Results 61 to 70 of about 12,584,658 (247)
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
MATEC Web of Conferences, 2016 A new cold rotary forging technology of the internal helical involute spline was presented based on an analysis of the structure of automotive starter guide cylinder. 3D rigid-plastic finite element model was employed.
Wang Ping, Dong Xiao Fei
doaj +1 more source
Modelling hot (metal) sheet forming with cold tooling
, 2012 Use of cold tooling is beneficial in the sheet metal forming. This paper presents simulations of the process with the objective of investigating issues associated with thermal management, and of providing thermo-mechanical data for future ...
Spence, Julian +4 more
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FEBS Open Bio, EarlyView.KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph +7 more
wiley +1 more source
MetalsIn order to reduce the edge waves and defects of the strip in the forming process and obtain better properties of the strip, it is urgent to establish a better flexible cold forming process.
Shengde Hu, Junhao Zhao, Yu Liu
doaj +1 more source
Validation of the New Mix Design Process for Cold In-Place Rehabilitation Using Foamed Asphalt, 2007 [PDF]
, 2007 Asphalt pavement recycling has grown dramatically over the last few years as a viable technology to rehabilitate existing asphalt pavements. Iowa's current Cold In-place Recycling (CIR) practice utilizes a generic recipe specification to define the ...
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Numerical simulation on chain-die forming of an AHSS top-hat section [PDF]
, 2018 The applications of Advanced High-Strength Steels (AHSS) in the automotive industry are rapidly increasing due to a demand for a lightweight material that significantly reduces fuel consumption without compromising passenger safety. Automotive industries
Ding, Scott +10 more
core +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Manufacturing Review, 2019 Cold metal forming is a fast and economical way of producing a wide range of precise components. Its profitability mainly depends on part quality, process stability, and service intervals of tools.
Tröber Philipp +5 more
doaj +1 more source