Results 41 to 50 of about 5,041,968 (259)
Molecular cloning of cat interleukin-12 [PDF]
Immunogenetics, 1997 The recently discovered interleukin-12 (IL-12) is a heterodimeric 70000 Mr cytokine composed of a disulphide-bonded 35000 Mr (p35) subunit and a 40000 Mr (p40) subunit. Produced mainly by activated monocytes/macrophages and B cells, it enhances NK/LAK cell cytolytic activity and stimulates cytotoxic T-cell responses and IFN-γ production.
Horzinek, M.C. +3 more
openaire +4 more sources
A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
BMC Biotechnology, 2010 Background The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences.
Nørholm Morten HH
doaj +1 more source
Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice
Czech Journal of Genetics and Plant Breeding, 2022 Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism,
Yu Li +6 more
doaj +1 more source
Quantum storage and cloning of light states in EIT-like medium [PDF]
Int. J. of Modern Physics B, 20 (2006) 1593-1605, 2006 In the paper we consider a new approach for storage and cloning of quantum information by three level atomic (molecular) systems in the presence of the electromagnetically induced transparency (EIT) effect. For that, the various schemes of transformation into the bright and dark polaritons for quantum states of optical field in the medium are proposed.
arxiv +1 more source
Microbiology, 1996 Relatively limited information about promoter structures in Corynebacterium glutamicum has been available until now. With the aim of isolating and characterizing such transcription initiation signals, random Sau3A fragments of C.
M. Pátek +3 more
semanticscholar +1 more source
Stem Cell Research & Therapy, 2019 Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu +13 more
doaj +1 more source
Glycerol kinase of Trypanosoma brucei. Cloning, molecular characterization and mutagenesis.
European Journal of Biochemistry, 2000 Trypanosoma brucei contains two tandemly arranged genes for glycerol kinase. The downstream gene was analysed in detail. It contains an ORF for a polypeptide of 512 amino acids. The polypeptide has a calculated molecular mass of 56 363 Da and a pI of 8.6.
Ivica Králová +3 more
semanticscholar +1 more source
Simplifying recombinant protein production: Combining Golden Gate cloning with a standardized protein purification scheme [PDF]
arXiv, 2023 Recombinant protein production is pivotal in molecular biology, enabling profound insights into cellular processes through biophysical, biochemical, and structural analyses of the purified samples. The demand for substantial biomolecule quantities often presents challenges, particularly for eukaryotic proteins.
arxiv
Journal of Biological Chemistry, 1997 A full-length cDNA of the accessory (beta) subunit of mitochondrial DNA polymerase from Drosophila embryos has been obtained, and its nucleotide sequence was determined.
Yuxun Wang, C. Farr, L. Kaguni
semanticscholar +1 more source
Molecular cloning and expression of human caldecrin [PDF]
FEBS Letters, 1996 Earlier we reported the primary structure of serum calcium‐decreasing factor (caldecrin) from rat pancreas, a protein which is considered to be a member of the elastase family. In this report, we describe the isolation of the two homologous cDNA clones encoding caldecrin from human pancreas, the structures of which are identical except for one base and
Mineko Tomomura +7 more
openaire +3 more sources