Results 61 to 70 of about 687,759 (305)

Molecular characterization and tissue expression profile of the Dnmts gene family in pig

Journal of Integrative Agriculture, 2017
DNA methyltransferases (Dnmts) comprise a family of proteins which involved in the establishment and maintenance of DNA methylation patterns. In pig, the molecular characterization and tissue expression profile of Dnmt gene family are not clear. To solve
Zong-gang LUO, Kai ZHANG, Lei CHEN, Yuan-xin YANG, Peng-hui FU, Ke-tian WANG, Ling WANG, Ming-zhou LI, Xue-wei LI, Fu-yuan ZUO, Jin-yong WANG   +10 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems. [PDF]

FEBS Open Bio
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhu Z, Liu Y, Qin L, Yu F, Wang Q, Liu Y.   +5 more
europepmc   +2 more sources

Cloning, Clones and Clonal Disease

Journal of the Royal College of Physicians of London, 2000
In the past, cloning has been familiar to plant breeders because many plants can be easily reproduced in this way, bypassing the lengthy process of cross-fertilisation. Recently, the concept of cloning has become popular in human biology and medicine on two accounts.
openaire   +2 more sources

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Cloning, bioinformatics analysis and expression of the cysteine dioxygenase type 1 (CDO1) gene in domestic yak

Frontiers in Veterinary Science
IntroductionThe CDO1 gene is an important gene in the taurine synthesis pathway and has been observed to have high expression in ovaries of female mammals.
Yuxin Fu, Yuxin Fu, Yuxin Fu, Jiuru Yan, Jiuru Yan, Jiuru Yan, Lan Lan, Huizhu Zhang, Huizhu Zhang, Huizhu Zhang, Peng Wang, Yaying Wang, Yaying Wang, Xianrong Xiong, Xianrong Xiong, Xianrong Xiong, Jian Li, Jian Li, Jian Li, Honghong He, Honghong He, Honghong He   +21 more
doaj   +1 more source

Epigenetic reprogramming of lineage switching in cancer

FEBS Letters, EarlyView.
Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı, Burcu Akman, Ezgi Bağırsakçı, Nese Atabey, Serap Erkek‐Ozhan   +4 more
wiley   +1 more source

Birth of clones of the world’s first cloned dog [PDF]

Scientific Reports, 2017
AbstractAnimal cloning has gained popularity as a method to produce genetically identical animals or superior animals for research or industrial uses. However, the long-standing question of whether a cloned animal undergoes an accelerated aging process is yet to be answered. As a step towards answering this question, we compared longevity and health of
Kim, Min Jung, Oh, Hyun Ju, Kim, Geon A., Setyawan, Erif Maha Nugraha, Bin Choi, Yoo, Lee, Seok Hee, Petersen-Jones, Simon M., Ko, CheMyong J., Lee, Byeong Chun   +8 more
openaire   +3 more sources

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Cloning of cryptogein gene and construction of its plant expression vector

浙江大学学报. 农业与生命科学版, 2002
The cryptogein gene was obtained by PCR amplification from genomic DNA of Phytophthora cryptogea and verified by DNA sequencing. A promoter of a rice phenylalanine ammonia-lyase (PAL) gene was selected to control the expression of cryptogein gene ...
JIANG Dong-hua, KONG Shi-hai, GUO Ze-Jian   +2 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source