Results 51 to 60 of about 759,664 (294)

The Alliance for Cellular Signaling Plasmid Collection: A Flexible Resource for Protein Localization Studies and Signaling Pathway Analysis [PDF]

, 2007
Cellular responses to inputs that vary both temporally and spatially are determined by complex relationships between the components of cell signaling networks.
Bryan, Heather, Chandy, Grischa, Chang, Mi Sook, Coutre, Sam, Dhandapani, Kavitha, Driver, Adrienne, Fraser, Iain D. C., Fung, Eileen, Gehrig, Elizabeth, Kivork, Christine, Lee, Genie, Liu, Jamie, Lyon, Stephen, Maer, Andreia, Meyer, Tobias, Mi, Qingli, Ning, Yuhong, O'Rourke, Nancy, Park, Wei Sun, Randhawa, Baljinder, Santat, Leah, Saunders, Brian, Simon, Melvin I., Subramaniam, Shankar, Verghese, Mary, Wall, Estelle, Whalen, James, Zavzavadjian, Joelle R., Zhu, Xiaocui   +28 more
core   +1 more source

Characterization, cloning and immunogenicity of antigens released by transforming cercariae of Schistosoma mansoni [PDF]

, 2000
A schistosome infection is initiated when the parasite penetrates the skin of a susceptible host. Relatively large quantities of protein are released by transforming cercariae compared to later larval stages.
Coulson, P.S., Harrop, R., Jennings, N., Moutford, A.P., Wilson, R.A.   +4 more
core   +1 more source

Molecular cloning of HCV and clinical application [PDF]

FEMS Microbiology Reviews, 1994
Fifty-five clones encoding epitopes of HCV were isolated from Japanese patients. Their amino acid homology (AAH) to the sequence of prototype (HCV-1) ranged from 47% to 94%. These sequences cover 60% of the HCV genome lacking M/E and NS2 regions suggesting a very low or lacking immunogenicity for these regions.
openaire   +4 more sources

Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation

FEBS Letters, EarlyView.
The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt, Marcus S. Münchhoff, Jacqueline Krohn, Philip M. Palarz, Manuel H. Taft, Johannes N. Greve, Nataliya Di Donato, Falk F. R. Buettner, Dietmar J. Manstein   +8 more
wiley   +1 more source

TLTC, a T5 exonuclease–mediated low-temperature DNA cloning method

Frontiers in Bioengineering and Biotechnology, 2023
Molecular cloning is used in a wide variety of biological and medical research. Here, we developed a rapid and efficient DNA-assembling method for routine laboratory work.
Fang Yu, Xia Li, Fei Wang, Yang Liu, Chao Zhai, Wenqiang Li, Lixin Ma, Wanping Chen, Wanping Chen   +8 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Bioinformatic Analysis in Designing Mega-primer in Overlap Extension PCR Cloning (OEPC) Technique

JOIV: International Journal on Informatics Visualization, 2021
Bioinformatics has developed into an application tool for basic and applied research in the biomedical and biotechnology field. Polymerase Chain Reaction (PCR) is a common technique in the molecular area that has always involved bioinformatics science ...
- Mardalisa, Sony Suhandono, Novi Yanti, Fazrol Rozi, Fitri Nova, - Primawati   +5 more
doaj   +1 more source

BacS: An Abundant Bacteroid Protein in \u3cem\u3eRhizobium etli\u3c/em\u3e Whose Expression Ex Planta Requires \u3cem\u3enifA\u3c/em\u3e [PDF]

, 2003
Rhizobium etli CFN42 bacteroids from bean nodules possessed an abundant 16-kDa protein (BacS) that was found in the membrane pellet after cell disruption. This protein was not detected in bacteria cultured in tryptone-yeast extract.
Davila, Guillermo, Jahn, Olivia Jeanne, Noel, K. Dale, Romero, David   +3 more
core   +1 more source

Sequence-specific protein aggregation generates defined protein knockdowns in plants [PDF]

, 2016
Protein aggregation is determined by short (5-15 amino acids) aggregation-prone regions (APRs) of the polypeptide sequence that self-associate in a specific manner to form beta-structured inclusions.
Aesaert, Stijn, Betti, Camilla, Coutuer, Silvie, De Rycke, Riet, De Smet, Frederik, Gallardo, Rodrigo, Inzé, Dirk, Mishev, Kiril, Rombaut, Debbie, Rousseau, Frederic, Russinova, Eugenia, Schymkowitz, Joost, Van Breusegem, Frank, Van Lijsebettens, Maria, Vanhoutte, Isabelle, Vuylsteke, Marnik, Xu, Jie   +16 more
core   +2 more sources

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source