Results 41 to 50 of about 5,018,281 (306)
Glycerol kinase of Trypanosoma brucei. Cloning, molecular characterization and mutagenesis.
European Journal of Biochemistry, 2000 Trypanosoma brucei contains two tandemly arranged genes for glycerol kinase. The downstream gene was analysed in detail. It contains an ORF for a polypeptide of 512 amino acids. The polypeptide has a calculated molecular mass of 56 363 Da and a pI of 8.6.
Ivica Králová +3 more
semanticscholar +1 more source
CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method. [PDF]
, 2017 Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is a powerful tool to manipulate the genome with extraordinary simplicity and speed.
Chen, Chao +6 more
core +3 more sources
arXiv, 2022 We develop a new general framework for algebras and clones, called Universal Clone Algebra. Algebras and clones of finitary operations are to Universal Algebra what t-algebras and clone algebras are to Universal Clone Algebra. Clone algebras have been recently introduced to found a one-sorted, purely algebraic theory of clones, while t-algebras are ...
arxiv
Journal of Biological Chemistry, 1997 A full-length cDNA of the accessory (beta) subunit of mitochondrial DNA polymerase from Drosophila embryos has been obtained, and its nucleotide sequence was determined.
Yuxun Wang, C. Farr, L. Kaguni
semanticscholar +1 more source
Molecular cloning and characterization of human endothelial nitric oxide synthase [PDF]
, 1992 The constitutive calcium/calmodulin-dependent nitric oxide (NO) synthase expressed in vascular endothelium shares common biochemical and pharmacologic properties with neuronal NO synthase.
Ignarro +21 more
core +2 more sources
STAR Protocols, 2022 Summary: Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures.
Ana Luiza Drumond-Bock +4 more
doaj
Cancer systems biology in the genome sequencing era: Part 2, evolutionary dynamics of tumor clonal networks and drug resistance [PDF]
, 2014 A tumor often consists of multiple cell subpopulations (clones). Current chemo-treatments often target one clone of a tumor. Although the drug kills that clone, other clones overtake it and the tumor reoccurs. Genome sequencing and computational analysis allows to computational dissection of clones from tumors, while singe-cell genome sequencing ...
arxiv +1 more source
Controlled Secret Sharing Protocol using a Quantum Cloning Circuit [PDF]
Quant.Inf.Proc. 13, 2071 (2014), Springer, 2014 We demonstrate the possibility of controlling the success probability of a secret sharing protocol using a quantum cloning circuit. The cloning circuit is used to clone the qubits containing the encoded information and {\em en route} to the intended receipients. The success probability of the protocol depends on the cloning parameters used to clone the
arxiv +1 more source
Molecular cloning and characterization of B-cadherin, a novel chick cadherin. [PDF]
, 1991 Calcium-dependent cell-cell adhesion is mediated in large part by a set of homologous integral membrane glycoproteins termed cadherins. In this report, antibodies to conserved domains in previously described cadherins have been used to isolate cDNAs ...
Napolitano, EW +3 more
core +2 more sources
Cancer systems biology in the genome sequencing era: Part 1, dissecting and modeling of tumor clones and their networks [PDF]
, 2014 Recent tumor genome sequencing confirmed that one tumor often consists of multiple cell subpopulations (clones) which bear different, but related, genetic profiles such as mutation and copy number variation profiles. Thus far, one tumor has been viewed as a whole entity in cancer functional studies.
arxiv +1 more source