Results 51 to 60 of about 538,381 (248)

How advancements in molecular biology impact education and training

Journal of Microbiology & Biology Education
Molecular biology, broadly defined as the investigation of complex biomolecules in the laboratory, is a rapidly advancing field and as such the technologies available to investigators are constantly evolving.
Rheanna E. Walther, Michael Hrabak, Douglas A. Bernstein   +2 more
doaj   +1 more source

Cloning of cryptogein gene and construction of its plant expression vector

浙江大学学报. 农业与生命科学版, 2002
The cryptogein gene was obtained by PCR amplification from genomic DNA of Phytophthora cryptogea and verified by DNA sequencing. A promoter of a rice phenylalanine ammonia-lyase (PAL) gene was selected to control the expression of cryptogein gene ...
JIANG Dong-hua, KONG Shi-hai, GUO Ze-Jian   +2 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Unveiling mungbean yellow mosaic virus: molecular insights and infectivity validation in mung bean (Vigna radiata) via infectious clones

Frontiers in Plant Science
Yellow mosaic disease (YMD) with typical symptoms of alternating bright yellow to green patches associated with stunting, downward cupping, and wrinkling has been observed in mung bean on agricultural farms in Coimbatore, Tamil Nadu, India.
Madhumitha Balasubramaniam, Madhumitha Balasubramaniam, Tamilnayagan Thangavel, Karupiah Eraivan Arutkani Aiyanathan, Sakthi Ambothi Rathnasamy, Veera Ranjani Rajagopalan, Mohankumar Subbarayalu, Senthil Natesan, Selvaraju Kanagarajan, Selvaraju Kanagarajan, Raveendran Muthurajan, Sudha Manickam   +11 more
doaj   +1 more source

Cloning and analysis of promoter of Paralichthys olivaceus Follistatin gene

浙江大学学报. 农业与生命科学版, 2007
In order to study the tissue expression character of Follistatin gene in marine fish, the promoter of Paralichthys olivaceus Follistatin gene was isolated by PCR, and the sequence was analyzed; the function of Follistatin gene on the development of ...
LIU Qing-hua, TAN Xun-gang, XU Yong-li, ZHANG Pei-jun, ZHANG Yu-qing   +4 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Molecular cloning, sequencing, expression and purification of Alkhumra hemorrhagic fever virus capsid protein in Saudi Arabia

Journal of Infection in Developing Countries
Introduction: Alkhumra hemorrhagic fever virus (AHFV) is a newly discovered virus in the Flaviviridae family. It was discovered in 1995 among animal handlers in Saudi Arabia.
Ahmed M Hassan, Nada M Aljuaid, Magdah A Ganash, Sayed S Sohrab, Esam I Azhar   +4 more
doaj   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Recombinant Bile Salt Hydrolase Enhances the Inhibition Efficiency of Taurodeoxycholic Acid against Clostridium perfringens Virulence

Pathogens
Clostridium perfringens is the main pathogen of chicken necrotic enteritis (NE) causing huge economic losses in the poultry industry. Although dietary secondary bile acid deoxycholic acid (DCA) reduced chicken NE, the accumulation of conjugated tauro-DCA
Tahrir Alenezi, Bilal Alrubaye, Ying Fu, Janashrit Shrestha, Samar Algehani, Hong Wang, Rohana Liyanage, Xiaolun Sun   +7 more
doaj   +1 more source

Perspectives in educating molecular pathologists on liquid biopsy: Toward integrative, equitable, and decentralized precision oncology

Molecular Oncology, EarlyView.
Liquid biopsy enables minimally invasive, real‐time molecular profiling through analysis of circulating biomarkers in biological fluids. This Perspective highlights the importance of training pathologists through integrative educational programs, such as the European Masters in Molecular Pathology, to ensure effective and equitable implementation of ...
Marius Ilié, Umberto Malapelle, Catherine Alix‐Panabières, Claus Lindbjerg Andersen, Sandra Chlebowski, Vivien Lake, Caroline Lacoux, Virginie Lespinet‐Fabre, Olivier Bordone, Simon Heeke, Christophe Bontoux, Ellen Heitzer, Klaus Pantel, Paul Hofman   +13 more
wiley   +1 more source