Results 51 to 60 of about 5,041,968 (259)
STAR Protocols, 2022 Summary: Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures.
Ana Luiza Drumond-Bock +4 more
doaj
Cancer systems biology in the genome sequencing era: Part 2, evolutionary dynamics of tumor clonal networks and drug resistance [PDF]
, 2014 A tumor often consists of multiple cell subpopulations (clones). Current chemo-treatments often target one clone of a tumor. Although the drug kills that clone, other clones overtake it and the tumor reoccurs. Genome sequencing and computational analysis allows to computational dissection of clones from tumors, while singe-cell genome sequencing ...
arxiv +1 more source
arXiv, 2022 We develop a new general framework for algebras and clones, called Universal Clone Algebra. Algebras and clones of finitary operations are to Universal Algebra what t-algebras and clone algebras are to Universal Clone Algebra. Clone algebras have been recently introduced to found a one-sorted, purely algebraic theory of clones, while t-algebras are ...
arxiv
Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning [PDF]
, 2010 Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Balhana, Ricardo +4 more
core +2 more sources
Cancer systems biology in the genome sequencing era: Part 1, dissecting and modeling of tumor clones and their networks [PDF]
, 2014 Recent tumor genome sequencing confirmed that one tumor often consists of multiple cell subpopulations (clones) which bear different, but related, genetic profiles such as mutation and copy number variation profiles. Thus far, one tumor has been viewed as a whole entity in cancer functional studies.
arxiv +1 more source
Controlled Secret Sharing Protocol using a Quantum Cloning Circuit [PDF]
Quant.Inf.Proc. 13, 2071 (2014), Springer, 2014 We demonstrate the possibility of controlling the success probability of a secret sharing protocol using a quantum cloning circuit. The cloning circuit is used to clone the qubits containing the encoded information and {\em en route} to the intended receipients. The success probability of the protocol depends on the cloning parameters used to clone the
arxiv +1 more source
Expression capable library for studies of Neisseria gonorrhoeae, version 1.0 [PDF]
, 2005 Background The sexually transmitted disease, gonorrhea, is a serious health problem in developed as well as in developing countries, for which treatment continues to be a challenge.
Altherr, Michael R. +18 more
core +3 more sources
Arguments for Human Therapeutic Cloning
Biomolecules & Biomedicine, 2008 For over 30 years, many Western governments have regulated scientific research involving human subjects. According to Knoppers implementation of regulation followed a long and checkered history of research abuse.
Krešimir Pavelić
doaj +1 more source
Automation and miniaturization of Golden Gate DNA assembly reactions using acoustic dispensers [PDF]
arXiv, 2023 Golden Gate cloning has become one of the most popular DNA assembly techniques. Its modular and hierarchical structure allows the construction of complex DNA fragments. Over time, Golden Gate cloning allows for the creation of a repository of reusable parts, reducing the cost of frequent sequence validation.
arxiv
Molecular cloning of the genome of human spumaretrovirus [PDF]
Gene, 1987 DNA of human spumaretrovirus (HSRV) was cloned from both cDNA and from viral DNA into phage lambda and bacterial plasmid vectors. The recombinant plasmids harboring viral DNA were characterized by Southern blot hybridization and restriction mapping. Physical maps were constructed from cDNA and found to be colinear with the restriction maps obtained ...
Rethwilm, Axel +4 more
openaire +4 more sources