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Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum
Applied and Environmental Microbiology, 2022 The majority of the genes present in bacterial genomes remain poorly characterized, with up to one-third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput technique that can help rectify this deficiency.
Claudio Tomi-Andrino +2 more
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Biogas upgrading using Clostridium autoethanogenum for value-added products
Chemical Engineering Journal, 2022Biogas production from anaerobic digestion is a well-established bioprocess for energy generation, nutrient recovery, and valorisation of waste resources. Typical biogas contains 60 % methane and 40 % carbon dioxide (CH4/CO2 of 1.5). Removing or altering carbon dioxide content facilitates biogas use as biomethane due to its impact on the calorific ...
Heffernan, James K. +5 more
openaire +3 more sources
Quantitative analysis of tetrahydrofolate metabolites from clostridium autoethanogenum
Metabolomics, 2018Quantification of tetrahydrofolates (THFs), important metabolites in the Wood-Ljungdahl pathway (WLP) of acetogens, is challenging given their sensitivity to oxygen.To develop a simple anaerobic protocol to enable reliable THFs quantification from bioreactors.Anaerobic cultures were mixed with anaerobic acetonitrile for extraction.
Lemgruber, Renato de Souza Pinto +7 more
openaire +4 more sources
Bioprocess and Biosystems Engineering, 2008
Combined gasification and fermentation technologies can potentially produce biofuels from renewable biomass. Gasification generates synthesis gas consisting primarily of CO, CO(2), H(2), N(2), with smaller amounts of CH(4), NO(x), O(2), C(2) compounds, ash and tars.
Jacqueline L, Cotter +2 more
openaire +2 more sources
Combined gasification and fermentation technologies can potentially produce biofuels from renewable biomass. Gasification generates synthesis gas consisting primarily of CO, CO(2), H(2), N(2), with smaller amounts of CH(4), NO(x), O(2), C(2) compounds, ash and tars.
Jacqueline L, Cotter +2 more
openaire +2 more sources
Effect of Organic Nitrogen Supplements on Syngas Fermentation Using Clostridium autoethanogenum
Biotechnology and Bioprocess Engineering, 2021In this study, the effects of organic nitrogen supplements, such as tryptone and peptone, on microbial growth and ethanol and acetic acid production were investigated to improve ethanol productivity in a Clostridium autoethanogenum culture using syngas as the sole carbon source. The concentration of yeast extract, which is an organic nitrogen source in
Young-Kee Kim, Kim Young-Kee
exaly +2 more sources
Enzyme and Microbial Technology, 2009
Effects of initial medium pH and gas flow rate on Clostridium ljungdahlii and Clostridium autoethanogenum in liquid batch, continuous gas fermentations were investigated. Synthesis gas components were supplied at varying flow rates (5, 7.5 and 10 mL/min) for C. ljungdahlii (pH 6.8 and 5.5) and C. autoethanogenum (pH 6.0).
Jacqueline L. Cotter +2 more
openaire +1 more source
Effects of initial medium pH and gas flow rate on Clostridium ljungdahlii and Clostridium autoethanogenum in liquid batch, continuous gas fermentations were investigated. Synthesis gas components were supplied at varying flow rates (5, 7.5 and 10 mL/min) for C. ljungdahlii (pH 6.8 and 5.5) and C. autoethanogenum (pH 6.0).
Jacqueline L. Cotter +2 more
openaire +1 more source
Biotechnology and Bioprocess Engineering, 2020
The cost of culture media represents a considerable portion of the overall cost in syngas fermentation. Yeast extract is an expensive ingredient among the medium components required to prepare the culture medium. In this study, we evaluated the effectiveness of replacing yeast extract with relatively low-cost nutrient supplements such as corn steep ...
Hien Nguyen Thi +3 more
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The cost of culture media represents a considerable portion of the overall cost in syngas fermentation. Yeast extract is an expensive ingredient among the medium components required to prepare the culture medium. In this study, we evaluated the effectiveness of replacing yeast extract with relatively low-cost nutrient supplements such as corn steep ...
Hien Nguyen Thi +3 more
openaire +1 more source
FermentationProviding simultaneously autotrophic and heterotrophic carbon sources is a promising strategy to overcome the limits of autotrophic syngas fermentations. D-xylose and L-arabinose are particularly interesting as they can be obtained by the hydrolysis of lignocellulosic biomass.
Dirk Weuster-Botz +2 more
exaly +4 more sources
Progress towards platform chemical production using Clostridium autoethanogenum
Biochemical Society Transactions, 2018Since 2013, there has been an explosion in the number of research articles published on Clostridium autoethanogenum, an acetogen capable of producing platform chemicals such as ethanol and 2,3-butanediol from greenhouse gases. However, no review focusing solely on C. autoethanogenum has appeared in the literature.
Rupert O J, Norman +4 more
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