Results 121 to 130 of about 9,867,423 (273)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

The protective role of PYY in intestinal mucosal defects induced by SATB2 deficiency in inflammatory bowel disease

Cell Death Discovery
Impaired colonic mucosal repair is a critical issue in inflammatory bowel diseases (IBD). SATB2 is essential for maintaining colonic epithelial homeostasis, but its role in mucosal repair is unclear. In this study, flow cytometry was used to assess SATB2’
Yao Liu, Lanqing Wu, Xiaoli Li, Yongyu Chen, Ruidong Chen, Caiyun Lv, Juan Chen, Xinjuan Fan, Guangxin Duan, Fan Zhong, Qi Sun, Qianyun Shi, Hengli Ni, Lina Sun, Jiaying Xu, Wen Tang, Jianming Li   +16 more
doaj   +1 more source

Replication of Colonic Crohn's Disease Mucosal Escherichia coli Isolates within Macrophages and Their Susceptibility to Antibiotics

, 2007
Sreedhar Subramanian, Carol L. Roberts, C. Anthony Hart, Helen Martin, Steve W. Edwards, Jonathan M. Rhodes, Barry J. Campbell   +6 more
openalex   +1 more source

Prophylaxis of uric acid stone in patients with inflammatory bowel disease following extensive colonic resection [PDF]

, 1991
Tsuneo Fukushima, Yasunobu Yamazaki, Akira Sugita, Shuji Tsuchiya   +3 more
openalex   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Oversecretion and Overexpression of Nicotinamide Phosphoribosyltransferase/Pre-B Colony-Enhancing Factor/Visfatin in Inflammatory Bowel Disease Reflects the Disease Activity, Severity of Inflammatory Response and Hypoxia [PDF]

, 2019
Katarzyna Neubauer, Iwona Bednarz‐Misa, Ewa Wałecka-Zacharska, Jarosław Wierzbicki, Anil Agrawal, Andrzej Gamian, Małgorzata Krzystek‐Korpacka   +6 more
openalex   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

Melatonin's role as a co‐adjuvant treatment in colonic diseases: A review

Life Science, 2017
E. Esteban-Zubero, L. López-Pingarrón, M. Alatorre-Jiménez, Purificación Ochoa‐Moneo, Celia Buisac‐Ramón, Miguel Rivas‐Jiménez, S. Castán-Ruiz, Á. Antoñanzas‐Lombarte, D. Tan, J. García, R. Reiter   +10 more
semanticscholar   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source