Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum. [PDF]
Microb Cell Fact, 2021 Background The intracellular ATP level is an indicator of cellular energy state and plays a critical role in regulating cellular metabolism. Depletion of intracellular ATP in (facultative) aerobes can enhance glycolysis, thereby promoting end product ...
Dai Z +5 more
europepmc +2 more sources
Design and validation of a multiplex PCR method for the simultaneous quantification of Clostridium acetobutylicum, Clostridium carboxidivorans and Clostridium cellulovorans [PDF]
Scientific Reports, 2023 Co-cultures of clostridia with distinct physiological properties have emerged as an alternative to increase the production of butanol and other added-value compounds from biomass.
Laura Feliu-Paradeda +2 more
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Chromosomal integration of the pSOL1 megaplasmid of Clostridium acetobutylicum for continuous and stable advanced biofuels production. [PDF]
Nat MicrobiolBiofuel production by Clostridium acetobutylicum is compromised by strain degeneration due to loss of its pSOL1 megaplasmid. Here we used engineering biology to stably integrate pSOL1 into the chromosome together with a synthetic isopropanol pathway.
Ehsaan M +5 more
europepmc +2 more sources
Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in Clostridium acetobutylicum. Here, we knocked down the atpG gene encoding the ATPase gamma subunit in C. acetobutylicum ATCC 824 using a mobile group II intron system and
Yu-Sin Jang +7 more
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Effects of Carbon Ion Beam Irradiation on Butanol Tolerance and Production of Clostridium acetobutylicum. [PDF]
Front Microbiol, 2020 Clostridium acetobutylicum (C. acetobutylicum) has considerable potential for use in bioenergy development. Owing to the repeated use of traditional mutagenesis methods, the strains have developed a certain tolerance.
Gao Y +6 more
europepmc +2 more sources
Pemanfaatan Serbuk Gergaji Menjadi Biobutanol dengan Hidrolisis Selulase dan Fermentasi Bakteri Clostridium Acetobutylicum [PDF]
Jurnal Teknik ITS, 2014 Biobutanol adalah jenis alkohol ikatan C-4 (C4H9OH) yang terbuat dari biomassa. Penelitian ini dilakukan dengan memanfaatkan limbah kayu yang dihasilkan dari proses penggergajian kayu yang mengandung selulosa (55%), hemiselulosa (14%), dan lignin (21 ...
Hayuni Devina Fajariah, Wahyono Hadi
doaj +5 more sources
Identification and regulation of an alternative PTS for disaccharide utilization in Clostridium acetobutylicum [PDF]
Applied and Environmental MicrobiologyClostridium acetobutylicum is an important solventogenic bacterium capable of acetone-butanol-ethanol fermentation by utilizing a variety of carbon sources.
Zhenxing Ren +4 more
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From pre-culture to solvent: current trends in Clostridium acetobutylicum cultivation [PDF]
Applied Microbiology and BiotechnologyThe biological production of butanol via ABE (acetone-butanol-ethanol) fermentation using Clostridium acetobutylicum has a storied history of over 100 years, initially driven by the demand for synthetic rubber during World War I and later for industrial ...
Katharina Oehlenschläger +3 more
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Reassessment of the role of CaCO3 in n-butanol production from pretreated lignocellulosic biomass by Clostridium acetobutylicum. [PDF]
Sci Rep, 2020 In this study, the role of CaCO3 in n-butanol production was further investigated using corn straw hydrolysate (CSH) media by Clostridium acetobutylicum CICC 8016. CaCO3 addition stimulated sugars utilization and butanol production.
Su Z +7 more
europepmc +2 more sources
RRNPP-type quorum sensing affects solvent formation and sporulation in <i>Clostridium acetobutylicum</i>. [PDF]
Microbiology (Reading), 2020 The strictly anaerobic bacterium Clostridium acetobutylicum is well known for its ability to convert sugars into organic acids and solvents, most notably the potential biofuel butanol. However, the regulation of its fermentation metabolism, in particular
Kotte AK +5 more
europepmc +2 more sources