Results 1 to 10 of about 939 (180)

Using a human colonoid-derived monolayer to study bacteriophage translocation [PDF]

Gut Microbes
Bacteriophages (phages) are estimated to be the most abundant microorganisms on Earth. Their presence in human blood suggests that they can translocate from non-sterile sites such as the gastrointestinal tract where they are concentrated.
Huu Thanh Le, Alicia Fajardo Lubian, Bethany Bowring, David van der Poorten, Jonathan Iredell, Jacob George, Carola Venturini, Golo Ahlenstiel, Scott Read   +8 more
doaj   +5 more sources

Tofacitinib and budesonide treatment affect stemness and chemokine release in IBD patient-derived colonoids [PDF]

Scientific Reports
Restoration of the intestinal epithelial barrier is crucial for achieving mucosal healing, the therapeutic goal for inflammatory bowel disease (IBD). During homeostasis, epithelial renewal is maintained by crypt stem cells and progenitors that cease to ...
Arun Sridhar, Ingunn Bakke, Shreya Gopalakrishnan, Nimo Mukhtar Mohamud Osoble, Emilie Prytz Hammarqvist, Henrik P. Sahlin Pettersen, Arne Kristian Sandvik, Ann Elisabet Østvik, Marianne Doré Hansen, Torunn Bruland   +9 more
doaj   +4 more sources

Investigation of Colonic Regeneration via Precise Damage Application Using Femtosecond Laser-Based Nanosurgery [PDF]

Cells, 2022
Organoids represent the cellular composition of natural tissue. So called colonoids, organoids derived from colon tissue, are a good model for understanding regeneration.
Sören Donath, Leon Angerstein, Lara Gentemann, Dominik Müller, Anna E. Seidler, Christian Jesinghaus, André Bleich, Alexander Heisterkamp, Manuela Buettner, Stefan Kalies   +9 more
doaj   +4 more sources

Comprehensive protocols for culturing and molecular biological analysis of IBD patient-derived colon epithelial organoids [PDF]

Frontiers in Immunology, 2023
There are many unanswered questions regarding responses to proinflammatory signals in intestinal epithelial cells (IECs). For example, chemokines secreted by IECs upon external stimuli play multifunctional roles in both homeostasis and during ...
Shreya Gopalakrishnan, Ingunn Bakke, Ingunn Bakke, Marianne Doré Hansen, Marianne Doré Hansen, Helene Kolstad Skovdahl, Helene Kolstad Skovdahl, Atle van Beelen Granlund, Atle van Beelen Granlund, Atle van Beelen Granlund, Atle van Beelen Granlund, Arne K. Sandvik, Arne K. Sandvik, Arne K. Sandvik, Torunn Bruland, Torunn Bruland, Torunn Bruland   +16 more
doaj   +3 more sources

Identification and pharmacological characterization of a natural folipastatin as a CFTR inhibitor and potential anti-secretory agent in a human colonoid model [PDF]

BMC Complementary Medicine and Therapies
Background Overstimulation of cAMP-induced intestinal chloride secretion is a major cause of secretory diarrheas affecting both children and adults. This study aimed to investigate an anti-secretory effect of folipastatin, a natural depsidone derived ...
Saravut Satitsri, Rungtiwa Khumjiang, Nichakorn Worakajit, Bodee Nutho, Thanyada Rungrotmongkol, Patima Phainuphong, Taya Kitiyakara, Vatcharin Rukachaisirikul, Chatchai Muanprasat   +8 more
doaj   +2 more sources

Patient-derived colon epithelial organoids reveal lipid-related metabolic dysfunction in pediatric ulcerative colitis [PDF]

Nature Communications
Ulcerative colitis (UC) is associated with epithelial metabolic derangements which exacerbate gut inflammation. Here, we develop colon organoid (colonoid) lines from pediatric patients with endoscopically active UC, inactive UC, and those without ...
Babajide A. Ojo, Ying Zhu, Lyong Heo, Sejal R. Fox, Yanli Qiao, Amanda Waddell, Maria E. Moreno-Fernandez, Marielle Gibson, Tracy Tran, Ashley L. Dunn, Essam I. A. Elknawy, Neetu Saini, Javier A. López-Rivera, Senad Divanovic, Yuqin Dai, Vinicio A. de Jesus Perez, Michael J. Rosen   +16 more
doaj   +2 more sources

The natural statin α,β-dehydromonacolin K exerts anti-secretory effect in human intestinal epithelial cells via a nonsense-mediated mRNA decay-dependent mechanism [PDF]

Pharmaceutical Biology
Context cAMP-induced intestinal chloride secretion plays a pivotal role in the pathogenesis of secretory diarrheas.Objective In this study, we investigated the antisecretory effects of α,β-dehydromonacolin K, a derivative of lovastatin from Aspergillus ...
Saravut Satitsri, Rungtiwa Khumjiang, Chittreeya Tansakul, Wararat Chiangjong, Nuttapon Apichaiyarat, Taya Kitiyakara, Yanisa Purintrapibal, Vatcharin Rukachaisirikul, Chatchai Muanprasat   +8 more
doaj   +2 more sources

Enterohemorrhagic Escherichia coli (EHEC) disrupts intestinal barrier integrity in translational canine stem cell-derived monolayers [PDF]

Microbiology Spectrum
This study addresses the gap in translatable in vitro models for investigating Enterohemorrhagic E. coli (EHEC) infections, particularly relevant to both canine and human health.
Itsuma Nagao, Minae Kawasaki, Takashi Goyama, Hyun Jung Kim, Douglas R. Call, Yoko M. Ambrosini   +5 more
doaj   +2 more sources

Inhibition of CFTR-mediated intestinal chloride secretion by nornidulin: Cellular mechanisms and anti-secretory efficacy in human intestinal epithelial cells and human colonoids. [PDF]

PLoS ONE
Secretory diarrhea, a major global health concern, particularly among young children, is often characterized by excessive chloride secretion through the cystic fibrosis transmembrane conductance regulator (CFTR) channel.
Chamnan Yibcharoenporn, Thidarat Kongkaew, Nichakorn Worakajit, Rungtiwa Khumjiang, Praphatsorn Saetang, Saravut Satitsri, Vatcharin Rukachaisirikul, Chatchai Muanprasat   +7 more
doaj   +2 more sources

Murine colon organoids as a novel model to study Trypanosoma cruzi infection and interactions with the intestinal epithelium [PDF]

Frontiers in Cellular and Infection Microbiology, 2023
Chagas disease (CD) is a life-threatening illness caused by the parasite Trypanosoma cruzi (T. cruzi). With around seven million people infected worldwide and over 50,000 deaths per year, CD is a major public health issue in Latin America. The main route
Hellen Daghero, Romina Pagotto, Cristina Quiroga, Andrea Medeiros, Andrea Medeiros, Marcelo A. Comini, Mariela Bollati-Fogolín   +6 more
doaj   +4 more sources