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Expansion of the genome editing toolbox of Clostridium autoethanogenum
2022Current environmental crises – from rising global temperatures to biodiversity collapse – have created an urgent need for new, circular and sustainable ways of producing and recycling materials and energy independently of fossil resources. Gas fermentation, by consuming greenhouse gases to produce fuels and chemicals, offers human society an attractive
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Enzyme and Microbial Technology, 2017
Bioconversion of CO/syngas to produce ethanol is a novel route in bioethanol production, which can be accomplished by some acetogens. Specific culture vessels and techniques are needed to cultivate these microorganisms since they are anaerobic and substrates are gaseous. In this work, gas-sampling bag was applied as a gas-cultivation system to study CO/
Xu, Huijuan +5 more
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Bioconversion of CO/syngas to produce ethanol is a novel route in bioethanol production, which can be accomplished by some acetogens. Specific culture vessels and techniques are needed to cultivate these microorganisms since they are anaerobic and substrates are gaseous. In this work, gas-sampling bag was applied as a gas-cultivation system to study CO/
Xu, Huijuan +5 more
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Metabolic engineering of Clostridium autoethanogenum
2016Gas fermentation has emerged as a promising technology that converts waste gases containing CO, CO2 and H2 (also known as syngas) into fuels and chemical commodities. Employed by LanzaTech Inc., Clostridium autoethanogenum is an industrial acetogen that converts gases into ethanol, 2,3-butanediol, acetate, and lactate.
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B-vitamin requirements of Clostridium autoethanogenum
2018Vitamins are micronutrients essential for life in an organism which must be obtained from diet or environment. Standard media often contains ten B-vitamins, which act as cofactors or precursors for co-enzymes. Clostridium autoethanogenum and Clostridium ljungdahlii are two obligately anaerobic acetogens which can utilize syngas (carbon dioxide ...
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International Journal of Biological Macromolecules
Clostridium autoethanogenum, a key organism for syngas fermentation, has great industrial potential as an anaerobic microbe. However, tools for monitoring and characterizing gene expression, such as fluorescent protein-based anaerobic reporters (FPARs), and promoter libraries for regulating expression intensity, are lacking. In this study, we developed
Yida, Zhai +9 more
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Clostridium autoethanogenum, a key organism for syngas fermentation, has great industrial potential as an anaerobic microbe. However, tools for monitoring and characterizing gene expression, such as fluorescent protein-based anaerobic reporters (FPARs), and promoter libraries for regulating expression intensity, are lacking. In this study, we developed
Yida, Zhai +9 more
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Biotechnology Journal
ABSTRACT Methylene‐tetrahydrofolate reductase (MTHFR) is an important enzyme for acetogenic carbon fixation, but the redox mechanism driving this reaction is not clearly understood. Previous enzymology work and energetic accounting on species such as Clostridium autoethanogenum
Lucas W. Mendelson +4 more
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ABSTRACT Methylene‐tetrahydrofolate reductase (MTHFR) is an important enzyme for acetogenic carbon fixation, but the redox mechanism driving this reaction is not clearly understood. Previous enzymology work and energetic accounting on species such as Clostridium autoethanogenum
Lucas W. Mendelson +4 more
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Characterisation of Agr quorum sensing in Clostridium autoethanogenum
2018The Gram-positive, anaerobic, acetogenic bacterium Clostridium autoethanogenum is regarded as an important biocatalyst in the current advancement of industrial gas fermentation. The ever-widening diversity of industrially-relevant acetogenic bacteria has inspired a rational approach into utilising industrial waste gases as a viable feedstock, with ...
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