariae compared to later larval stages.
Coulson, P.S., Harrop, R., Jennings, N., Moutford, A.P., Wilson, R.A.   +4 more
core   +1 more source

Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]

, 2020
Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P, Ibrahim, AEC, Ma, J, Van Dolleweerd, CJ   +3 more
core   +1 more source