Results 21 to 30 of about 94,691 (233)

A CRISPR/Anti-CRISPR Genome Editing Approach Underlines the Synergy of Butanol Dehydrogenases in Clostridium acetobutylicum DSM 792. [PDF]

Appl Environ Microbiol, 2020
An efficient CRISPR-Cas9 editing tool based on a previous two-plasmid system was developed for Clostridium acetobutylicum and used to investigate the contribution of chromosomal butanol dehydrogenase genes during solventogenesis. Thanks to the control of
Wasels F, Chartier G, Hocq R, Lopes Ferreira N.   +3 more
europepmc   +2 more sources

Separate, separated, and together: the transcriptional program of the Clostridium acetobutylicum-Clostridium ljungdahlii syntrophy leading to interspecies cell fusion [PDF]

mSystems
Syntrophic cocultures (hitherto assumed to be commensalistic) of Clostridium acetobutylicum and Clostridium ljungdahlii, whereby CO2 and H2 produced by the former feed the latter, result in interspecies cell fusion involving large-scale exchange of ...
Noah B. Willis, Eleftherios T. Papoutsakis   +1 more
doaj   +2 more sources

Impact of iron reduction on the metabolism of Clostridium acetobutylicum. [PDF]

Environ Microbiol, 2019
Summary Iron is essential for most living organisms. In addition, its biogeochemical cycling influences important processes in the geosphere (e.g., the mobilization or immobilization of trace elements and contaminants). The reduction of Fe(III) to Fe(II)
List C, Hosseini Z, Lederballe Meibom K, Hatzimanikatis V, Bernier-Latmani R.   +4 more
europepmc   +2 more sources

Gene coexpression network analysis reveals a novel metabolic mechanism of Clostridium acetobutylicum responding to phenolic inhibitors from lignocellulosic hydrolysates. [PDF]

Biotechnol Biofuels, 2020
Background Lignocellulosic biomass is a promising resource of renewable biochemicals and biofuels. However, the presence of inhibitors existing in lignocellulosic hydrolysates (LCH) is a great challenge to acetone-butanol-ethanol (ABE) fermentation by ...
Liu H, Zhang J, Yuan J, Jiang X, Jiang L, Li Z, Yin Z, Du Y, Zhao G, Liu B, Huang D.   +10 more
europepmc   +2 more sources

Cross-talk between engineered Clostridium acetobutylicum and Clostridium ljungdahlii in syntrophic cocultures enhances isopropanol and butanol production [PDF]

Frontiers in Microbiology
There is a need for efficient and sustainable production of essential chemicals such as isopropanol and butanol from renewable sugar feedstocks. Microbial fermentations use glycolysis, and as result, a third of the sugar carbon is lost to CO2 through ...
Jonathan K. Otten, Jonathan K. Otten, John D. Hill, John D. Hill, Noah B. Willis, Noah B. Willis, Joseph Dougherty, Andrew Dalton, Eleftherios T. Papoutsakis, Eleftherios T. Papoutsakis, Eleftherios T. Papoutsakis   +10 more
doaj   +2 more sources

Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins. [PDF]

Appl Environ Microbiol, 2020
Up to this point, assays and methods involving fluorescent reporter proteins were unavailable or limited in Clostridium organisms and other strict anaerobes.
Charubin K, Streett H, Papoutsakis ET.
europepmc   +2 more sources

Continuous Butanol Fermentation of Dilute Acid-Pretreated De-oiled Rice Bran by Clostridium acetobutylicum YM1. [PDF]

Sci Rep, 2019
Continuous fermentation of dilute acid-pretreated de-oiled rice bran (DRB) to butanol by the Clostridium acetobutylicum YM1 strain was investigated. Pretreatment of DRB with dilute sulfuric acid (1%) resulted in the production of 42.12 g/L total sugars ...
Al-Shorgani NKN, Al-Tabib AI, Kadier A, Zanil MF, Lee KM, Kalil MS.   +5 more
europepmc   +2 more sources

Pleiotropic regulation of a glucose-specific PTS in Clostridium acetobutylicum for high-efficient butanol production from corn stover without detoxification. [PDF]

Biotechnol Biofuels, 2019
BackgroundCorn stover (CS) is evaluated as the most favorable candidate feedstock for butanol production via microbial acetone–butanol–ethanol (ABE) fermentation by Clostridium acetobutylicum.
Wu Y, Bai Y, Zhang D, Cheng C, Chen L, Bai F, Xue C.   +6 more
europepmc   +2 more sources

Co-production of solvents and organic acids in butanol fermentation by Clostridium acetobutylicum in the presence of lignin-derived phenolics. [PDF]

RSC Adv, 2019
Co-production of solvents (butanol, acetone, and ethanol) and organic acids (butyrate and acetate) by Clostridium acetobutylicum using lignocellulosic biomass as a substrate could further enlarge the application scope of butanol fermentation.
Luo H, Zheng P, Xie F, Yang R, Liu L, Han S, Zhao Y, Bilal M.   +7 more
europepmc   +2 more sources

Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-forming Clostridium acetobutylicum. [PDF]

Mol Cell Proteomics, 2018
Clostridium acetobutylicum is a strict anaerobic, endospore-forming bacterium, which is used for the production of the high energy biofuel butanol in metabolic engineering. The life cycle of C.
Xu JY, Xu Z, Liu X, Tan M, Ye BC.
europepmc   +2 more sources