Results 61 to 70 of about 695,609 (307)

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Cloning, Clones and Clonal Disease

Journal of the Royal College of Physicians of London, 2000
In the past, cloning has been familiar to plant breeders because many plants can be easily reproduced in this way, bypassing the lengthy process of cross-fertilisation. Recently, the concept of cloning has become popular in human biology and medicine on two accounts.
openaire   +2 more sources

Molecular characterization and tissue expression profile of the Dnmts gene family in pig

Journal of Integrative Agriculture, 2017
DNA methyltransferases (Dnmts) comprise a family of proteins which involved in the establishment and maintenance of DNA methylation patterns. In pig, the molecular characterization and tissue expression profile of Dnmt gene family are not clear. To solve
Zong-gang LUO, Kai ZHANG, Lei CHEN, Yuan-xin YANG, Peng-hui FU, Ke-tian WANG, Ling WANG, Ming-zhou LI, Xue-wei LI, Fu-yuan ZUO, Jin-yong WANG   +10 more
doaj   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Cloning, bioinformatics analysis and expression of the cysteine dioxygenase type 1 (CDO1) gene in domestic yak

Frontiers in Veterinary Science
IntroductionThe CDO1 gene is an important gene in the taurine synthesis pathway and has been observed to have high expression in ovaries of female mammals.
Yuxin Fu, Yuxin Fu, Yuxin Fu, Jiuru Yan, Jiuru Yan, Jiuru Yan, Lan Lan, Huizhu Zhang, Huizhu Zhang, Huizhu Zhang, Peng Wang, Yaying Wang, Yaying Wang, Xianrong Xiong, Xianrong Xiong, Xianrong Xiong, Jian Li, Jian Li, Jian Li, Honghong He, Honghong He, Honghong He   +21 more
doaj   +1 more source

Comparing the paths of Hedgehog release in artificial and natural settings

FEBS Letters, EarlyView.
Hh morphogens require solubilization factors to overcome their tight association with the plasma membrane and signal to distant cells, but the precise functions of these factors are controversial. Here, we use published in vivo observations as the most relevant evidence to test their suggested functions and discuss contradictions and gaps between in ...
Janna Puschmann, Kay Grobe
wiley   +1 more source

Birth of clones of the world’s first cloned dog [PDF]

Scientific Reports, 2017
AbstractAnimal cloning has gained popularity as a method to produce genetically identical animals or superior animals for research or industrial uses. However, the long-standing question of whether a cloned animal undergoes an accelerated aging process is yet to be answered. As a step towards answering this question, we compared longevity and health of
Kim, Min Jung, Oh, Hyun Ju, Kim, Geon A., Setyawan, Erif Maha Nugraha, Bin Choi, Yoo, Lee, Seok Hee, Petersen-Jones, Simon M., Ko, CheMyong J., Lee, Byeong Chun   +8 more
openaire   +3 more sources

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Human Cloning Research. National Academy of Sciences Forum (2001 August 7) (Part 1)

, 2001
The scientists provided background information on a variety of topics, including embryology, scientific issues underlying cloning, and the role of mitochondria.
Producer: National Cable Satellite Corporation. C-SPAN
core  

Cloning of cryptogein gene and construction of its plant expression vector

浙江大学学报. 农业与生命科学版, 2002
The cryptogein gene was obtained by PCR amplification from genomic DNA of Phytophthora cryptogea and verified by DNA sequencing. A promoter of a rice phenylalanine ammonia-lyase (PAL) gene was selected to control the expression of cryptogein gene ...
JIANG Dong-hua, KONG Shi-hai, GUO Ze-Jian   +2 more
doaj   +1 more source