Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
An Explanatory Model of Red Lentil Seed Coat Colour to Manage Degradation in Quality during Storage
AgronomyThis study presents an explanatory biophysical model developed and validated to simulate seed coat colour traits including CIE L*, a*, and b* changes over time for stored lentil cultivars PBA Hallmark, PBA Hurricane, PBA Bolt, and PBA Jumbo2 under ...
Bhawana Bhattarai +5 more
doaj +1 more source
Genetic polymorphism of adult reindeer coat colour in a herding cooperative in Finnish Lapland
Rangifer, 2011 In a random sample of 188 adult reindeer belonging to a reindeer herding cooperative in Finnish Lapland, the following coat colour mutants were identified: Abf at the locus Agouti (A), kalppinokka (WNk) at the locus White Nose (WN) and white at the locus
Jean J. Lauvergne, Mauri Nieminen
doaj +1 more source
Coat colour inheritance in horses
, 2012 Gerçekleştirilen bu derleme çalışmasında atların don kalıtımı özetlenmeye çalışılmıştır. Bir atın don ve nişanelerinin tanımlanması, o atın bir birey olarak tanımlanması bakımından önemlidir.
Yılmaz, Orhan +2 more
core
Green seed coat colour retention in lentil (lens culinaris) [PDF]
, 2007 Poor seed coat colour desirability in green lentil (Lens culinaris) costs lentil producers millions of dollars each year. The monetary value that Canadian lentil producers receive for their crop is based on the visual characteristics of the seed coat ...
Davey, Blaine Frederic
core
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Altitude, latitude and climate zone as determinants of mountain hare (Lepus timidus) coat colour change. [PDF]
Ecol Evol, 2023 Stokes AW +5 more
europepmc +1 more source
MiR‐513a promotes human erythroid differentiation by modulating c‐Jun
FEBS Open Bio, EarlyView.During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim +11 more
wiley +1 more source
Seed coat colour and structure are related to the seed dormancy and overwintering ability of crop-to-wild hybrid soybean. [PDF]
AoB Plants, 2023 Zhang L +6 more
europepmc +1 more source