Results 91 to 100 of about 15,890 (276)

Dual pKa Lipid Nanoparticles for Lung‐tropic mRNA Delivery and pH‐Programmed Endosomal Escape

Advanced Science, EarlyView.
Lipid nanoparticles’ bottleneck lies in low endosomal escape efficiency and liver‐dominant delivery after intravenous administration. To overcome these limitations, we present a stepwise pH‐programmed lipid nanoparticle system enabled by our newly synthesized ionizable lipid.
Seong Gi Lim, Minju Lee, Yiming Wang, Eunjin Choi, Ji Sun Park, Kyungmin Park, Hayoung Jeon, Donghyun Kim, Donghyun Lee, Yeeun Lee, Kee‐Pyo Kim, Eunha Kim, Heebeom Koo   +12 more
wiley   +1 more source

Coenzyme Acceptance in Ene‐Reductases From Herbaspirillum seropedicae

ChemistryEurope
Ene‐reductases (EREDs) are commonly employed for the asymmetric synthesis of important industrial chemicals. In this research, we characterise two EREDs (HSI and HSII) belonging to the ‘thermophilic‐like’ Old Yellow Enzyme (OYE) family from the beta ...
Karishma Shah, Silvia Wallner, Aleksandar Bijelic, André Pick, Peter Macheroux, Robert Kourist, Daniel Kracher   +6 more
doaj   +1 more source

OAF Blocks SIAH1‐Mediated Degradation of SCPX, a Therapeutic Strategy for MASLD

Advanced Science, EarlyView.
This study shows OAF directly binds to SCPX and inhibits its interaction with the E3 ligase SIAH1, thereby protecting SCPX from ubiquitin‐dependent degradation and stabilizing its levels. This OAF‐SCPX axis represents a novel pathway in lipid homeostasis, highlighting OAF as a promising therapeutic candidate for MASLD.
Zongxi Li, Jingyue Song, Kaili Ma, Shunan Tang, Shuqian Wang, Wenya Ji, Chen Lu, Ting Zhang, Huaping Zheng, Xiaowei Zheng, Shanshan Lai, Cheng Deng   +11 more
wiley   +1 more source

Cysteine Thioaldehydes: Photolytic Generation, Reactivity, and Biological Implications

Angewandte Chemie, EarlyView.
Photolysis of cysteine phenacylsulfides bearing non‐conjugating electron‐withdrawing substituents leads to high conversions into cysteine thioaldehydes through a Norrish type‐II pathway. This methodology enabled the study of the aqueous reactivity of these important biosynthetic intermediates, which, depending on peptide sequence, pH and buffer ...
Ardra Karthika, Matthew Murray, Guillermo Palop, Lee‐Ann J. Keane, Patrick McArdle, Nicholas D. J. Yates, Christopher D. Spicer, Alison Parkin, Martin A. Fascione, Eddie L. Myers   +9 more
wiley   +2 more sources

BOFdat Step 2: Identifying and calculating the stoichiometric coefficients of coenzymes and inorganic ions.

, 2019
(A) Pie graphs of the percent dry weight accounted for before and after BOFdat Step 2. (B) The coenzymes found by BOFdat Step 2 are metabolites with a higher degree than the established threshold (S1 Text).
Jean-Christophe Lachance (6619307), Colton J. Lloyd (5484473), Sébastien Rodrigue (139074), Bernhard O. Palsson (91399), Adam M. Feist (200740), Pierre-Étienne Jacques (145554), Jonathan M. Monk (2147158), Anand V. Sastry (6619310), Yara Seif (4908814), Laurence Yang (3440912), Zachary A. King (789115)   +10 more
core   +1 more source

A Specific Process to Purify 2-Methyl-D-Erythritol-4-Phosphate Enzymatically Converted from D-Glyceraldehyde-3-Phosphate and Pyruvate

Natural Product Communications, 2015
A one-pot enzymatic cascade was established to synthesize MEP, one of the key intermediates in the MEP terpenoid biosynthetic pathway. D-GAP and sodium pyruvate were converted to MEP in a reaction catalyzed by DXP synthase and DXP reductoisomerase (DXR ...
Shao-Qing Yang, Jian Deng, Qian-Qian Wu, Heng Li, Wen-Yun Gao   +4 more
doaj   +1 more source

DLST Succinylation‐Mediated Mitochondrial Metabolic Remodeling and Cuproptosis Resistance Promote Malignant Progression of Lung Adenocarcinoma

Advanced Science, EarlyView.
The DLST K409 succinylation mediated by CPT1A promotes OXPHOS and redox homeostasis through regulating enzyme activity, thereby promoting the malignant progression of LUAD. Notably, the succinylation of DLST can enhance the cuproptosis resistance by inhibiting its lipoylation.
Xuanxuan Li, Peijun Zhou, Xingzhi Peng, Qixin Liu, Wenhui Zeng, Rui Wang, Liangfang Shen, Jinwu Peng, Qin Zhou, Lifang Yang   +9 more
wiley   +1 more source

Isothiazologuanosine (tzG) as Environmentally Sensitive Fluorescent Reporter in RNA

Angewandte Chemie, EarlyView.
The emissive isothiazologuanosine analog tzG is synthesized for the first time as RNA phosphoramidite building block for solid‐phase synthesis. Due to its isomorphic and isofunctional characteristics, tzG forms Watson–Crick base pairs and can be used as fluorescent probe to monitor (deoxy)ribozyme activity.
Julia Dietzsch, Yitzhak Tor, Claudia Höbartner   +2 more
wiley   +2 more sources

Integration of the metabolic/redox state, histone gene switching, DNA replication and S-phase progression by moonlighting metabolic enzymes

Bioscience Reports, 2013
The concept of one-protein–multiple-function, i.e. moonlighting proteins, is an ever-expanding paradigm. We obtained compelling evidence that an array of ‘cytoplasmic’ metabolic enzymes can enter the nuclei to carry out moonlighting ...
Hongpeng He, Mei‑Chin Lee, Li‑Ling Zheng, Lei Zheng, Yan Luo   +4 more
doaj   +1 more source

Multifunctional Nanovaccine‐Radiotherapy Synergy Drives Anti‐Tumor Immunity via Multi‐Cascade Cell Death for Pancreatic Cancer Therapy

Advanced Science, EarlyView.
Machine‐learning‐driven transcriptomic analysis identifies FSTL3 as a key tumor‐microenvironment regulator in desmoplastic pancreatic adenocarcinoma. A cRGD‐modified liposome co‐loading gemcitabine and siFSTL3 serves as an irradiation‐triggered in situ nanovaccine. It synergizes with radiotherapy to induce ferroptosis and immunogenic cell death through
Chengyi Huang, Yushu Han, Xiang Liu, Chuanhao Wang, Xiaofei Zhu, Di Chen, Yangyang Gong, Liang Chen, Wenjuan Chen, Zhenyuan Miao, Bufu Tang, Huojun Zhang   +11 more
wiley   +1 more source