Automation and miniaturization of Golden Gate DNA assembly reactions using acoustic dispensers [PDF]
Golden Gate cloning has become one of the most popular DNA assembly techniques. Its modular and hierarchical structure allows the construction of complex DNA fragments. Over time, Golden Gate cloning allows for the creation of a repository of reusable parts, reducing the cost of frequent sequence validation.
arxiv
Quantum Cloning of Mixed States in Symmetric Subspace [PDF]
Quantum cloning machine for arbitrary mixed states in symmetric subspace is proposed. This quantum cloning machine can be used to copy part of the output state of another quantum cloning machine and is useful in quantum computation and quantum information. The shrinking factor of this quantum cloning achieves the well-known upper bound.
arxiv +1 more source
Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research. [PDF]
Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-coding ...
Mostafa Aboulela+7 more
doaj +1 more source
How to compute the thermodynamics of a glass using a cloned liquid [PDF]
The recently proposed strategy for studying the equilibrium thermodynamics of the glass phase using a molecular liquid is reviewed and tested in details on the solvable case of the $p$-spin model. We derive the general phase diagram, and confirm the validity of this procedure.
arxiv +1 more source
Cloning of a human parvovirus by molecular screening of respiratory tract samples.
The identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR ...
semanticscholar +1 more source
Arguments for Human Therapeutic Cloning
For over 30 years, many Western governments have regulated scientific research involving human subjects. According to Knoppers implementation of regulation followed a long and checkered history of research abuse.
Krešimir Pavelić
doaj +1 more source
Local cloning of two product states [PDF]
Local quantum operations and classical communication (LOCC) put considerable constraints on many quantum information processing tasks such as cloning and discrimination.
Mingsheng Ying+3 more
core +2 more sources
Molecular Cloning of Gonadotropin Hormones I (Gth-i) and II (Gth-ii) Genes in the Hard-lipped Barb (Osteochillus Hasseltii) and the Effect of Photoperiods on the Genes Expression [PDF]
Photoperiods is one of the factors that influence regulation on endocrine gland in producing hormones necessary for gonadal growth and development, gametogenesis and reproductive cycles in fish reproduction. This study clones the gonadotropin hormones I (
Kawaichi, M. (Masashi)+4 more
core
Expression capable library for studies of Neisseria gonorrhoeae, version 1.0 [PDF]
Background The sexually transmitted disease, gonorrhea, is a serious health problem in developed as well as in developing countries, for which treatment continues to be a challenge.
Altherr, Michael R.+18 more
core +3 more sources
In vivo IL‐10 produced by tissue‐resident tolDC is involved in maintaining/inducing tolerance. Depending on the agent used for ex vivo tolDC generation, cells acquire common features but prime T cells towards anergy, FOXP3+ Tregs, or Tr1 cells according to the levels of IL‐10 produced. Ex vivo‐induced tolDC were administered to patients to re‐establish/
Konstantina Morali+3 more
wiley +1 more source