Immunofluorescent Staining Assay of 3D Cell Culture of Colonoids Isolated from Mice Colon [PDF]
Bio-ProtocolHere, we describe immunofluorescent (IF) staining assay of 3D cell culture colonoids isolated from mice colon as described previously. Primary cultures developed from isolated colonic stem cells are called colonoids.
Trisha Mehrotra +2 more
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Transcriptional responses of mouse proximal colon and colonoids during early whipworm infection [PDF]
mBioTrichuriasis, caused by the parasitic nematode Trichuris trichiura, affects 429–508 million people worldwide. Although the early phase of whipworm infection is crucial for establishing infection, its underlying molecular mechanisms remain unclear.
Hyeim Jung +3 more
doaj +4 more sources
Porcine colonoids and enteroids keep the memory of their origin during regeneration [PDF]
American Journal of Physiology-Cell Physiology, 2021 The development of alternative in vitro culture methods has increased in the last decade as three-dimensional organoids of various tissues, including those of the small and large intestines.
Barnett AM +5 more
core +5 more sources
Patient derived colonoids as drug testing platforms - Critical importance of oxygen concentration [PDF]
Frontiers in Pharmacology, 2021 Treatment of inflammatory bowel disease (IBD) is challenging, with a series of available drugs each helping only a fraction of patients. Patients may face time-consuming drug trials while the disease is active, thus there is an unmet need for biomarkers ...
Bakke, Ingunn +12 more
core +9 more sources
Ulcerative Colitis-Derived Colonoid Culture: A Multi-Mineral-Approach to Improve Barrier Protein Expression [PDF]
Frontiers in Cell and Developmental Biology, 2020 BackgroundRecent studies demonstrated that Aquamin®, a calcium-, magnesium-rich, multi-mineral natural product, improves barrier structure and function in colonoids obtained from the tissue of healthy subjects.
Muhammad N. Aslam +11 more
doaj +4 more sources
Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids [PDF]
Neurogastroenterology & Motility, 2022 Background: Alteration of the host-microbiota cross talk at the intestinal barrier may participate in the pathophysiology of irritable bowel syndrome (IBS).
6hman, Lena +8 more
core +4 more sources
Efficient RNA and RNA-protein co-detection in 3D colonoids by whole-mount staining
STAR Protocols, 2022 Summary: Here, we describe a protocol to visualize RNA oligos and proteins independently or together using a combination of fluorescence in situ hybridization (FISH) and immunofluorescence in human colonoids, expanding on previously published research ...
Roger Atanga +2 more
doaj +3 more sources
Initial pig developmental stage influences intestinal organoid growth but not cellular composition
Animal Models and Experimental MedicineBackground Intestinal organoids are promising tools in the context of animal experiment reduction but a thorough characterization of the impact of the origin of intestinal stem cells (ISC) on organoid phenotype is needed to routinely use this cellular ...
Camille Duchesne +6 more
doaj +2 more sources
Near-Infrared In Vivo Imaging of Claudin-1 Expression by Orthotopically Implanted Patient-Derived Colonic Adenoma Organoids [PDF]
DiagnosticsBackground: Claudin-1 becomes overexpressed during the transformation of normal colonic mucosa to colorectal cancer (CRC). Methods: Patient-derived organoids expressed clinically relevant target levels and genetic heterogeneity, and were established from
Sangeeta Jaiswal +8 more
doaj +2 more sources
In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment. [PDF]
PLoS ONE, 2016 The polarity of proliferative and differentiated cellular compartments of colonic crypts is believed to be specified by gradients of key mitogens and morphogens.
Peter J Attayek +6 more
doaj +4 more sources