Results 31 to 40 of about 1,327 (168)

Exploring the Mechanism of Clostridium autoethanogenum Protein for Broiler Growth Based on Gut Microbiota and Serum Metabolomics [PDF]

Biology
Intestinal health is vital for poultry production, and protein plays a key role in intestinal nutrition. The present study used 16S rRNA gene sequencing and serum metabolomics to investigate the effect of CAP on the cecal microflora structure and serum ...
Chunqiao Shan, Yan Liu, Sisi Liu, Chuang Li, Chaoxin Ma, Hongmin Yu, Juan Li, Guotuo Jiang, Jing Tian   +8 more
doaj   +2 more sources

Transcriptional control of Clostridium autoethanogenum using CRISPRi. [PDF]

Synth Biol (Oxf), 2021
Abstract Gas fermentation by Clostridium autoethanogenum is a commercial process for the sustainable biomanufacturing of fuels and valuable chemicals using abundant, low-cost C1 feedstocks (CO and CO2) from sources such as inedible biomass, unsorted and nonrecyclable municipal solid waste, and industrial emissions. Efforts toward pathway
Fackler N, Heffernan J, Juminaga A, Doser D, Nagaraju S, Gonzalez-Garcia RA, Simpson SD, Marcellin E, Köpke M.   +8 more
europepmc   +4 more sources

Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum. [PDF]

Appl Environ Microbiol, 2022
Although microbial genome sequences are relatively easily determined, assigning gene function remains a bottleneck. Consequently, relatively few genes are well characterized, leaving the function of many as either hypothetical or entirely unknown.
Woods C, Humphreys CM, Tomi-Andrino C, Henstra AM, Köpke M, Simpson SD, Winzer K, Minton NP.   +7 more
europepmc   +3 more sources

Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum. [PDF]

Appl Microbiol Biotechnol, 2019
Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reported, we observed that for growth, the addition of vitamins to media already containing yeast extract was ...
Annan FJ, Al-Sinawi B, Humphreys CM, Norman R, Winzer K, Köpke M, Simpson SD, Minton NP, Henstra AM.   +8 more
europepmc   +4 more sources

Genome editing of Clostridium autoethanogenum using CRISPR/Cas9. [PDF]

Biotechnol Biofuels, 2016
Impactful greenhouse gas emissions abatement can now be achieved through gas fermentation using acetogenic microbes for the production of low-carbon fuels and chemicals. However, compared to traditional hosts like Escherichia coli or yeast, only basic genetic tools exist for gas-fermenting acetogens. To advance the process, a robust genetic engineering
Nagaraju S, Davies NK, Walker DJ, Köpke M, Simpson SD.   +4 more
europepmc   +4 more sources

Redox controls metabolic robustness in the gas-fermenting acetogen Clostridium autoethanogenum. [PDF]

Proc Natl Acad Sci U S A, 2020
Living biological systems display a fascinating ability to self-organize their metabolism. This ability ultimately determines the metabolic robustness that is fundamental to controlling cellular behavior. However, fluctuations in metabolism can affect cellular homeostasis through transient oscillations.
Mahamkali V, Valgepea K, de Souza Pinto Lemgruber R, Plan M, Tappel R, Köpke M, Simpson SD, Nielsen LK, Marcellin E.   +8 more
europepmc   +6 more sources

Overflow metabolism at the thermodynamic limit of life: How carboxydotrophic acetogens mitigate carbon monoxide toxicity

Microbial Biotechnology, 2023
Carboxydotrophic metabolism is gaining interest due to its applications in gas fermentation technology, enabling the conversion of carbon monoxide to fuels and commodities.
Maximilienne T. Allaart, Martijn Diender, Diana Z. Sousa, Robbert Kleerebezem   +3 more
doaj   +1 more source

Specific detection of “Clostridium autoethanogenum”, Clostridium ljungdahlii and Clostridium carboxidivorans in complex bioreactor samples [PDF]

FEMS Microbiology Letters, 2018
The high genetic similarity between some carboxydotrophic bacteria does not allow for the use of common sequencing techniques targeting the 16S rRNA gene for species identification. 16S rRNA sequencing fails to discriminate among Clostridium ljungdahlii and 'Clostridium autoethanogenum', despite this two species exhibit significant differences in CO2 ...
Ester Cantos-Parra, Sara Ramió-Pujol, Jesús Colprim, Sebastià Puig, Lluís Bañeras   +4 more
openaire   +2 more sources

Screening of Gas Substrate and Medium Effects on 2,3-Butanediol Production with C. ljungdahlii and C. autoethanogenum Aided by Improved Autotrophic Cultivation Technique

Fermentation, 2021
Gas fermentation by acetogens of the genus Clostridium is an attractive technology since it affords the production of biochemicals and biofuels from industrial waste gases while contributing to mitigate the carbon cycle alterations.
Luca Ricci, Valeria Agostino, Debora Fino, Angela Re   +3 more
doaj   +1 more source

Multi-omics approach to study the dual effects of novel proteins on the intestinal health of juvenile largemouth bass (Micropterus salmoides) under an alternate feeding strategy

Frontiers in Immunology, 2023
IntroductionIn an effort to minimize the usage of fishmeal in aquaculture, novel protein diets, including Tenebrio molitor, cottonseed protein concentrate, Clostridium autoethanogenum, and Chlorella vulgaris were evaluated for their potential to replace ...
Lukuan Li, Yu Wang, Yanqing Huang, Chunfang Wang   +3 more
doaj   +1 more source