Physiological hypoxia improves growth and functional differentiation of human intestinal epithelial organoids [PDF]
, 2023 Background: The epithelium in the colonic mucosa is implicated in the pathophysiology of various diseases, including inflammatory bowel diseases and colorectal cancer.
Bakke, Ingunn +7 more
core +1 more source
IL-27 induces an IFN-like signature in murine macrophages which in turn modulate colonic epithelium
Frontiers in Immunology, 2023 Mucosal delivery of IL-27 has been shown to have a therapeutic benefit in murine models of inflammatory bowel disease (IBD). The IL-27 effect was associated with phosphorylated STAT1 (pSTAT1), a product of IL27 receptor signaling, in bowel tissue.
Caroline Andrews +14 more
doaj +1 more source
Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli [PDF]
Scientific Reports, 2020 AbstractEAEC is a common cause of diarrheal illness worldwide. Pathogenesis is believed to occur in the ileum and colon, where the bacteria adhere and form a robust aggregating biofilm. Among the multiple virulence factors produced by EAEC, the Pic serine protease has been implicated in bacterial colonization by virtue of its mucinolytic activity ...
Liu, Lixia +9 more
openaire +2 more sources
Cell-Matrix Interactions Contribute to Barrier Function in Human Colon Organoids
Frontiers in Medicine, 2022 The importance of cell-matrix adhesion to barrier control in the colon is unclear. The goals of the present study were to: (i) determine if disruption of colon epithelial cell interactions with the extracellular matrix alters permeability control ...
James Varani +2 more
doaj +1 more source
Modeling inflammation and oxidative stress in gastrointestinal disease development using novel organotypic culture systems. [PDF]
, 2013 Gastroesophageal reflux disease (GERD), Barrett's esophagus (BE), graft-versus-host disease (GVHD), and inflammatory bowel diseases such as ulcerative colitis and Crohn's disease are common human gastrointestinal diseases that share inflammation as a key
Bortner, James D +6 more
core +4 more sources
Cells, 2023 A key component of efforts to identify the biological and drug-specific aspects contributing to therapeutic failure or unexpected exposure-associated toxicity is the study of drug–intestinal barrier interactions. While methods supporting such assessments
Dipak Kumar Sahoo +12 more
doaj +1 more source
Epithelial restitution in 3D - Revealing biomechanical and physiochemical dynamics in intestinal organoids via fs laser nanosurgery [PDF]
, 2023 Intestinal organoids represent a three-dimensional cell culture system mimicking the mammalian intestine. The application of single-cell ablation for defined wounding via a femtosecond laser system within the crypt base allowed us to study cell dynamics ...
Bleich, André +12 more
core +2 more sources
Metabolites, 2021 Colonic epithelium–commensal interactions play a very important role in human health and disease development. Colonic mucus serves as an ecologic niche for a myriad of commensals and provides a physical barrier between the epithelium and luminal content,
Karol Dokladny +4 more
doaj +1 more source
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy [PDF]
Journal of Visualized Experiments, 2015 The epithelium of the gastrointestinal tract is constantly renewed as it turns over. This process is triggered by the proliferation of intestinal stem cells (ISCs) and progeny that progressively migrate and differentiate toward the tip of the villi.
Mahe, Maxime M. +4 more
openaire +3 more sources
Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters [PDF]
, 2022 Hypoxia exerts profound effects on cell physiology, but its effect on colonic uptake of the microbiota-generated forms of vitamin B1 (i.e., thiamin pyrophosphate [TPP] and free thiamine) has not been described.
Fleckenstein, James M +5 more
core +3 more sources